FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-193-6-2-6-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-193-6-2-6-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119102
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA5694947.81531796275461No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA47904.0217628587261345No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA46223.8807072929085993No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA39623.3265604271968563No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA36793.088948968111367No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT33242.7908851236755052No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA31592.6523484072475694No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA31142.614565666403587No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA26102.1913989689509834No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA25852.170408557370993No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAATAGGATTC23381.963023290960689No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA18861.583516649594465No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA16771.4080368087857467No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA12981.0898221692330945No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAACA10910.916021561350775No Hit
GCCACAACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGTT8800.7388624876156572No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAA8010.6725327870228879No Hit
GCCACAACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAATAGGATT7050.5919296065557254No Hit
GCCACAACTGGGATTAGATACCCCGTGTAGTCCCTAGAGGAGCCTGTTCT6730.5650618797333378No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATA5590.46934560292858224No Hit
GCCACAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT5560.46682675353898345No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAA5400.45339289012778966No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAGTA4730.3971385870934157No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCCAAATA4190.35179929808063676No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAGCCCTAAACCTCAACA4160.3492804486910379No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATCAACA3660.3072996255310574No Hit
GCCACAACTGGGATTAGATACCCCCGTCTCGTGTCTCTAGAGGAGCCTGT3630.3047807761414586No Hit
GCCACAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG3530.2963846115094625No Hit
GCCACAACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA3390.2846299810246679No Hit
GCCACAACTGGGATTAGATACCCCTTGTTTTAGAGCTAGAGGAGCCTGTT3390.2846299810246679No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA3300.27707343285587144No Hit
GCCACAACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATA3170.2661584188342765No Hit
GCCACAACTGGGATTAGATACCCCACAATGCTTAGCCCTAAACCTCAACA3040.25524340481268154No Hit
GCCACAACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC3020.25356417188628233No Hit
GCCACAACTGGGATTAGATACCCCGCCACCTAGAGGAGCCTGTTCTAATA2840.23845107554868933No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA2710.2275360615270944No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATA2640.22165874628469717No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAATAGGA2610.2191398968950983No Hit
GCCACAACTGGGATTAGATACCCGCTAGAGGAGCCTGTTCTAATAGGATT2560.21494181457910028No Hit
GCCACAACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTA2350.19730986885190846No Hit
GCCACAACTGGGATTAGATACCCCCGTCTCGTCTCTAGAGGAGCCTGTTC2130.178838306661517No Hit
GCCACAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG2080.17464022434551896No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAACA2050.17212137495592011No Hit
GCCACAACTGGGATTAGATACCCCTTATAAGAGACAGAATCCTATTAGAA2010.1687629091031217No Hit
GCCACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG2000.1679232926399221No Hit
GCCACAACTGGGATTAGATACCCCCTCTTCGCGCTAGAGGAGCCTGTTCT1980.16624405971352288No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATA1960.16456482678712364No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACA1930.16204597739752483No Hit
GCCACAACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAATAGGAT1840.15448942922872833No Hit
GCCACAACTGGGATTAGATACCCCCTCGTGGGCTAGAGGAGCCTGTTCTA1710.14357441520713338No Hit
GCCACAACTGGGATTAGATACCCCTCGCTAGAGGAGCCTGTTCTAATAGG1520.12762170240634077No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACA1460.12258400362714311No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC1360.11418783899514702No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGTT255.293259E-10294.979468
TGGGTTA255.293259E-10294.979469
ACTGGGT255.293259E-10294.979467
ACCGGGA108.457286E-4294.979437
AACCGGG108.457286E-4294.979436
TGGTATT108.457286E-4294.979439
ACTTGGA157.2261228E-6294.979437
AACTGGG115950.0294.979436
CTGGGGT108.457286E-4294.979438
CTGGGAA108.457286E-4294.979438
CAACCGG108.457286E-4294.979435
ACACTGG550.0294.979434
CACTGGG550.0294.979435
AGGGATT157.2261228E-6294.979437
CTTGGAT157.2261228E-6294.979438
TGGGGTT108.457286E-4294.979439
CTGCGAT108.457286E-4294.979438
ACAAGGG157.2261228E-6294.979434
CCGGGAT108.457286E-4294.979438
ACTGGGG157.2261228E-6294.979437
Produced by FastQC (version 0.11.5)