FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-209-6-5-7-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-209-6-5-7-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172794
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC4147424.001990809866083No Hit
CTGGATGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACCTAGCTG2697415.610495734805607No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAAC83064.8068798685139535No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT72934.22063266085628No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT50542.924870076507286No Hit
CTGGATGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACCTAGCT40142.322997326296052No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA36812.130282301468801No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT33821.957243885783071No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT28721.6620947486602544No Hit
CTGGATGACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTACCTAGC26041.5069967707212055No Hit
CTGGATGACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTACCTAGC22801.319490260078475No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC19311.1175156544787435No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT19031.1013113881269025No Hit
CTGGATGACTGGGATTAGATACCCCGAGAGAGGAGCCTGTTCTACCTAGC17901.035915598921259No Hit
CTGGATGACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTACC15870.9184346678704122No Hit
CTGGATGACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAC15360.8889197541581305No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGAT14960.8657708022269293No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC14260.8252601363473269No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAC12870.7448175283864023No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCGTAAACTCAGAC11370.6580089586443973No Hit
CTGGATGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT11180.6470132064770767No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGAT10670.6174982927647951No Hit
CTGGATGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC10290.5955067884301538No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT9850.5700429413058323No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAGT8540.4942301237311481No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA8360.4838130953621075No Hit
CTGGATGACTGGGATTAGATACCCCCTATGTATAGAGGAGCCTGTTCTAC8250.4774471335810271No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCACGAGACCTAGAGGAGCCTG7970.46124286722918617No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT7620.44098753428938503No Hit
CTGGATGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC7570.43809391529798486No Hit
CTGGATGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT7490.43346412491174463No Hit
CTGGATGACTGGGATTAGATACCCCGTCTCGTGTCTAGAGGAGCCTGTTC7430.4299917821220644No Hit
CTGGATGACTGGGATTAGATACCCCCCAATTTTCCTCTAGAGGAGCCTGT7420.42941305832378435No Hit
CTGGATGACTGGGATTAGATACCCCGATGTATAGAGGAGCCTGTTCTACC7290.421889648946144No Hit
CTGGATGACTGGGATTAGATACCCCGAGACCACGAGACTAGAGGAGCCTG7230.4184173061564637No Hit
CTGGATGACTGGGATTAGATACCCCCGTCGGCACTAGAGGAGCCTGTTCT6940.4016343160063428No Hit
CTGGATGACTGGGATTAGATACCCCCGGATATAGAGGAGCCTGTTCTACC6870.39758324941838263No Hit
CTGGATGACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTACCT6680.38658749725106195No Hit
CTGGATGACTGGGATTAGATACCCCGAGACAGAGGAGCCTGTTCTACCTA6570.3802215354699816No Hit
CTGGATGACTGGGATTAGATACCCCTATAAAGGGCTAGAGGAGCCTGTTC6330.3663321643112608No Hit
CTGGATGACTGGGATTAGATACCCCTTAATACGACTCACTAGAGGAGCCT6310.36517471671470075No Hit
CTGGATGACTGGGATTAGATACCCCGTATAGAGGAGCCTGTTCTACCTAG6300.3645959929164207No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACGAGGAGCCTGTTCTACCT6130.3547576883456601No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGAT5860.33913214579209927No Hit
CTGGATGACTGGGATTAGATACCCCCCCTAGAGGAGCCTGTTCTACCTAG5530.3200342604488582No Hit
CTGGATGACTGGGATTAGATACCCCTTCGTCGGCTAGAGGAGCCTGTTCT5250.3038299940970172No Hit
CTGGATGACTGGGATTAGATACCCCAAGACCTAGAGGAGCCTGTTCTACC5200.3009363751056171No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAAC4860.28125976596409596No Hit
CTGGATGACTGGGATTAGATACCCCGAGACCACGAGACGAGGAGCCTGTT4850.28068104216581596No Hit
CTGGATGACTGGGATTAGATACCCCTCTCGCCGTCAGATGTGTATAAGAG4660.26968528999849534No Hit
CTGGATGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACCTAG4560.263898052015695No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGAT4440.2569533664363346No Hit
CTGGATGACTGGGATTAGATACCCTCGTGGCAGCGTCAGATGTGTATAGA4420.25579591883977454No Hit
CTGGATGACTGGGATTAGATACCCCCTCAGAGGAGCCTGTTCTACCTAGC4220.24422144287417386No Hit
CTGGATGACTGGGATTAGATACCCCTAAGGAGCCTGTTCTACCTAGCTGT3830.2216512147412526No Hit
CTGGATGACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTACCT3740.21644270055673231No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGTCGTAAACCCTAAT3640.21065546257393197No Hit
CTGGATGACTGGGATTAGATACCCCCTATGCTTAGCCCTAAACCTCAACA3580.20718311978425177No Hit
CTGGATGACTGGGATTAGATACCCCTGTGTATAGAGGAGCCTGTTCTACC3510.20313205319629152No Hit
CTGGATGACTGGGATTAGATACCCCCGTGCTAGAGGAGCCTGTTCTACCT3480.20139588180145143No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT3340.19329374862553098No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAACTCTAAACTCGAAT3300.19097885343241086No Hit
CTGGATGACTGGGATTAGATACCCCGTCTGTGGGCTAGAGGAGCCTGTTC3300.19097885343241086No Hit
CTGGATGACTGGGATTAGATACCCGTCTCGTGTCTCTAGAGGAGCCTGTT3250.1880852344410107No Hit
CTGGATGACTGGGATTAGATACCCTAGAGGAGCCTGTTCTACCTAGCTGT3240.18750651064273063No Hit
CTGGATGACTGGGATTAGATACCCCTCTCGTGGGCTAGAGGAGCCTGTTC3120.18056182506337026No Hit
CTGGATGACTGGGATTAGATACCCCCGTAGAAGCTAGAGGAGCCTGTTCT2840.16435755871152935No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGACGTAAACCTTGAT2830.1637788349132493No Hit
CTGGATGACTGGGATTAGATACCCTCGTCTAGAGGAGCCTGTTCTACCTA2810.16262138731668924No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCCAC2780.16088521592184912No Hit
CTGGATGACTGGGATTAGATACCTAGAGGAGCCTGTTCTACCTAGCTGTC2720.15741287313216892No Hit
CTGGATGACTGGGATTAGATACCCTCGTCGTCGTCGTCTCGTCTAGAGGA2670.15451925414076878No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT2650.15336180654420872No Hit
CTGGATGACTGGGATTAGATACCCCGAGAGAAGCTAGAGGAGCCTGTTCT2580.1493107399562485No Hit
CTGGATGACTGGGATTAGATACCCCGAGGAGCCTGTTCTACCTAGCTGTC2560.1481532923596884No Hit
CTGGATGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA2550.14757456856140838No Hit
CTGGATGACTGGGATTAGATACCCCGACAGCTAGAGGAGCCTGTTCTACC2500.1446809495700082No Hit
CTGGATGACTGGGATTAGATACCCCCCCCTAGAGGAGCCTGTTCTACCTA2480.14352350197344815No Hit
CTGGATGACTGGGATTAGATACCCCGTCTCGAGGAGCCTGTTCTACCTAG2130.12326816903364701No Hit
CTGGATGACTGGGATTAGATACCCTCTAGAGGAGCCTGTTCTACCTAGCT1950.1128511406646064No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATAGAT1930.11169369306804634No Hit
CTGGATGACTGGGATTAGATACCCCGAGACAGGAGCCTGTTCTACCTAGC1800.10417028369040592No Hit
CTGGATGACTGGATTAGATACCCCTAGAGGAGCCTGTTCTACCTAGCTGT1760.10185538849728577No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGG108.4591605E-4294.995977
CTGGGAC108.4591605E-4294.995979
CTGGATG171550.0294.566041
TGACTGG170450.0294.476756
TGGATGA171500.0294.393922
GGATGAC171250.0294.306923
GACTGGG169500.0294.038737
GATGACT171350.0293.44654
ATGACTG171350.0293.188265
ACTGGGA169800.0292.91128
CTGGGAT169900.0292.738779
ACGGGAT301.5770638E-9245.829948
ACTGGGT600.0245.829948
CTGGATT1250.0224.196919
GACTGGA1550.0218.867977
TGGAGAC750.0216.330372
CGGGATT353.963578E-9210.711389
GACGGGA353.963578E-9210.711387
GGATACT850.0208.232443
AGACTGG800.0202.809725
Produced by FastQC (version 0.11.5)