FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-196-6-1-2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-196-6-1-2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130385
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA5291740.58519001418875No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA75655.802047781569966No Hit
CAACACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACCACTCCTG54084.147716378417763No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA40363.0954480960233157No Hit
CAACACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT29152.2356866203934502No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA29062.2287839858879472No Hit
CAACACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA26011.994861372090348No Hit
CAACACACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATA24641.8897879357288032No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA20531.5745676266441693No Hit
CAACACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA19901.5262491851056488No Hit
CAACACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA17261.3237719062775626No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA16691.280055221076044No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA15571.1941557694520075No Hit
CAACACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA13981.0722092265214558No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA13961.0706753077424551No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA12670.9717375464969129No Hit
CAACACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAC9960.7638915519423246No Hit
CAACACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTACCACTCC9710.7447175672048165No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGATG9110.6987000038347969No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA9030.6925643287187944No Hit
CAACACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACCACTCCT8420.6457798059592744No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA8380.6427119684012731No Hit
CAACACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTACC7280.5583464355562373No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT6430.49315488744870956No Hit
CAACACACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTACCAC6110.4686121869846992No Hit
CAACACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTACCACTC5310.4072554358246731No Hit
CAACACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACCACT5260.4034206388771715No Hit
CAACACACTGGGATTAGATACCCCCCCTAGAGGAGCCTGTTCTACCACTC5070.38884841047666524No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATA4910.37657706024466003No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA4740.3635387506231545No Hit
CAACACGCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA4310.3305594968746405No Hit
CAACACACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTACCACTC4240.3251907811481382No Hit
CAACACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG4130.31675422786363466No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAC4040.30985159335813167No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGATA3830.29374544617862486No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATA3700.2837749741151206No Hit
CAACACACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATA3360.2576983548721095No Hit
CAACACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT3250.24926180158760594No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTACCCCTAAACTCTAATA3180.24389308586110367No Hit
CAACACACTGGGATTAGATACCCCATTATTCTCTTTTTTAATTTTTTTTT2990.22932085746059744No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAGTA2870.22011734478659356No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA2740.21014687272308932No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCGTAAACCCAGACG2720.20861295394408863No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAGATA2600.19940944127008475No Hit
CAACACACTGGGATTAGACACCCCACTATGCTTAGCCCTAAACCTCAACA2600.19940944127008475No Hit
CAACACACTGGGATTAGATACCCCTGGCGAGAGACTGTCTCTAGAGGAGC2360.18100241592207694No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAGATA2280.1748667408060743No Hit
CAACACACTGGGATTAGATACCCCACGAGACGTCTAGAGGAGCCTGTTCT2280.1748667408060743No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACA2220.17026498446907237No Hit
CAACACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG2070.15876059362656747No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGACA2050.15722667484756683No Hit
CAACACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAGCTCTAATA2000.1533918779000652No Hit
CAACACACTGGGATTAGATACCCCCTCGTCGGCAGCGTCAGATGTGTAGA1990.15262491851056487No Hit
CAACACACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTACCACTCC1980.15185795912106453No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTTGGTA1980.15185795912106453No Hit
CAACACACTGGGATTAGATACCCCCGAGAGGAGCCTGTTCTACCACTCCT1920.1472562027840626No Hit
CAACACACTGGGATTAGATACCCCGTCTCGTCTAGAGGAGCCTGTTCTAC1890.1449553246155616No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAACA1840.14112052766805996No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA1790.13728573072055836No Hit
CAACACACTGGGATTAGATACCCCACGAGAGGAGCCTGTTCTACCACTCC1780.13651877133105803No Hit
CAACACACTGGGATTAGATACCCCACGAGACAGAGGAGCCTGTTCTACCA1770.1357518119415577No Hit
CAACACACTGGGATTAGATACCCCCCCGTCCTAGAGGAGCCTGTTCTACC1770.1357518119415577No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAACA1750.13421789316255703No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCCAAATA1710.13115005560455575No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACA1710.13115005560455575No Hit
CAACACCCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA1700.1303830962150554No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAACA1650.1265482992675538No Hit
CAACACACTGGGATTAGATACCCCTCGTCGGCAGCGTCAGATGTGTATAG1610.12348046170955249No Hit
CAACACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTACCACT1590.12194654293055184No Hit
CAACACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA1590.12194654293055184No Hit
CAACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1570.12041262415155118No Hit
CAACACACTGGGATTAGATACCCCTACTACAGGCGACCGTTCACATGGGC1530.11734478659354987No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGATG1520.11657782720404955No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACG1520.11657782720404955No Hit
CAACACACTGGGATTAGATACCCCTCCGAGCCCACGAGACTAGAGGAGCC1430.10967519269854663No Hit
CAACACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG1420.10890823330904628No Hit
CAACACACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1400.10737431453004564No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATG1360.10430647697204432No Hit
CAACACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTA1330.10200559880354336No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGGG255.293259E-10294.991585
CACACGG255.293259E-10294.991584
TGGGTTA157.226501E-6294.991559
CGCTGGG1400.0294.991556
ACGGGAT206.164919E-8294.991557
CTGGGTT157.226501E-6294.991558
CACCCTG206.164919E-8294.991554
ACTGGTT108.4571604E-4294.991557
ACTGGGT157.226501E-6294.991557
CCCTGGG206.164919E-8294.991556
CACTGGG126500.0294.641786
ACTGGGA126350.0294.641337
CTGGGAT128150.0294.416088
ACACTGG127650.0294.182745
TGGGATT128000.0293.95459
CACACTG128400.0292.8094
GCTGGGA1500.0285.15857
ACGCTGG1450.0284.819465
ACACACT135850.0277.40283
CACGCTG1500.0275.325474
Produced by FastQC (version 0.11.5)