FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-205-6-5-7-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-205-6-5-7-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169058
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC6628839.21021187994653No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT93345.521182079523004No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT73604.353535473032923No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT62623.704054229909262No Hit
CTGGATGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTATAAGCACT57323.3905523548131407No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC42232.4979592802470156No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA38512.277916454707852No Hit
CTGGATGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTATAAGCAC27461.6242946207810336No Hit
CTGGATGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC26321.5568621419867736No Hit
CTGGATGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT25851.5290610323084386No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT23871.4119414638763028No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC23371.382365815282329No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT23081.3652119390978243No Hit
CTGGATGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTATAAGC21461.2693868376533497No Hit
CTGGATGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT20441.2090525145216433No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT17131.0132617208295378No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT15600.9227602361319784No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCT14450.8547362443658388No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAAC12570.743531805652498No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAAT11580.6849720214364301No Hit
CTGGATGACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTATAAGCA10070.5956535626826296No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGAT9470.5601627843698612No Hit
CTGGATGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT9410.5566137065385844No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGAT9390.5554306805948255No Hit
CTGGATGACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAAT8660.5122502336476239No Hit
CTGGATGACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTATAAGC8420.49805392232251655No Hit
CTGGATGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCA8250.48799820180056547No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTATAAGCA7910.4678867607566634No Hit
CTGGATGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTATAAGC7850.4643376829253866No Hit
CTGGATGACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTATAAGC7450.4406771640502076No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAGT7440.4400856510783282No Hit
CTGGATGACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTATAAG7390.4371280862189308No Hit
CTGGATGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC7110.4205657230063055No Hit
CTGGATGACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTATAAGCA7080.4187911840906671No Hit
CTGGATGACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTATAAGCA6540.38684948360917554No Hit
CTGGATGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTATAAG6360.376202250115345No Hit
CTGGATGACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTATAA5920.3501756793526482No Hit
CTGGATGACTGGGATTAGATACCCCGACGACCTAGAGGAGCCTGTTCTAT5780.34189449774633557No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA4440.26263175951448614No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTTGAT4370.2584911687113298No Hit
CTGGATGACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGT4340.2567166297956914No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCGTAAACATTGAT4320.25553360385193247No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAGCCCTAAACCTCAAC3990.23601367577990987No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAAT3900.2306900590329946No Hit
CTGGATGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA3890.23009854606111513No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAAC3850.22773249417359726No Hit
CTGGATGACTGGGATTAGATACCCCTGCTTAGAGGAGCCTGTTCTATAAG3760.22240887742668197No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTCAAC3730.22063433851104355No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT3510.20762105312969514No Hit
CTGGATGACTGGGATTAGATACCCCACTGCATGTGTCAATACGTAGGACT3360.19874835855150305No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTTGAT3330.19697381963586463No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAAT3190.188692638029552No Hit
CTGGATGACTGGGATTAGATACCCCACCATGCTTAGCCCTAAATCTCAAC3140.1857350731701546No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTATAAT3090.18277750831075726No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA3070.1815944823669983No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTATCCCTAAACTTCAAC3000.17745389156384198No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT2750.16266606726685515No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCTATAAACTCAAAT2710.16030001537933727No Hit
CTGGATGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACA2680.15852547646369883No Hit
CTGGATGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG2420.14314613919483254No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACATTGAT2310.13663949650415833No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCCTAGCCGTAAACTTTGAT2080.12303469815093046No Hit
CTGGATGACTGGGATTAGATACCCCAGAACAGAGCGGGCACTTCCAGTTC2000.11830259437589467No Hit
CTGGATGACTGGGATTAGATACCCCAGAGGAGCCTGTTCTATAAGCACTG1830.10824687385394363No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATAT1780.10528930899454626No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTAGAGGAGCCTG1750.10351477007890784No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGAT255.293259E-10295.09
GGATGCT157.229466E-6295.03
TGACCGG255.293259E-10295.06
TGGATGC206.1694664E-8294.999972
CTGGATG167950.0294.91221
TGACTGG165500.0294.732646
TGGATGA167800.0294.648382
GGATGAC167550.0294.55983
GATGACT166750.0294.115424
GACTGGG164550.0294.013987
ATGACTG166600.0293.76055
ACTGGGA164800.0293.120458
CTGGGAT164700.0292.94029
GACTGGA1300.0283.653847
ACTGGAT1150.0269.347848
TACTGGG450.0262.222236
AGACTGG400.0258.1255
CTGGGTT400.0258.1259
TGACGGG700.0252.857136
ACGGGAT700.0252.857138
Produced by FastQC (version 0.11.5)