FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-202-6-1-2-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-202-6-1-2-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162538
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA6769141.646261182000515No Hit
CAACACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACAGATCTGT71174.378668372934329No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA68194.195326631310832No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA45212.7815034022813125No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA40112.467730622992777No Hit
CAACACACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATA34322.111506232388734No Hit
CAACACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT32872.022296324551797No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA28871.7762000270705927No Hit
CAACACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA22561.3879831177939927No Hit
CAACACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA22191.3652192102769813No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA19681.2107937836075258No Hit
CAACACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA19081.1738793389853448No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA18881.1615745241112847No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA16190.9960747640551748No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA16010.9850004306685206No Hit
CAACACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACAGATCTG13620.8379578929235011No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA13430.8262683187931438No Hit
CAACACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA13290.8176549483813016No Hit
CAACACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTACAGATCT12250.7536699110361884No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA11800.725984077569553No Hit
CAACACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAC11090.6823019847666392No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGATG9980.614010262215605No Hit
CAACACACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTACAGA9130.5617147990008491No Hit
CAACACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT8980.5524861878453038No Hit
CAACACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTACA8510.5235698728912623No Hit
CAACACACTGGGATTAGATACCCCACGAGACAGAGGAGCCTGTTCTACAG8310.5112650580172021No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT6580.4048284093565812No Hit
CAACACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTACAGATC6470.3980607611758481No Hit
CAACACACTGGGATTAGATACCCCTCTATACTAAATTACTTTATCCTATT6360.39129311299511504No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGATA6300.3876016685328969No Hit
CAACACGCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA5560.34207385349887415No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA5560.34207385349887415No Hit
CAACACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACAGAT5420.33346048308703197No Hit
CAACACACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTACAGATC5410.3328452423433289No Hit
CAACACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACAGATC5260.3236166311877838No Hit
CAACACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG5170.3180794644944567No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATA4800.29531555697744527No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAGTA4360.2682449642545128No Hit
CAACACACTGGGATTAGATACCCCCCCTAGAGGAGCCTGTTCTACAGATC4010.2467115382249074No Hit
CAACACACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAATA3960.24363533450639235No Hit
CAACACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAGCTCTAATA3850.23686768632565922No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAACA3760.23133051963233212No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAGATA3630.22333238996419297No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTTGGTA3570.2196409455019749No Hit
CAACACACTGGGATTAGATACCCCCCCGTCCTAGAGGAGCCTGTTCTACA3420.21041233434642975No Hit
CAACACACTGGGATTAGATACCCCGTCTCGTCTAGAGGAGCCTGTTCTAC3410.20979709360272678No Hit
CAACACACTGGGATTAGACACCCCACTATGCTTAGCCCTAAACCTCAACA3370.20733613062791473No Hit
CAACACACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATA3290.2024142046782906No Hit
CAACACACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTACAGATCT3180.19564655649755752No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAC3070.18887890831682436No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGACA2970.18272650087979425No Hit
CAACACACTGGGATTAGATACCCCACGAGACGTCTAGAGGAGCCTGTTCT2900.1784198156738732No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTACCCCTAAACTCTAATA2820.1734978897242491No Hit
CAACACACTGGGATTAGATACCCCACGAGAGGAGCCTGTTCTACAGATCT2770.17042168600573404No Hit
CAACACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG2750.169191204518328No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATCGATT2720.167345482287219No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCGTAAACCCAGACG2650.16303879708129793No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACA2570.1581168711316738No Hit
CAACACACTGGGATTAGATACCCCTGGCGAGAGACTGTCTCTAGAGGAGC2540.1562711489005648No Hit
CAACACACTGGGATTAGATACCCCATTATTCTCTTTTTTAATTTTTTTTT2520.15504066741315875No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATA2510.15442542666945577No Hit
CAACACACTGGGATTAGATACCCCACCATGCTTAGCCCTAAACCTCAACA2450.1507339822072377No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAACA2260.13904440807688048No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAGCCCTAAACCTCAACA2250.13842916733317745No Hit
CAACACACTGGGATTAGATACCCCTATAAGAGACAGTCTCAATCTAGAGG2210.13596820435836543No Hit
CAACACCCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA2120.13043103766503833No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAACA2050.12612435245911727No Hit
CAACACACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGTT2040.12550911171541423No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAACA2000.12304814874060221No Hit
CAACACACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATA1970.12120242650949316No Hit
CAACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1880.11566525981616606No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACG1870.11505001907246305No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACTAGAGGAGCCTGT1790.11012809312283896No Hit
CAACACACTGGGATTAGATACCCCGAGTACTCCGGCTTGAGGAATGCGTC1630.10028424122359078No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGA400.0294.99116
ATGGGAT400.0294.99117
GCTGGGA1050.0294.99117
CTTGGAT108.4591174E-4294.99118
ACATGGG400.0294.99115
CTGGATT1150.0294.99118
CCCTGGG450.0294.99116
ACCGGGA157.2298244E-6294.991067
CACCGGG157.2298244E-6294.991066
ACTGGTA157.2298244E-6294.991067
CACTGGG156650.0294.802766
ACACTGG158300.0294.4325
ACTGGGA156900.0293.862987
CTGGGAT158450.0293.687878
TGGGATT158650.0292.75989
CACACTG159750.0292.682864
ACGCTGG1150.0282.16545
CGCTGGG1100.0281.58246
ACACACT167900.0278.65153
ACCCTGG500.0265.491945
Produced by FastQC (version 0.11.5)