FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-2-6-4-7-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-2-6-4-7-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10737
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC371334.58135419577163No Hit
CTGGATGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC6295.858247182639471No Hit
CTGGATGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG6255.820992828536835No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT5104.749930148086057No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT4404.0979789512899325No Hit
CTGGATGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCA4053.7720033528918693No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC4043.7626897643662103No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA3393.1573065101983793No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGAC3283.054857036416131No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT3052.8406445003259755No Hit
CTGGATGACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACT2802.6078047871845023No Hit
CTGGATGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGATTGAGA2492.3190835428890755No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT2372.207320480581168No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCGTAAACATTGAT2242.086243829747602No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAG2222.067616652696284No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACAGAGGAGCCTGTTCTAGA2182.0303622985936483No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT1831.7043867001955852No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAGCCCTAAACCTCAAC1171.0896898575020955No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTA1161.0803762689764367No Hit
CTGGATGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGATT1121.043121914873801No Hit
CTGGATGACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTAGATTGA850.7916550246810097No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT750.6985191394244202No Hit
CTGGATGACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGATTG690.6426376082704666No Hit
CTGGATGACTGGGATTAGATACCCCACTATGTCTAGCCGTAAACCTTGAT250.2328397131414734No Hit
CTGGATGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA230.21421253609015556No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACTTCAAC160.149017416410543No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGACTG10500.0295.05
TGACTGG10500.0295.06
GGATGAC10600.0293.60853
CTGGATG10600.0293.60851
TGGATGA10600.0293.60852
CTGGGAT10550.0293.60199
GATGACT10550.0293.60194
GACTGGG10550.0293.60197
ACTGGGA10550.0293.60198
GCATGCA200.00664534147.5295
GCCCCAA452.5465852E-1159.000004120-124
AATCTTG452.5465852E-1159.00000440-44
ATCTTGA452.5465852E-1159.00000440-44
AAATCTT452.5465852E-1159.00000440-44
CTTTACC452.5465852E-1159.00000455-59
TTACTTT452.5465852E-1159.00000450-54
TTACCGA452.5465852E-1159.00000455-59
ATTAGAT10550.059.00000410-14
CCAAACC452.5465852E-1159.000004120-124
CCTAAAT452.5465852E-1159.00000435-39
Produced by FastQC (version 0.11.5)