FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-207-6-5-7-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-207-6-5-7-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170050
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC7868146.26933254925022No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT69074.061746545133784No Hit
CTGGATGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACAGATCTG61473.6148191708321087No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT59653.507791825933549No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT58973.4678035871802413No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC48182.8332843281387827No Hit
CTGGATGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACAGATCT29731.7483093207880034No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT29611.741252572772714No Hit
CTGGATGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT25491.498970890914437No Hit
CTGGATGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG24671.4507497794766244No Hit
CTGGATGACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTACAG24381.433695971773008No Hit
CTGGATGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT22961.3501911202587475No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT20101.1820052925610114No Hit
CTGGATGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACAGAT19291.1343722434578065No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT19141.1255513084386946No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC18421.0832108203469568No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA18161.0679211996471627No Hit
CTGGATGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACAGA17621.0361658335783592No Hit
CTGGATGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTACAGA15090.8873860629226697No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT13080.7691855336665687No Hit
CTGGATGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC11910.7003822405174949No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT11300.6645104381064393No Hit
CTGGATGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACAGAT11200.6586298147603646No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATAT10720.6304028226992061No Hit
CTGGATGACTGGGATTAGATACCCCTGCATCTAGAGGAGCCTGTTCTACA8830.5192590414583945No Hit
CTGGATGACTGGGATTAGATACCCTAGAGGAGCCTGTTCTACAGATCTGT8830.5192590414583945No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAGAT8710.512202293443105No Hit
CTGGATGACTGGGATTAGATACCCCTCTCTAGAGGAGCCTGTTCTACAGA8580.5045574830932079No Hit
CTGGATGACTGGGATTAGATACCCCGTCTCGTGTAGAGGAGCCTGTTCTA8120.4775066157012643No Hit
CTGGATGACTGGGATTAGATACCCCTCCTAGAGGAGCCTGTTCTACAGAT7420.4363422522787415No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAAC6600.38812114084092914No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT5850.344016465745369No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGAT5310.3122610996765657No Hit
CTGGATGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA4910.288738606292267No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT4500.26462805057336075No Hit
CTGGATGACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGT3940.23169655983534257No Hit
CTGGATGACTGGGATTAGATACCCCTCTCGAGGAGCCTGTTCTACAGATC3110.18288738606292268No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGAT3050.17935901205527785No Hit
CTGGATGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACA2950.17347838870920318No Hit
CTGGATGACTGGGATTAGATACCCAACTATGCTTAGCCCTAAACCTCAAC2450.14407527197882974No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAAC2260.13290208762128786No Hit
CTGGATGACTGGGATTAGATACCCCGTCTCGTGGTCTCTAGAGGAGCCTG2090.12290502793296089No Hit
CTGGATACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA1840.10820346956777419No Hit
CTGGATGACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA1800.1058512202293443No Hit
CTGGATGACTGGGATTGATACCCCACTATGCTTAGCCCTAAACCTCAACA1800.1058512202293443No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAAC1710.1005586592178771No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGGG350.0294.987956
GATGGCT108.4597315E-4294.987954
ACGGATT108.4597315E-4294.987958
GACGGGA350.0294.987957
CTGGGTT305.456968E-12294.987959
CTGGGGT108.4597315E-4294.987959
CTGGGAG108.4597315E-4294.987959
GGCTGGG108.4597315E-4294.987957
ACTGGGT350.0294.987958
GGATGAC167800.0294.900023
CTGGATG168850.0294.813231
TGACTGG166900.0294.81126
TGGATGA168500.0294.637822
ATGACTG167350.0294.3715
GATGACT167650.0294.284154
GACTGGG166350.0294.278637
ACTGGGA166700.0293.306828
CTGGGAT166500.0293.304849
ATGACGG500.0265.489145
CTGGATT1000.0265.489149
Produced by FastQC (version 0.11.5)