FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-208-6-5-7-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-208-6-5-7-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171682
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC6902340.20398178026817No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT100175.834624480143521No Hit
CTGGATGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGATTGAGA96955.647068417189921No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT64593.762188231730758No Hit
CTGGATGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGATTGAG47332.756841136519845No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC33561.954776854882865No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA26081.5190876154751227No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT23601.3746344986661385No Hit
CTGGATGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT23561.3723046096853484No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT23111.3460933586514603No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC21611.258722521871833No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT21581.2569751051362403No Hit
CTGGATGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGATT21261.2383359932899196No Hit
CTGGATGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC19591.1410631283419344No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT17261.0053470952109131No Hit
CTGGATGACTGGGATTAGATACCCCAGCCTAGAGGAGCCTGTTCTAGATT16670.9709812327442597No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAG15530.9045793967917428No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT15020.8748733122866695No Hit
CTGGATGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGATTG14790.8614764506471266No Hit
CTGGATGACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTAGAT14180.8259456436900782No Hit
CTGGATGACTGGGATTAGATACCCCCGCGGCCTAGAGGAGCCTGTTCTAG12980.7560489742663762No Hit
CTGGATGACTGGGATTAGATACCCCTCGTCGGCAGCGTCAGATGTGTATA12930.7531366130403886No Hit
CTGGATGACTGGGATTAGATACCCCCTACCTAGAGGAGCCTGTTCTAGAT11600.6756678044291189No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGAT11440.6663482485059588No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT11400.6640183595251686No Hit
CTGGATGACTGGGATTAGATACCCCGAGAGGAGCCTGTTCTAGATTGAGA11310.6587761093183909No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA9810.5714052725387635No Hit
CTGGATGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG9550.5562609941636281No Hit
CTGGATGACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGT9540.5556785219184306No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAC9420.5486888549760603No Hit
CTGGATGACTGGGATTAGATACCCCCACTATGCTTAGTTCTAAACCCAAA8720.5079157978122343No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACATAGAGGAGCCTGTTCTA8250.480539602287951No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCACTAAACCTCAAC7660.4461737398212975No Hit
CTGGATGACTGGGATTAGATACCCCTGACGCGACGACTAGAGGAGCCTGT6750.3931687655083236No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAAT6390.37219976468121296No Hit
CTGGATGACTGGGATTAGATACCCCCTCAATCTAGAGGAGCCTGTTCTAG5890.3430761524213371No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA5260.30638040097389363No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAAC4820.2807516221852029No Hit
CTGGATGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA4000.23298889807900655No Hit
CTGGATGACTGGGATTAGATACCCCTGCATCTAGAGGAGCCTGTTCTAGA3930.22891159236262393No Hit
CTGGATGACTGGGATTAGATACCCCGTCTCGTGGTCTAGAGGAGCCTGTT3820.22250439766545124No Hit
CTGGATGACTGGGATTAGATACCCCTCAATCTAGAGGAGCCTGTTCTAGA3680.214349786232686No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT3660.21318484174229096No Hit
CTGGATGACTGGGATTAGATACCCCAGTCTCGTGGTCTCTAGAGGAGCCT3620.21085495276150093No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT3320.19338078540557543No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT3070.17881897927563753No Hit
CTGGATGACTGGGATTAGATACCCCATCCGGGAAACTGCAGGGGAAGCTA3070.17881897927563753No Hit
CTGGATGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACA2930.17066436784287228No Hit
CTGGATGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT2760.1607623396745145No Hit
CTGGATGACTGGGATTAGATACCTCGTCGGCAGCGTCAGAGGAGCCTGTT2760.1607623396745145No Hit
CTGGATGACTGGGATTAGATACCCCATTATTCTCTTTTTTAATTTTTTTT2390.1392108666022064No Hit
CTGGATGACTGGGATTAGATACCCCTCAGGCTCCTCTAGAGGAGCCTGTT2160.12581400496266354No Hit
CTGGATGACTGGGATTAGATACCCCAGAGGAGCCTGTTCTAGATTGAGAC2020.1176593935298983No Hit
CTGGATGACTGGGATTAGATACCCTCTCGTGGGCTAGAGGAGCCTGTTCT1980.11532950454910824No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT1940.11299961556831817No Hit
CTGGATGACTGGGATTAGATACCCCGAGGAGCCTGTTCTAGATTGAGACT1800.10484500413555295No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT206.170376E-8294.9938
CTGGGTT255.293259E-10294.9939
CTGGATC108.4593665E-4294.9931
ACTGGGT255.293259E-10294.9938
CTGGATG170400.0294.733341
GACTGGG167750.0294.28967
TGACTGG169100.0294.120766
TGGATGA170400.0293.954282
GGATGAC170250.0293.52023
ATGACTG169900.0293.256745
ACTGGGA168300.0293.240238
GATGACT170200.0292.999824
CTGGGAT168500.0292.979749
GGATACT650.0249.609483
TACTGGG650.0249.609486
GCTGGGA251.877761E-7235.99447
GACGGGA251.877761E-7235.99447
CTGGATT1550.0228.38179
ACTGGAT1550.0228.38178
CTAGGAT202.2789662E-5221.244779
Produced by FastQC (version 0.11.5)