FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-200-6-5-8-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-200-6-5-8-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154718
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA4664730.149691697152235No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGA141739.160537235486498No Hit
TGATTGACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAATAGGAT124728.061117646298428No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGA91685.925619514212955No Hit
TGATTGACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAATAGGA45962.97056580359105No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGA38932.5161907470365437No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGA32392.0934862136273735No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAA26141.6895254592225857No Hit
TGATTGACACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTAAT23621.526648483046575No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAA17321.1194560426065487No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGA16821.0871391822541656No Hit
TGATTGACACTGGGATTAGATACCCCACACAGAGAGAGGAGCCTGTTCTA16321.0548223219017825No Hit
TGATTGACACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTAATAG15821.0225054615493996No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAA14410.9313719153556794No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACTCTAGAGGAGCCTGTTC14010.905518427073773No Hit
TGATTGACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAATAG13890.897762380589201No Hit
TGATTGACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTC13450.8693235434791039No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAA12540.8105068576377668No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTA12430.8033971483602425No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAA11910.7697876135937641No Hit
TGATTGACACTGGGATTAGATACCCCCCCCTAGAGGAGCCTGTTCTAATA11380.735531741620238No Hit
TGATTGACACTGGGATTAGATACCCCGACGACGAGGAGCCTGTTCTAATA11300.7303610439638568No Hit
TGATTGACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAA11060.714848950994713No Hit
TGATTGACACTGGGATTAGATACCCCTCTACCCTAGAGGAGCCTGTTCTA10380.670898020915472No Hit
TGATTGACACTGGGATTAGATACCCCCCGTGGGCTAGAGGAGCCTGTTCT10120.6540932535322328No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGA9760.630825114078517No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAA9050.5849351723781331No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACATCGA8650.5590816840962267No Hit
TGATTGACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAATAG8270.5345208702284155No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCT7930.5125454051887951No Hit
TGATTGACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCC7730.49961866104784186No Hit
TGATTGACACTGGGATTAGATACCCCTTCGTGGGCTAGAGGAGCCTGTTC7680.4963869750126036No Hit
TGATTGACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCA7470.4828138936646027No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAA7410.4789358704223167No Hit
TGATTGACACTGGGATTAGATACCCCGACGAGAGAGGAGCCTGTTCTAAT7070.4569604053826963No Hit
TGATTGACACTGGGATTAGATACCCCACTATACTTAGCCCTAAACCTCAA6910.4466190100699337No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAA6350.41042412647526466No Hit
TGATTGACACTGGGATTAGATACCCCCCCCCTAGAGGAGCCTGTTCTAAT6110.3949120335061208No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAGA6080.3929730218849778No Hit
TGATTGACACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAA5960.3852169754004059No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGG5750.371643894052405No Hit
TGATTGACACTGGGATTAGATACCGACGATAGAGGAGCCTGTTCTAATAG5660.36582685918897606No Hit
TGATTGACACTGGGATTAGATACCGTCTCTAGAGGAGCCTGTTCTAATAG5410.34966842901278455No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAA5170.3341563360436407No Hit
TGATTGACACTGGGATTAGATACCCCTCTCGTGGGCTAGAGGAGCCTGTT4920.3179979058674492No Hit
TGATTGACACTGGGATTAGATACCCCAAAGAAATTGACGGTGTAGAGGAG4820.31153453379697255No Hit
TGATTGACACTGGGATTAGATACCCCGACGAGACGAGGAGCCTGTTCTAA4690.303132150105353No Hit
TGATTGACACTGGGATTAGATACCCGTCTCTAGAGGAGCCTGTTCTAATA3510.22686435967372898No Hit
TGATTGACACTGGGATTAGATACCCCCTATTAGAGGAGCCTGTTCTAATA3370.21781563877506174No Hit
TGATTGACACTGGGATTAGATACCCCAGAGGAGCCTGTTCTAATAGGATT2610.1686940110394395No Hit
TGATTGACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC2590.16740133662534418No Hit
TGATTGACACTGGGATTAGATACCCGAGGAGCCTGTTCTAATAGGATTCT2390.15447459248439094No Hit
TGATTGACACTGGGATTAGATACCCCACCGAGCCCACGAGACTAGAGGAG2260.14607220879277139No Hit
TGATTGACACTGGGATTAGATACCCCGAGGAGCCTGTTCTAATAGGATTC1940.12538941816724622No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCACTAAACTCCAA1870.12086505771791257No Hit
TGATTGACACTGGGATTAGATACCCCGGTACCTGTCGATGAGTCTTCGGT1830.11827970888972195No Hit
TGATTGACACTGGGATTAGATACCCCACGGTCAGACAAATGGTCGTGCCA1780.11504802285448365No Hit
TGATTGACACTGGGATTAGATACCCCTCTGGGCTAGAGGAGCCTGTTCTA1690.10923098799105468No Hit
TGATTGACACTGGGATTAGATACCCCCTCGTCGGCAGCCTGTTCTAATAG1660.10729197636991171No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATCGA1650.10664563916286404No Hit
TGATTGACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAAC1560.1008286042994351No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGACCT305.456968E-12294.997744
GACTGGG108.458155E-4294.997746
CACCGGG108.458155E-4294.997748
ACTGGGT305.456968E-12294.997749
GATTGAC153400.0294.516972
CACTGGG150450.0294.311468
TTGACAC152900.0293.936584
TGATTGA155050.0293.380521
ACTGGGA151250.0293.339879
TGACACT152850.0293.164285
GACACTG152850.0292.681766
ACACTGG152700.0292.58297
ATTGACA153950.0292.506683
TACTGGG600.0270.414588
ACTGGAT1450.0254.30849
GACACGG351.2732926E-11252.85526
ACCTGGG301.5752448E-9245.831457
CACTGGA1500.0245.831458
ATACTGG750.0235.99827
ATTGACC403.45608E-11221.24833
Produced by FastQC (version 0.11.5)