FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-197-6-2-6-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-197-6-2-6-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131033
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA7358856.15989865148474No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA76055.803881464974472No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA41243.1472987720650525No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA34772.6535300267871453No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGATTGAGAC28802.1979196080376697No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA27012.061312799065884No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA24831.8949424954019216No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA24151.8430471713232544No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT23971.8293101737730189No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA17051.3011989346195234No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAG15501.1829081223813849No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATA11380.868483511787107No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGATTGA10790.8234566864835575No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCGTAAACCCAGACG9970.7608770309769295No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA9830.7501926995489685No Hit
GCCACAACTGGGATTAGATACCCCAGATCCTTAATGTTTAGGAATAAGCC9710.741034701182145No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA8960.6837972113894972No Hit
GCCACAACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTA8200.6257965550662811No Hit
GCCACAACTGGGATTAGATACCCCGGGATCCATGTACCCATACGATGTTC7310.5578747338456724No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA7250.5532957346622607No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA6200.47316324895255396No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGATG5890.44950508650492627No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA5620.42889959017957313No Hit
GCCACAACTGGGATTAGATACCCCACTATACCTGGCCCTAAATCTTGATA5590.42661009058786714No Hit
GCCACAACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATA5420.4136362595682004No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAACCCTAAACCTCAACA5000.3815832652843177No Hit
GCCACAACTGGGATTAGATACCCCCGGGATCCATGTACCCATACGATGTT4880.37242526691749406No Hit
GCCACAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG4820.36784626773408224No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTAACA4120.3144246105942778No Hit
GCCACAACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGATTG3840.29305594773835597No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATA3800.29000328161608147No Hit
GCCACAACTGGGATTAGATACCCCCAAAGAGTTGAGTTAACGAGGAGCCT3710.2831347828409637No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA3070.23429212488457105No Hit
GCCACAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG2520.19231796570329612No Hit
GCCACAACTGGGATTAGATACCCCGACGTGCCTAGAGGAGCCTGTTCTAG2410.18392313386704112No Hit
GCCACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG2280.17400196896964887No Hit
GCCACAACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGATTG2240.1709493028473743No Hit
GCCACAACTGGGATTAGATACCCCACCATGCTTAGCCCTAAACCTCAACA2010.1533964726442957No Hit
GCCACAACTGGGATTAGATACCCCTCGTCGGCAGCGTCAGATGTGTAGAG1930.14729114039974664No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACA1790.13660680897178573No Hit
GCCACAACTGGGATTGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1630.12439614448268757No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAACA1490.11371181305472666No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACA1420.10836964734074622No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA1410.10760648081017758No Hit
GCCACAATGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1380.10531698121847169No Hit
GCCACAACTGGGATTAATACCCCACTATGCTTAGCCCTAAACCTCAACAG1350.10302748162676577No Hit
GCCACAACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1330.1015011485656285No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCATT108.457402E-4294.989329
ATGGGAT157.226803E-6294.989327
CTGGGTT400.0294.989328
AACTGGA650.0294.989326
CAACTGG129050.0294.989325
AAGGGAT108.457402E-4294.989326
CCACAGG157.226803E-6294.989322
AATGGGA157.226803E-6294.989326
ACGGGAT108.457402E-4294.989327
GACTGGG108.457402E-4294.989326
TGGGTTA400.0294.989329
ACAAGGG108.457402E-4294.989324
TGGGATA305.456968E-12294.989329
ACTGGTT108.457402E-4294.989327
ACTGGGT400.0294.989327
AACGGGA108.457402E-4294.989326
CACAGGG108.457402E-4294.989323
CGACTGG108.457402E-4294.989325
CAACGGG108.457402E-4294.989325
AACTGGG128200.0294.874276
Produced by FastQC (version 0.11.5)