FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-206-6-8-1-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-206-6-8-1-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173616
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG7444642.879688507971615No Hit
GGTACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGGAATCGCTG96475.556515528522716No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG76744.420099529997235No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG49472.8493917611280066No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG38542.219841489263662No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC36392.0960049764998616No Hit
GGTACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT32581.8765551562068012No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG28791.658257303474334No Hit
GGTACACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATAT27891.606418763247627No Hit
GGTACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG27211.5672518661874482No Hit
GGTACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG22431.2919316192056032No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG20901.203806100820201No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG20201.1634872361994286No Hit
GGTACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAGG17391.0016357939360427No Hit
GGTACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG17340.998755875034559No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG16950.9762925076029858No Hit
GGTACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGGAATCGC14790.8518800110588886No Hit
GGTACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGGAATCGCT14170.8161690166804902No Hit
GGTACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT13010.7493548981660676No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG10370.5972951801677265No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGATGC9340.5379688507971616No Hit
GGTACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAGGA8790.5062897428808405No Hit
GGTACACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTAGGAAT8600.4953460510552023No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATTA8520.4907381808128283No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG7540.43429177034374716No Hit
GGTACACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTAGGAATCG7170.41298037047276753No Hit
GGTACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT5650.32543083586766197No Hit
GGTACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGGAATCG5420.3121832089208368No Hit
GGTACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT5130.2954796792922311No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATAG4840.27877614966362546No Hit
GGTACACTGGGATTAGATACCCCCCCGTCCTAGAGGAGCCTGTTCTAGGA4770.27474426320154827No Hit
GGTACACTGGGATTAGATACCCCCCCTAGAGGAGCCTGTTCTAGGAATCG4620.26610450649709705No Hit
GGTACACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTAGGAATCGC4590.2643765551562068No Hit
GGTACACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAATAG4420.2545848308911621No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATAG4300.24767302552760115No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACAG4270.2459450741867109No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGACAG4250.24479310662611742No Hit
GGTACACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATAA4160.23960925260344668No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGATAC4100.23615334992166623No Hit
GGTACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGGAATCG4030.232121463459589No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAACAG3990.22981752833840197No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATAG3960.22808957699751173No Hit
GGTACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGGAATC3570.20562620956593863No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTACCCCTAAACTCTAATAG3420.19698645286148742No Hit
GGTACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT3280.18892267993733297No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAGATAG3270.18834669615703623No Hit
GGTACACTGGGATTAGATACCCCACGAGACAGAGGAGCCTGTTCTAGGAA3230.1860427610358492No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAGTAG3220.1854667772555525No Hit
GGTACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGGAATCG3110.17913095567228826No Hit
GGTACACTGGGATTAGATACCCCTCTATACTAAATTACTTTATCCTATTA2920.1681872638466501No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAACAG2800.1612754584830891No Hit
GGTACACTGGGATTAGATACCCCACGAGACGTCTAGAGGAGCCTGTTCTA2750.15839553958160538No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG2680.15436365311952815No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT2580.14860381531656067No Hit
GGTACACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGCC2560.1474518477559672No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAGG2470.1422679937332965No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACAG2410.13881209105151598No Hit
GGTACACTGGGATTAGATACCCCCTCGTCGGCAGCGTCAGATGTGTAGAG2380.13708413971062575No Hit
GGTACACTGGGATTAGATACCCCGCACTAGAGGAGCCTGTTCTAGGAATC2350.1353561883697355No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATCGATTG2320.13362823702884527No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAG2310.13305225324854852No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTTGGTAA2270.13074831812736154No Hit
GGTACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTAG2200.12671643166528432No Hit
GGTACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAGCTCTAATAG2190.12614044788498757No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTAACAG2110.12153257764261358No Hit
GGTACACTGGGATTAGATACCCCGTCTCGTAGAGGAGCCTGTTCTAGGAA2080.11980462630172334No Hit
GGTACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGGAATC2070.1192286425214266No Hit
GGTACACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT2050.11807667496083311No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAG2050.11807667496083311No Hit
GGTACACTGGGATTAGATACCCCTGACGCTAAGAGGAGCCTGTTCTAGGA2000.11519675605934937No Hit
GGTACACTGGGATTAGATACCCCACTACGCTTAGCCCTAAACCTCAACAG1960.11289282093816237No Hit
GGTACACTGGGATTAGATACCCCGTCTCTCTAGAGGAGCCTGTTCTAGGA1910.11001290203667864No Hit
GGTACATGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT1880.10828495069578839No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAGCCCTAAACCTCAACAG1790.10310109667311769No Hit
GGTACACTGGGATTAGATACCCCTCGTCGGCAGCGTCAGATGTGTATAGA1770.1019491291125242No Hit
GGTACACTGGGATTAGATACCCCGTCTCGTCTAGAGGAGCCTGTTCTAGG1760.10137314533222744No Hit
GGTACACTGGGATTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT1740.10022117777163395No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACCT206.170194E-8294.9981
ACCGGGA108.459021E-4294.9986
CCGGGAT108.459021E-4294.9987
TTGGATT206.170194E-8294.9988
ACACCGG108.459021E-4294.9984
TGGGTTA305.456968E-12294.9988
CTTGGAT206.170194E-8294.9987
GGGGTTA157.229999E-6294.9989
TGGGGTT157.229999E-6294.9988
CTGGGTT305.456968E-12294.9987
CTGGGGT157.229999E-6294.9987
CACCGGG108.459021E-4294.9985
ACTGGGG157.229999E-6294.9986
GGTACAC172600.0294.74161
CACTGGG170350.0294.738225
ACTGGGA170000.0294.477426
ACACTGG171800.0294.3974
CTGGGAT169850.0294.39017
TGGGATT169700.0293.346568
GTACACT172950.0293.121732
Produced by FastQC (version 0.11.5)