FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-192-6-1-2-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-192-6-1-2-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118975
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA4624638.870350914057575No Hit
CAACACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAATAGGATTC70385.915528472368145No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA56204.7236814456818665No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA31052.6097919731035932No Hit
CAACACACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATA27012.270224837150662No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA25022.1029628073124607No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA23611.9844505148140366No Hit
CAACACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT23421.9684807732716958No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA18481.5532674931708341No Hit
CAACACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA18411.5473839041815507No Hit
CAACACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA17221.447362891363732No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA15601.3111998318974574No Hit
CAACACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAA12181.0237444841353225No Hit
CAACACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA11500.9665896196679975No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA11460.9632275688169785No Hit
CAACACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAAT11360.9548224416894304No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA11190.9405337255725992No Hit
CAACACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAATAGGATT10660.8959865517965959No Hit
CAACACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA9380.7884009245639841No Hit
CAACACACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTAATAG8840.7430132380752259No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGATG8700.7312460600966589No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA8300.6976255515864677No Hit
CAACACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAATAGGAT7920.665686068501786No Hit
CAACACACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTAATAGGA6410.5387686488758142No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA5610.47152763185543184No Hit
CAACACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT5050.4244589199411641No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT5010.421096869090145No Hit
CAACACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAATAGGA4370.36730405547383904No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTTGGTA4000.33620508510191216No Hit
CAACACACTGGGATTAGATACCCCCCCTAGAGGAGCCTGTTCTAATAGGA3890.3269594452616096No Hit
CAACACGCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA3870.3252784198361No Hit
CAACACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAATAGG3790.31855431813406176No Hit
CAACACACTGGGATTAGATACCCCGTCTCTCTAGAGGAGCCTGTTCTAAT3640.3059466274427401No Hit
CAACACACTGGGATTAGATACCCCTCTATACTAAATTACTTTATCCTATT3610.3034250893044757No Hit
CAACACACTGGGATTAGATACCCCACGAGACGTCTAGAGGAGCCTGTTCT3520.2958604748896827No Hit
CAACACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG3520.2958604748896827No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGATA3480.29249842403866355No Hit
CAACACACTGGGATTAGATACCCCACGAGACAGAGGAGCCTGTTCTAATA3280.27568816978356797No Hit
CAACACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAATAGGA3020.25383483925194367No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAA3020.25383483925194367No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAGATA2950.24795125026266024No Hit
CAACACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTA2760.23198150872031942No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAACA2700.22693843244379072No Hit
CAACACACTGGGATTAGATACCCCCCCGTCCTAGAGGAGCCTGTTCTAAT2640.22189535616726203No Hit
CAACACACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATA2510.2109686909014499No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA2430.20424458919941166No Hit
CAACACACTGGGATTAGATACCCCGCACTAGAGGAGCCTGTTCTAATAGG2430.20424458919941166No Hit
CAACACACTGGGATTAGACACCCCACTATGCTTAGCCCTAAACCTCAACA2360.19836100021012817No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATA2340.1966799747846186No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATA2210.18575330951880648No Hit
CAACACACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGC2160.18155074595503257No Hit
CAACACACTGGGATTAGATACCCCGTCTCGTCTAGAGGAGCCTGTTCTAA2010.16894305526371087No Hit
CAACACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1990.1672620298382013No Hit
CAACACACTGGGATTAGATACCCCTCCGAGCCCACGAGACTAGAGGAGCC1980.1664215171254465No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAACA1970.16558100441269175No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCGTAAACCCAGACG1850.15549485185963438No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTACCCCTAAACTCTAATA1820.15297331372137005No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACA1800.15129228829586047No Hit
CAACACACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTAATAGGAT1800.15129228829586047No Hit
CAACACACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAATA1570.13196049590250053No Hit
CAACACACTGGGATTAGATACCCCTCGTCGGCAGCGTCAGATGTGTATAG1570.13196049590250053No Hit
CAACACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA1560.13111998318974574No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATCGATT1520.12775793233872662No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTGGCCGTAAACCTTGATA1480.1243958814877075No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA1430.12019331792393359No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA1420.1193528052111788No Hit
CAACACACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATA1420.1193528052111788No Hit
CAACACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC1390.11683126707291448No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAGCCTCAACA1360.11430972893465015No Hit
CAACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1330.1117881907963858No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACA1320.11094767808363101No Hit
CAACACACTGGGATTAGATACCCCAGTCTAGAGGAGCCTGTTCTAATAGG1300.10926665265812145No Hit
CAACACACTGGGATTAGATACCCCGTCTCGTAGAGGAGCCTGTTCTAATA1290.10842613994536668No Hit
CAACACACTGGGATTAGATACCCCACCATGCTTAGCCCTAAACCTCAACA1280.10758562723261189No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGATT108.456013E-4294.99419
ACACGGG400.0294.99415
CACGGGG108.456013E-4294.99416
GTGGGAT157.224664E-6294.99418
AGTGGGA157.224664E-6294.99417
CACTGGT108.456013E-4294.99416
CACTGGG114900.0294.99416
ACGGGAT305.456968E-12294.99417
CACCTGG157.224664E-6294.99414
CGGGGAT108.456013E-4294.99418
ACGCTGG800.0294.99415
CTGGGTT157.224664E-6294.99418
CACCCTG400.0294.99414
CCCTGGG400.0294.99416
ACACTGG115850.0294.86685
ACTGGGA115300.0293.842777
CTGGGAT116600.0293.476148
TGGGATT116350.0292.96589
CACACTG117050.0292.347874
GCTGGGA850.0277.64157
Produced by FastQC (version 0.11.5)