FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-194-6-2-6-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-194-6-2-6-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120835
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA5801748.01340671163156No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA67545.589440145653164No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACAGATCTGT54774.532627136177432No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA45163.7373277609963997No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT41093.400504820623164No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA30322.5092067695617994No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA21871.8099060702610998No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA18631.541771837629826No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA18241.5094964207390242No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA17381.4383249886208467No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA17301.4317043902842719No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA15911.316671494186287No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA13231.0948814499110358No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA12411.0270203169611454No Hit
GCCACAACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACAGATCTG11520.9533661604667522No Hit
GCCACAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT9130.755575785161584No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCGTAAACCTTGATA8650.7158521951421359No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA7520.6223362436380188No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATA5830.4824761037778789No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT5340.44192493896635904No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTAACA5020.4154425456200604No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAGCCTCAACA4890.4046840733231266No Hit
GCCACAACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAATA4630.3831671287292589No Hit
GCCACAACTGGGATTAGATACCCCACCATGCTTAGCCCTAAACCTCAACA4590.37985682956097155No Hit
GCCACAACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTACA4500.37240865643232507No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGATA4480.3707535068481814No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATA4310.35668473538296025No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA4270.3533744362146729No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAC3610.2987544999379319No Hit
GCCACAACTGGGATTAGATACCCCCCCTAGAGGAGCCTGTTCTACAGATC3580.2962717755617164No Hit
GCCACAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG3560.2946166259775727No Hit
GCCACAACTGGGATTAGATACCCCTGAGCCCACGAGACCTAGAGGAGCCT3310.2739272561757769No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTCGATA3240.26813423263127406No Hit
GCCACAACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTACAGATC3200.2648239334629867No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTACAGATCT3170.2623412090867712No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAC3020.24992758720569372No Hit
GCCACAACTGGGATTAGATACCCGTCTCTAGAGGAGCCTGTTCTACAGAT2990.24744486282947822No Hit
GCCACAACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC2960.2449621384532627No Hit
GCCACAACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACAGAT2860.2366863905325444No Hit
GCCACAACTGGGATTAGATACCCCGACGACTAGAGGAGCCTGTTCTACAG2720.22510034344353871No Hit
GCCACAACTGGGATTAGATACCCCTCCTAGAGGAGCCTGTTCTACAGATC2720.22510034344353871No Hit
GCCACAACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTACAGATC2670.22096246948317952No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC2580.21351429635453303No Hit
GCCACAACTGGGATTAGATACCCCAATCTAGAGGAGCCTGTTCTACAGAT2570.2126867215624612No Hit
GCCACAACTGGGATTAGATACCCCGACGACGAGGAGCCTGTTCTACAGAT2570.2126867215624612No Hit
GCCACAACTGGGATTAGATACCCAACTATGCTTAGCCCTAAACTATAATA2500.20689369801795837No Hit
GCCACAACTGGGATTAGATACCCCCGGCTAGAGGAGCCTGTTCTACAGAT2500.20689369801795837No Hit
GCCACAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG2410.1994455248893119No Hit
GCCACAACTGGGATTAGATACCCCTCCCTAGAGGAGCCTGTTCTACAGAT2380.19696280051309636No Hit
GCCACAACTGGGATTAGATACCCCCATCTAGAGGAGCCTGTTCTACAGAT2110.17461828112715685No Hit
GCCACAACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA2070.17130798195886954No Hit
GCCACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1930.15972193486986389No Hit
GCCACAACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTACAGATCT1880.1555840609095047No Hit
GCCACAACTGGGATTAGATACCCCCCCCTAACAGGCTCCTCGTCTCGTCT1750.14482558861257086No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACA1370.11337774651384118No Hit
GCCACAACTGGGATTAGATACCCTCTAGAGGAGCCTGTTCTACAGATCTG1310.10841229776141019No Hit
GCCACAACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTACAGA1260.10427442380105101No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA1210.10013654984069185No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGGG400.0294.980565
TGGGTTA206.164191E-8294.980569
AATGGGA108.4573403E-4294.980566
CCACTGG108.4573403E-4294.980565
CAATGGG108.4573403E-4294.980565
CTGGGTT206.164191E-8294.980568
CGACTGG108.4573403E-4294.980565
CTGGGAA108.4573403E-4294.980568
TGGGATA350.0294.980539
CAACTGG118750.0294.856355
AACTGGG117600.0294.855136
ACTGGGA118150.0293.482547
CTGGGAT118050.0293.48138
TGGGATT117850.0293.103279
GCCACAA120850.0292.539671
CACAACT122750.0286.32943
CCACAAC123750.0285.08832
AACTGGA1150.0282.15536
CTGGATT1050.0280.933878
ACAACTG124950.0280.57974
Produced by FastQC (version 0.11.5)