FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-198-6-5-7-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-198-6-5-7-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134674
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC5295539.32087856601868No Hit
CTGGATGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGACGTGGC64194.766324606085807No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT59924.449262663914341No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT53373.9629030102321163No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT48353.590151031379479No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC41373.071862423333383No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAAC33062.45481681690601No Hit
CTGGATGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC30072.2327992040037423No Hit
CTGGATGACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGACGT26651.9788526367376034No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT24021.7835662414422977No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGAT22221.6499101534074876No Hit
CTGGATGACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTA21521.597932785838395No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC19951.481354975719144No Hit
CTGGATGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGACG17981.3350758127032685No Hit
CTGGATGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT15991.187311582042562No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT14611.0848419145492076No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT14181.0529129601853364No Hit
CTGGATGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGACGT12730.9452455559350729No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGAT12100.8984659251228895No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT11980.8895555192539021No Hit
CTGGATGACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCT11630.8635668354693556No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA11430.8487161590210435No Hit
CTGGATGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGC10130.752186762107014No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAAT9700.7202578077431427No Hit
CTGGATGACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGACG9570.7106048680517398No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGAT9120.6771908460430373No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATAT8280.614818004960126No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGAT8270.6140754711377103No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAAT7830.5814039829514234No Hit
CTGGATGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT7050.5234863448030058No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCT6430.47744924781323794No Hit
CTGGATGACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTAGACGT5970.4432926919821198No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT4160.30889407012489417No Hit
CTGGATGACTGGGATTAGATACCCCCTATGCTTAGCCCTAAACTCTAATA3710.2754800481161917No Hit
CTGGATGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA3570.2650845746023731No Hit
CTGGATGACTGGGATTAGATACCCCGTACTAGGTGGAACTTTACAAAGAA2590.19231626000564328No Hit
CTGGATGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACA2220.16484250857626565No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT1960.14553662919345978No Hit
CTGGATGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG1870.13885382479171926No Hit
CTGGATGACTGGGATTAGATACCCCACAGATACCCGAAGCAAAGCCACGC1830.13588368950205681No Hit
CTGGATGACTGGGATTAGATACCCCGGTACCTGTCGATGAGTCTTCGGTT1740.12920088510031633No Hit
CTGGATGACTGGGATTAGATACCCCTGAAGGTGCACTGATGACAGAGGAG1730.1284583512779007No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGAC1510.11212260718475728No Hit
CTGGATACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA1490.11063753953992604No Hit
CTGGATGACTGGGATTAGATACCCCACCTACAGAGGAGCCTGTTCTAGAC1450.10766740425026361No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCACC1420.10543980278301676No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAAC1370.1017271336709387No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT550.0294.99488
TACTGGG500.0294.99486
TCGTGTC108.457191E-4294.99478295
TGGATGG157.226712E-6294.994782
CTGGGTT400.0294.994789
CTGGATT850.0294.994789
ACTGGGT400.0294.994788
TGACTGG132300.0294.883336
GACTGGG131400.0294.658057
CTGGATG134050.0294.554661
GATGACT133150.0293.887054
GGATGAC133950.0293.673433
ATGACTG133150.0293.66555
TGGATGA134250.0293.566532
ACTGGGA131650.0293.314248
CTGGGAT131800.0293.204229
ATACTGG550.0268.17715
GACTGGA1050.0252.852687
CGGGATT650.0249.610989
GACGGGA650.0249.610987
Produced by FastQC (version 0.11.5)