FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-204-6-1-1-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-204-6-1-1-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169409
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG6751839.855025411873044No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG91095.3769280262559835No Hit
GGTACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGCTGAGAACT83304.917094133133423No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG55093.2518933468705913No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC35812.1138192185775253No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG33671.9874977126362825No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG32661.9278786841313036No Hit
GGTACACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATAT31381.852321895530934No Hit
GGTACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG30211.7832582684509088No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG28011.6533950380440237No Hit
GGTACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT26841.5843314109639985No Hit
GGTACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG26341.554817040416979No Hit
GGTACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG20631.2177629287700182No Hit
GGTACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT18581.0967540095272388No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG16240.9586267553671883No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG16200.9562656057234267No Hit
GGTACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAGC15960.9420987078608575No Hit
GGTACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGCTGAGAA13740.8110549026320916No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG13660.8063326033445685No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG11610.6853236841017892No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGATGC11200.6611219002532333No Hit
GGTACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGCTGAGAAC11160.6587607506094718No Hit
GGTACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAGCT11050.6522675890891275No Hit
GGTACACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTAGCTGA9680.5713982137902944No Hit
GGTACACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTAGCTGAGA7590.44802814490375364No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATTA7320.4320903848083632No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTACCCCTAAACTCTAATAG6830.40316630167228423No Hit
GGTACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT6440.38014509264560914No Hit
GGTACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGCTGAGA6390.37719365559090723No Hit
GGTACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGCTGAGA6150.3630267577283379No Hit
GGTACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGCTGAG5960.3518112969204706No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAGC5420.3199357767296897No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATAG5030.29691456770301455No Hit
GGTACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT4790.2827476698404453No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAACAG4570.2697613467997568No Hit
GGTACACTGGGATTAGATACCCCCCCGTCCTAGAGGAGCCTGTTCTAGCT4560.2691710593888164No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGATAC4340.2561847363481279No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACAG4300.2538235867043664No Hit
GGTACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTAG4290.253233299293426No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATAG4260.2514624370606048No Hit
GGTACACTGGGATTAGATACCCCTCTATACTAAATTACTTTATCCTATTA4160.24555956295120093No Hit
GGTACACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATAA4100.2420178384855586No Hit
GGTACACTGGGATTAGATACCCCACGAGACAGAGGAGCCTGTTCTAGCTG3870.22844122803392974No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAGTAG3730.22017720428076432No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAG3510.2071908812400758No Hit
GGTACACTGGGATTAGATACCCCCCCTAGAGGAGCCTGTTCTAGCTGAGA3390.20010743230879116No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAGATAG3300.19479484561032767No Hit
GGTACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT3280.1936142707884469No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCGTAAACCCAGACGT3070.18121823515869878No Hit
GGTACACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTAGCTGAGAA2990.17649593587117568No Hit
GGTACACTGGGATTAGATACCCCCTCGTCGGCAGCGTCAGATGTGTAGAG2970.1753153610492949No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACAG2930.17295421140553335No Hit
GGTACACTGGGATTAGATACCCCGTCTCGTCTAGAGGAGCCTGTTCTAGC2830.16705133729612948No Hit
GGTACACTGGGATTAGATACCCCACGAGACGTCTAGAGGAGCCTGTTCTA2750.16232903800860637No Hit
GGTACACTGGGATTAGATACCCCGCACTAGAGGAGCCTGTTCTAGCTGAG2680.1581970261320237No Hit
GGTACACTGGGATTAGATACCCCTATAAGAGACAGTCTCAATCTAGAGGA2480.14639127791321596No Hit
GGTACACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATAA2380.14048840380381208No Hit
GGTACACTGGGATTAGATACCCCTCGTCGGCAGCGTCAGATGTGTATAGA2320.13694667933816976No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAACAG2250.13281466746158704No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATAG2240.13222438005064666No Hit
GGTACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAGCTCTAATAG2200.1298632304068851No Hit
GGTACACTGGGATTAGATACCCCAAGAGTGCTGGCGGAATTCTTCTAAGT2200.1298632304068851No Hit
GGTACACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT2010.11864776959901777No Hit
GGTACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT1990.11746719477713698No Hit
GGTACACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAATAG1970.11628661995525622No Hit
GGTACACTGGGATTAGATACCCCACCATGCTTAGCCCTAAACCTCAACAG1950.11510604513337544No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGATGA1920.11333518290055428No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACGG1870.11038374584585234No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG1860.10979345843491195No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAGCCCTAAACCTCAACAG1760.10389058432550809No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGACAG1760.10389058432550809No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAGACCTCAACAG1720.10152943468174655No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAACAG1700.10034885985986577No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACAT400.0294.993841
ACCGGGA108.4592E-4294.993846
CCGGGAT108.4592E-4294.993847
TGGGTTA206.170012E-8294.993848
GGGGTTA108.4592E-4294.993849
TACTGGG108.4592E-4294.993843
CTGGGTT206.170012E-8294.993847
CTGGGGT108.4592E-4294.993847
CACCGGG108.4592E-4294.993845
ACTGGGT206.170012E-8294.993846
CACTGGG166450.0294.99385
ACGGGAT500.0294.99386
CTGGATT900.0294.99387
ACTGGGG157.230099E-6294.99386
ACTGGGA166250.0294.727636
CTGGGAT166300.0294.550327
GGTACAC169100.0294.470431
ACACTGG167950.0294.46694
TGGGATT165850.0293.92668
TACACTG168600.0293.85653
Produced by FastQC (version 0.11.5)