FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-20-5-8-2-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-20-5-8-2-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12081
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA422935.00538034930884No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA8877.3421074414369665No Hit
CAACACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAC6985.7776674116381095No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA3973.286151808625114No Hit
CAACACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACAGATCTGT3843.178544822448473No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA3562.946775929144938No Hit
CAACACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACAGATC3382.797781640592666No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAC1941.605827332174489No Hit
CAACACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT1881.5561625693237315No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA1871.5478851088486052No Hit
CAACACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACAGATCTG1431.183676847943051No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG1241.0264050989156526No Hit
CAACACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACAGAT1080.893965731313633No Hit
CAACACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTACAGATCT1080.893965731313633No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA1040.860855889413128No Hit
CAACACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA1040.860855889413128No Hit
CAACACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTACAGAT1020.8443009684628756No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA990.8194685870374968No Hit
CAACACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA910.753248903236487No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA840.6953066799106034No Hit
CAACACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACAGATC780.6456419170598461No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAC750.6208095356344674No Hit
CAACACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC700.5794222332588362No Hit
CAACACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA650.5380349308832051No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA640.5297574704080787No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC610.5049250889827No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA580.4800927075573214No Hit
CAACACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA560.46353778660706896No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA540.4469828656568165No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA530.43870540518169027No Hit
CAACACACTGGGATTAGATACCCCACTGCTAGAGGAGCCTGTTCTACAGA460.38076318185580665No Hit
CAACACGCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA430.3559308004304279No Hit
CAACACACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTACA430.3559308004304279No Hit
CAACACACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTC410.3393758794801755No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAC400.33109841900504927No Hit
CAACACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACAGAT400.33109841900504927No Hit
CAACACACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGC370.3062660375796705No Hit
CAACACACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTACA360.29798857710454435No Hit
CAACACACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTAC340.2814336561542919No Hit
CAACACACTGGGATTAGATACCCCGAGCCACGAGACCTAGAGGAGCCTGT310.25660127472891314No Hit
CAACACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT300.24832381425378697No Hit
CAACACACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTACAGATC280.23176889330353448No Hit
CAACACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG270.22349143282840825No Hit
CAACACACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTACAGA230.19038159092790333No Hit
CAACACACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTACAGA220.1821041304527771No Hit
CAACACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTACAGAT220.1821041304527771No Hit
CAACACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTACAG210.17382666997765084No Hit
CAACACACTGGGATTAGATACCCCATACGTGCAGGGAGGGTACCTTGAGG200.16554920950252464No Hit
CAACACACTGGGATTAGATACCCCCGAGACCTAGAGGAGCCTGTTCTACA200.16554920950252464No Hit
CAACACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG190.15727174902739838No Hit
CAACACACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATA190.15727174902739838No Hit
CAACACACTGGGATTAGACACCCCACTATGCTTAGCCCTAAACCTCAACA180.14899428855227217No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA180.14899428855227217No Hit
CAACACACTGGGATTAGATACCCCCCGAAATGGTCTAGAGGAGCCTGTTC170.14071682807714594No Hit
CAACACACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTACA160.1324393676020197No Hit
CAACACACTGGGATTAGATACCCCTCCGAGCCCACGAGTCTAGAGGAGCC160.1324393676020197No Hit
CAACACACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAC160.1324393676020197No Hit
CAACACACTGGGATTAGATACCCCGAGTAGTCCTAGAGGAGCCTGTTCTA160.1324393676020197No Hit
CAACACACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCT150.12416190712689348No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAACA150.12416190712689348No Hit
CAACACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTACAGATC150.12416190712689348No Hit
CAACACACTGGGATTAGATACCCCCGCAACCTAGAGGAGCCTGTTCTACA150.12416190712689348No Hit
CAACACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTACAGATC150.12416190712689348No Hit
CAACACACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTACA150.12416190712689348No Hit
CAACACACTGGGATTAGATACCCCGAGATCTAGAGGAGCCTGTTCTACAG140.11588444665176724No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA140.11588444665176724No Hit
CAACACACTGGGATTAGATACCCCACGATACCTAGAGGAGCCTGTTCTAC140.11588444665176724No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTCAACA140.11588444665176724No Hit
CAACACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA130.107606986176641No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGG108.3615584E-4295.06
CTGGGAT11750.0295.08
GCTGGGA108.3615584E-4295.07
CACTGGG11700.0295.06
ACTGGGA11650.0295.07
ACACTGG11800.0293.755
TGGGATT11800.0293.759
CACACTG12100.0287.685944
ACACACT12650.0276.343873
AACACAC14600.0240.44522
CAACACA14800.0238.192571
ACGCTGG150.0028149525196.666665
CACGCTG150.0028149525196.666664
AACACGC150.0028149525196.666662
ACCATTT257.744868E-659.00000445-49
TCCTAGA257.744868E-659.00000430-34
CCTGTAG257.744868E-659.00000420-24
TTACATC257.744868E-659.000004120-124
CGCCAGA5500.059.00000470-74
CCTGAGC257.744868E-659.00000450-54
Produced by FastQC (version 0.11.5)