FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-203-6-1-2-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-203-6-1-2-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163400
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA6408639.2203182374541No Hit
CAACACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGGAATCGCT82965.077111383108935No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA73874.520807833537332No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA58323.569155446756426No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA39882.440636474908201No Hit
CAACACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT33022.0208078335373316No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA29681.8164014687882497No Hit
CAACACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA27681.6940024479804163No Hit
CAACACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA25681.5716034271725827No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA24011.4694002447980417No Hit
CAACACACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATA23851.459608323133415No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA22971.4057527539779682No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA21501.3157894736842104No Hit
CAACACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA19731.2074663402692778No Hit
CAACACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA17291.058139534883721No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA16961.0379436964504285No Hit
CAACACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGGAATCGC16451.0067319461444308No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA13470.8243574051407588No Hit
CAACACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAG13410.8206854345165238No Hit
CAACACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGGAATCG11540.7062423500611995No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGATG9600.587515299877601No Hit
CAACACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAGG8600.5263157894736842No Hit
CAACACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT8600.5263157894736842No Hit
CAACACACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTAGGAA8260.5055079559363524No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA8020.49082007343941253No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT7800.4773561811505508No Hit
CAACACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGGAAT7760.47490820073439416No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATA6600.4039167686658507No Hit
CAACACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGGAATC6480.3965728274173807No Hit
CAACACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGGAATC6150.3763769889840881No Hit
CAACACACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATA5790.3543451652386781No Hit
CAACACGCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA5430.332313341493268No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGATA4810.29436964504283963No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAGATA4530.277233782129743No Hit
CAACACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG4520.2766217870257038No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAG4250.26009791921664627No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATA4190.2564259485924113No Hit
CAACACACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTAGGAATC3710.2270501835985312No Hit
CAACACACTGGGATTAGACACCCCACTATGCTTAGCCCTAAACCTCAACA3580.21909424724602203No Hit
CAACACACTGGGATTAGATACCCCGTCTCGTCTAGAGGAGCCTGTTCTAG3460.21175030599755204No Hit
CAACACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAGCTCTAATA3430.20991432068543453No Hit
CAACACACTGGGATTAGATACCCCTCTATACTAAATTACTTTATCCTATT3410.20869033047735616No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA3390.20746634026927785No Hit
CAACACACTGGGATTAGATACCCCACGAGACAGAGGAGCCTGTTCTAGGA3250.1988984088127295No Hit
CAACACACTGGGATTAGATACCCCCTATGCTTAGCCCTAAACTCTAATAG3130.1915544675642595No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAACA3050.18665850673194614No Hit
CAACACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTA2960.18115055079559364No Hit
CAACACACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAATA2850.1744186046511628No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA2820.17258261933904528No Hit
CAACACACTGGGATTAGATACCCCACGAGACGTCTAGAGGAGCCTGTTCT2800.17135862913096694No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA2690.1646266829865361No Hit
CAACACACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTAGGAATCG2480.15177478580171358No Hit
CAACACACTGGGATTAGATACCCCCCCGTCCTAGAGGAGCCTGTTCTAGG2460.15055079559363527No Hit
CAACACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG2400.14687882496940025No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAGTA2350.1438188494492044No Hit
CAACACACTGGGATTAGATACCCCGTCTCTCTAGAGGAGCCTGTTCTAGG2310.14137086903304774No Hit
CAACACACTGGGATTAGATACCCCCCCTAGAGGAGCCTGTTCTAGGAATC2260.1383108935128519No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAACA2260.1383108935128519No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTACCCCTAAACTCTAATA2150.13157894736842105No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATCGATT2150.13157894736842105No Hit
CAACACACTGGGATTAGATACCCCAATACGCTTAGCCCTAAACTCCAATA2140.1309669522643819No Hit
CAACACACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATA2130.1303549571603427No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACA2130.1303549571603427No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAGATA2070.1266829865361077No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAACA2060.12607099143206854No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACA2020.12362301101591186No Hit
CAACACACTGGGATTAGATACCCCGTCTCGTCGTCGTCGTCGTCTAGAGG1980.1211750305997552No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA1950.1193390452876377No Hit
CAACACCCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA1890.1156670746634027No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAACA1820.11138310893512852No Hit
CAACACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGGAATC1820.11138310893512852No Hit
CAACACACTGGGATTAGATACCCCACGAGAGGAGCCTGTTCTAGGAATCG1730.10587515299877602No Hit
CAACACACTGGGATTAGATACCCCATTATTCTCTTTTTTAATTTTTTTTT1730.10587515299877602No Hit
CAACACACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1700.1040391676866585No Hit
CAACACACTGGGATTAGATACCCCTGGCGAGAGACTGTCTCTAGAGGAGC1700.1040391676866585No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGG1550.0294.975836
ACGCTGG1550.0294.975835
CTGGGTT350.0294.975838
TGGGATA550.0294.975839
CCCTGGG450.0294.975836
ACACGGG305.456968E-12294.97585
TTGGATT108.4604725E-4294.97589
TGGGTTA305.456968E-12294.97589
GTGGGAT206.171285E-8294.97588
CAGTGGG206.171285E-8294.97586
CACTGGG157150.0294.600436
ACACTGG159150.0294.512485
ACTGGGA157350.0293.663577
CTGGGAT159450.0293.403388
TGGGATT159300.0292.938969
CACACTG160750.0291.948064
ACTGGAT1850.0287.00357
GCTGGGA1600.0285.75787
ACACACT168000.0279.875853
CACTGGA1950.0279.848886
Produced by FastQC (version 0.11.5)