FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-195-6-4-6-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-195-6-4-6-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122535
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA5614145.81629738442078No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA74916.113355367854083No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAACTTCGCCT36422.9722120210552085No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATA35452.8930509650303993No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT32192.627004529318154No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA29342.3944179214102093No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAA24491.9986126412861631No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA21931.7896927408495533No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA17671.442036969029257No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA14501.183335373566736No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA12100.9874729669074143No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA12070.9850246868241727No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA11510.9393234586036643No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAACTTCG10940.8928061370220752No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA10710.8740359897172237No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA8470.6912310768351899No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTTATG8410.6863345166687069No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA6670.5443342718406986No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAA5720.46680540253805036No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA5420.4423226017056351No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAGTA5110.41702370751213935No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAACA5020.40967886726241476No Hit
GCCACAACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAAC4840.3949891867629657No Hit
GCCACAACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAACTTCG4680.38193169298567753No Hit
GCCACAACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAACTTCG4400.35908107887542334No Hit
GCCACAACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAATA4100.3345982780430081No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACATTGGCA3990.32562125107112255No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAGTCTTGATA3870.31582813073815647No Hit
GCCACAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG3810.31093157057167337No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGACA3690.30113845023870733No Hit
GCCACAACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAACTTCG3680.3003223568776268No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA3560.29052923654466073No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGATG3550.2897131431835802No Hit
GCCACAACTGGGATTAGATACCCCAGAAAGAGAGGTGCGTGCGCGCGTGT3550.2897131431835802No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACA3540.2888970498224997No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCTCAACA3490.28481658301709717No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCCAAACCTCAACA3340.2725751826008896No Hit
GCCACAACTGGGATTAGATACCCCCCCGTCCTAGAGGAGCCTGTTCTAAC3280.2676786224344065No Hit
GCCACAACTGGGATTAGATACCCCTACCTGATGCCGCAGAGAGTGCCAAG3260.2660464357122455No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA3170.2587015954625209No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCATAAACTCTAATA3150.2570694087403599No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCTCTAAACTCCAATA3070.25054066185171586No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA2800.22850614110254214No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA2730.22279348757497855No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCTTAAACATTGGCA2570.20973599379769045No Hit
GCCACAACTGGGATTAGATACCCCTGACTAGAGGAGCCTGTTCTAACTTC2550.20810380707552945No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAACA2430.19831068674256336No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAA2430.19831068674256336No Hit
GCCACAACTGGGATTAGATACCCCAATACGCTTAGCCCTAAACTCCAATA2410.19667850002040235No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACA2380.1942302199371608No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGATA2360.1925980332149998No Hit
GCCACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG2330.19014975313175828No Hit
GCCACAACTGGGATTAGATACCCCCCCTAGAGGAGCCTGTTCTAACTTCG2270.18525319296527523No Hit
GCCACAACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAAC2220.18117272615987268No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACATTGGTA2190.17872444607663118No Hit
GCCACAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG2170.17709225935447015No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGATG2170.17709225935447015No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA2140.17464397927122863No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATCGATT2000.1632186722161015No Hit
GCCACAACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCT1990.16240257885502102No Hit
GCCACAACTGGGATTAGATACCCCTAGATACCCCTTACATACCCCTAGAT1840.1501611784388134No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATAGATA1790.14608071163341085No Hit
GCCACAACTGGGATTAGATACCCCATCGTAATCAGCGATAGAGGAGCCTG1750.14281633818908884No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAA1740.14200024482800833No Hit
GCCACAACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAACTTC1710.1395519647447668No Hit
GCCACAACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTAAC1710.1395519647447668No Hit
GCCACAACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTAA1690.13791977802260577No Hit
GCCACAACTGGGATTAGATACCCCACTATACTTAGCCCTGAACCTCAACA1680.13710368466152528No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAGCA1680.13710368466152528No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGCAAACTTTTATG1660.13547149793936428No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAGATA1650.13465540457828376No Hit
GCCACAACTGGGATTAGATACCCCGTGTAGTCCCTAGAGGAGCCTGTTCT1580.1289427510507202No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTTGGTA1560.1273105643285592No Hit
GCCACAACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAACTTCGCC1540.1256783776063982No Hit
GCCACAACTGGGATTAGATACCCCGCACTAGAGGAGCCTGTTCTAACTTC1490.12159791080099563No Hit
GCCACAACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATA1450.11833353735667361No Hit
GCCACAACTGGGATTAGATACCCCGACGAACCTAGAGGAGCCTGTTCTAA1440.11751744399559311No Hit
GCCACAACTGGGATTAGATACCCCCGAGCCCACCTAGAGGAGCCTGTTCT1430.11670135063451259No Hit
GCCACAACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAA1430.11670135063451259No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAACTTCGC1410.11506916391235157No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATA1380.11262088382911005No Hit
GCCACAACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAGCTCTAATA1330.10854041702370752No Hit
GCCACAACTGGGATTAGATACCCCACGAGACAGAGGAGCCTGTTCTAACT1310.1069082303015465No Hit
GCCACAACTGGGATTAGATACCCCCGATACCTAGAGGAGCCTGTTCTAAC1300.10609213694046599No Hit
GCCACAACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAACTTCG1280.10445995021830497No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGGG305.456968E-12294.991465
CACTGGG600.0294.991465
CGGGATT305.456968E-12294.991468
CAATGGG400.0294.991425
CAACTGG120300.0294.991425
CGACTGG108.456551E-4294.991425
ACTGGAT800.0294.991427
AACTGGG119500.0294.621126
GCCACAA122000.0293.903321
ACTGGGA119800.0293.26787
CTGGGAT119850.0293.022348
TGGGATT120000.0291.918589
ACAACTG123250.0288.17014
CCACAAC124750.0286.005742
CACAACT125900.0282.456053
TGGATTA900.0262.21469
CTGGGTT400.0258.11758
TGGGATA400.0258.11759
TGGGTTA351.2732926E-11252.849819
AACGGGA351.2732926E-11252.849816
Produced by FastQC (version 0.11.5)