FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-201-6-5-7-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-201-6-5-7-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155902
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC3766224.157483547356673No Hit
CTGGATGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGCTGAGAA2860718.34934766712422No Hit
CTGGATGACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGCTGAGA93095.97105874203025No Hit
CTGGATGACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGCTGAG51073.2757758078793087No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT44472.8524329386409413No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT40842.619594360559839No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT33532.150710061448859No Hit
CTGGATGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT33332.137881489653757No Hit
CTGGATGACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTAGCTGAG24671.5824043309258378No Hit
CTGGATGACTGGGATTAGATACCCCTCCTAGAGGAGCCTGTTCTAGCTGA22011.4117843260509808No Hit
CTGGATGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCA21171.357904324511552No Hit
CTGGATGACTGGGATTAGATACCCCAATATGCTAGAGGAGCCTGTTCTAG20331.3040243229721236No Hit
CTGGATGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC19201.2315428923297969No Hit
CTGGATGACTGGGATTAGATACCCCTCTCCTAGAGGAGCCTGTTCTAGCT18671.1975471770727764No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA16621.0660543161729805No Hit
CTGGATGACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTAGCTGA14170.9089043116829802No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT13400.8595143102718374No Hit
CTGGATGACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGCTG13090.8396300239894292No Hit
CTGGATGACTGGGATTAGATACCCCGAGACTAGAGGAGCCTGTTCTAGCT12180.7812600223217149No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGAT11870.7613757360393067No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT11840.7594514502700415No Hit
CTGGATGACTGGGATTAGATACCCCACGATAGAGGAGCCTGTTCTAGCTG11760.7543200215520006No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGAC11370.7293043065515517No Hit
CTGGATGACTGGGATTAGATACCCTAGAGGAGCCTGTTCTAGCTGAGAAC10520.6747828764223679No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAAC10400.6670857333453067No Hit
CTGGATGACTGGGATTAGATACCCCCTCCCCTAGAGGAGCCTGTTCTAGC10330.6625957332170209No Hit
CTGGATGACTGGGATTAGATACCCCTCTCGTGAGCTAGAGGAGCCTGTTC10160.6516914471911842No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT9980.6401457325755924No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC9690.6215443034726944No Hit
CTGGATGACTGGGATTAGATACCCCTCGTCTAGAGGAGCCTGTTCTAGCT9450.6061500173185719No Hit
CTGGATGACTGGGATTAGATACCCCTCCAGCTAGAGGAGCCTGTTCTAGC8580.550345730009878No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACAGAGGAGCCTGTTCTAGC8030.5150671575733474No Hit
CTGGATGACTGGGATTAGATACCCCGTCTCTCTCGTGGTCTCGCTAGAGG7690.49325858552167384No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT6290.4034585829559595No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT6210.39832715423791865No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT6060.38870572539159215No Hit
CTGGATGACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTAGCT5770.3701042962886942No Hit
CTGGATGACTGGGATTAGATACCCCTGTCGGAGCTAGAGGAGCCTGTTCT5760.3694628676989391No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAC5640.36176572462187784No Hit
CTGGATGACTGGGATTAGATACCCCGTGCGTAGAGGAGCCTGTTCTAGCT4850.3110928660312247No Hit
CTGGATGACTGGGATTAGATACCCTCAGGTCTCGTGTCTCTCTAGAGGAG4760.3053200087234288No Hit
CTGGATGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGCTG4750.3046785801336737No Hit
CTGGATGACTGGGATTAGATACCCCGTAGCCCCTAGAGGAGCCTGTTCTA4400.2822285794922451No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGAT3970.2546471501327757No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT3380.21680286333722468No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT3120.200125720003592No Hit
CTGGATGACTGGGATTAGATACCCCACCTACAGAGGAGCCTGTTCTAGCT3090.19820143423432668No Hit
CTGGATGACTGGGATTAGATACCCCGTCTCGTGGTCTCGTCTCGCTAGAG2840.18216571949044913No Hit
CTGGATGACTGGGATTAGATACCCCCCCCTAGAGGAGCCTGTTCTAGCTG2530.16228143320804095No Hit
CTGGATGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA2340.150094290002694No Hit
CTGGATGACTGGGATTAGATACCCCAGAGGAGCCTGTTCTAGCTGAGAAC2000.1282857179510205No Hit
CTGGATGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT1890.12123000346371438No Hit
CTGGATGACTGGGATTAGATACCCCAAAGAAATTGACGGTGTAGAGGAGC1870.11994714628420418No Hit
CTGGATGACTGGATTAGATACCCCTAGAGGAGCCTGTTCTAGCTGAGAAC1820.11674000333542867No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTAAAC1740.11160857461738785No Hit
CTGGATGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACA1640.10519428871983683No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCGG108.458024E-4294.999976
CTGGATG155150.0294.524661
GACTGGG152100.0294.515147
TGACTGG153350.0294.422886
GGATGAC154650.0294.236973
TGGATGA155150.0294.04932
GATGACT154150.0293.756074
ACTGGGA152700.0293.35798
ATGACTG154350.0293.184335
CTGGGAT152750.0293.068739
CTGGATT1200.0245.833349
TGCTGGG251.876997E-7236.06
ACGGGAT700.0231.78578
GACGGGA700.0231.78577
TGACGGG750.0216.333336
CGGGATT750.0216.333339
ACTGGAT1450.0213.620718
GACTGGA1600.0212.031237
CCGGGAT150.0028473444196.666679
GCTGGGA304.657395E-7196.666677
Produced by FastQC (version 0.11.5)