FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-199-6-1-2-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-199-6-1-2-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143104
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA5526438.61806797853309No Hit
CAACACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGATTGAGAC82275.748965787119857No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA65514.57778957960644No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA38852.7148088103756707No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA33042.3088103756708405No Hit
CAACACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT30082.1019677996422184No Hit
CAACACACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATA22501.5722830948121647No Hit
CAACACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA22121.5457289803220036No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA21661.5135845259391771No Hit
CAACACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA21321.489825581395349No Hit
CAACACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAG19791.3829103309481217No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA17421.217296511627907No Hit
CAACACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA17271.206814624329159No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGATG16721.168381037567084No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA15301.0691525044722718No Hit
CAACACACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTAGATT13650.9538517441860465No Hit
CAACACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGATTGAG12880.9000447227191413No Hit
CAACACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGATTGAGA11260.7868403398926656No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA10740.7505031305903399No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA9830.6869130143112702No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA9230.6449854651162791No Hit
CAACACACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTAGATTGA8750.6114434257602862No Hit
CAACACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA8030.5611303667262969No Hit
CAACACACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATA7350.5136124776386405No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA7240.5059257602862254No Hit
CAACACACTGGGATTAGATACCCCACGAGACAGAGGAGCCTGTTCTAGAT5840.4080948121645796No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT5780.40390205724508044No Hit
CAACACACTGGGATTAGATACCCCCCCGTCCTAGAGGAGCCTGTTCTAGA5640.3941189624329159No Hit
CAACACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT4960.3466010733452594No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGATA4670.32633609123434704No Hit
CAACACGCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA4620.3228421288014311No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAGTA4570.3193481663685152No Hit
CAACACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGATTG4530.3165529964221825No Hit
CAACACACTGGGATTAGATACCCCGTCTCTCTAGAGGAGCCTGTTCTAGA4230.29558922182468694No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTACCCCTAAACTCTAATA4230.29558922182468694No Hit
CAACACACTGGGATTAGATACCCCGCACTAGAGGAGCCTGTTCTAGATTG4150.28999888193202145No Hit
CAACACACTGGGATTAGATACCCCTGACGCTAAGAGGAGCCTGTTCTAGA4090.2858061270125224No Hit
CAACACACTGGGATTAGATACCCCGTCTCGTCTAGAGGAGCCTGTTCTAG3950.2760230322003578No Hit
CAACACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG3910.27322786225402507No Hit
CAACACACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATA3760.2627459749552773No Hit
CAACACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAGA3590.25086650268336314No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA3550.24807133273703044No Hit
CAACACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC3540.24737254025044725No Hit
CAACACACTGGGATTAGATACCCCTGGCGAGAGACTGTCTCTAGAGGAGC3490.2438785778175313No Hit
CAACACACTGGGATTAGATACCCCACGAGAGGAGCCTGTTCTAGATTGAG3330.23269789803220037No Hit
CAACACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGATTGA3250.2271075581395349No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATA3010.21033653846153846No Hit
CAACACACTGGGATTAGACACCCCACTATGCTTAGCCCTAAACCTCAACA2770.19356551878354206No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGACA2680.18727638640429337No Hit
CAACACACTGGGATTAGATACCCCGTCTCGTAGAGGAGCCTGTTCTAGAT2670.1865775939177102No Hit
CAACACACTGGGATTAGATACCCCACTATACCTGGCCCTAAATCTTGATA2600.1816860465116279No Hit
CAACACACTGGGATTAGATACCCCCCCTAGAGGAGCCTGTTCTAGATTGA2580.18028846153846154No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACA2560.17889087656529518No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA2540.1774932915921288No Hit
CAACACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGATTGA2500.17469812164579607No Hit
CAACACACTGGGATTAGATACCCCACGAGACGTCTAGAGGAGCCTGTTCT2410.1684089892665474No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA2410.1684089892665474No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATCGATT2400.1677101967799642No Hit
CAACACACTGGGATTAGATACCCCTCTATACTAAATTACTTTATCCTATT2350.1642162343470483No Hit
CAACACACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAATA2300.16072227191413238No Hit
CAACACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG2280.15932468694096602No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAACA2090.1460476296958855No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATA2090.1460476296958855No Hit
CAACACACTGGGATTAGATACCCCAATACGCTTAGCCCTAAACTCCAATA2080.14534883720930233No Hit
CAACACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTA2060.14395125223613597No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCGTAAACCCAGACG2030.1418548747763864No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAGATA2030.1418548747763864No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCCAAACCTCAACA1910.1334693649373882No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAACA1870.13067419499105545No Hit
CAACACACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGC1830.1278790250447227No Hit
CAACACACTGGGATTAGATACCCCCGAGAGGAGCCTGTTCTAGATTGAGA1780.12438506261180679No Hit
CAACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1760.12298747763864043No Hit
CAACACACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTA1740.12158989266547406No Hit
CAACACACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTAGATTGAG1620.11320438282647585No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACA1610.11250559033989266No Hit
CAACACACTGGGATTAGATACCCCATTATGCCCCGCCGTAAACTTAGATA1600.11180679785330948No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG1590.11110800536672628No Hit
CAACACACTGGGATTAGATACCCCATTATTCTCTTTTTTAATTTTTTTTT1580.11040921288014312No Hit
CAACACCCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA1540.10761404293381037No Hit
CAACACACTGGGATTAGATACCCCGTACTAGGTGGAACTTCACAAAGAAG1460.1020237030411449No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGA255.293259E-10294.99236
ACAGTGG157.2279017E-6294.99235
GCTGGGA1100.0294.99237
CGCTGGG1150.0294.99236
ACGGGAT305.456968E-12294.99237
CGGGATT305.456968E-12294.99238
ACATGGG255.293259E-10294.99235
ACTGGGT255.293259E-10294.99237
ACTGGGG108.457959E-4294.99237
CCCTGGG255.293259E-10294.99236
CACTGGG138650.0294.885936
ACACTGG139600.0294.147065
ACTGGGA138950.0293.612347
CTGGGAT140450.0293.2078
TGGGATT140000.0292.885229
CACACTG140900.0291.956574
ACACACT147750.0278.720343
CACGCTG1250.0271.392944
ACGCTGG1250.0271.392945
ACACGCT1300.0260.95473
Produced by FastQC (version 0.11.5)