FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-191-6-1-2-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-191-6-1-2-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117690
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA4856341.263488826578296No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA57574.891664542442008No Hit
CAACACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGACGTGGCT49634.217010791061263No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA36063.063981646698955No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA25022.12592403772623No Hit
CAACACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT24842.110629620188631No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA23181.9695811028974424No Hit
CAACACACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATA21411.8191859971110544No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA21301.8098394086158553No Hit
CAACACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA18151.5421871017078765No Hit
CAACACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA17811.513297646359079No Hit
CAACACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA14321.2167558841023027No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA13541.150480074772708No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA13511.1479310051831082No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA13291.1292378281927096No Hit
CAACACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA11891.0102812473447191No Hit
CAACACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGACGTGG8650.7349817316679411No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA8480.7205370039935424No Hit
CAACACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAG8080.6865494094655451No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA7330.6228226697255501No Hit
CAACACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAGA7150.6075282521879514No Hit
CAACACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGACGTGGC5710.4851729118871612No Hit
CAACACACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTAGACG5620.4775257031183618No Hit
CAACACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGACGT4950.4205964822839663No Hit
CAACACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT4750.4036026850199677No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTACCCCTAAACTCTAATA4700.3993542357039681No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA4600.3908573370719687No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGATG4250.3611181918599711No Hit
CAACACACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTAGACGTG3960.3364771858271731No Hit
CAACACACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATA3860.32798028719517375No Hit
CAACACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGACGTG3860.32798028719517375No Hit
CAACACGCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA3770.3203330784263744No Hit
CAACACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG3740.3177840088367746No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGATA3640.3092871102047753No Hit
CAACACACTGGGATTAGATACCCCTCTATACTAAATTACTTTATCCTATT3320.28209703458237745No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT3290.2795479649927777No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATA3170.2693516866343784No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAACA2890.24556037046478035No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAACA2870.24386099073838047No Hit
CAACACACTGGGATTAGATACCCCCCCGTCCTAGAGGAGCCTGTTCTAGA2850.2421616110119806No Hit
CAACACACTGGGATTAGACACCCCACTATGCTTAGCCCTAAACCTCAACA2600.22091936443198232No Hit
CAACACACTGGGATTAGATACCCCACCATGCTTAGCCCTAAACCTCAACA2550.21667091511598266No Hit
CAACACACTGGGATTAGATACCCCTCGTCGGCAGCGTCAGATGTGTATAG2440.20732432662078343No Hit
CAACACACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGC2420.20562494689438357No Hit
CAACACACTGGGATTAGATACCCCGCACTAGAGGAGCCTGTTCTAGACGT2350.19967711785198405No Hit
CAACACACTGGGATTAGATACCCCACGAGAGGAGCCTGTTCTAGACGTGG2300.19542866853598434No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA2280.1937292888095845No Hit
CAACACACTGGGATTAGATACCCCTGGCGAGAGACTGTCTCTAGAGGAGC2220.1886311496303849No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCGTAAACCCAGACG2080.17673549154558585No Hit
CAACACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG2070.17588580168238593No Hit
CAACACACTGGGATTAGATACCCCACGAGACAGAGGAGCCTGTTCTAGAC2040.17333673209278613No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACA2030.1724870422295862No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTTGGTA2000.16993797263998642No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAGTA1820.15464355510238764No Hit
CAACACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTA1810.1537938652391877No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAACA1780.1512447956495879No Hit
CAACACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGACGTG1770.15039510578638796No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAG1690.14359758688078852No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA1670.14189820715438867No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGACA1560.1325516186591894No Hit
CAACACCCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA1540.13085223893278952No Hit
CAACACACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTAGACGTGG1490.1266037896167899No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAGATA1490.1266037896167899No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATCGATT1460.12405472002719006No Hit
CAACACACTGGGATTAGATACCCCGTCTCTCTAGAGGAGCCTGTTCTAGA1430.12150565043759028No Hit
CAACACACTGGGATTAGATACCCCTCCGAGCCCACGAGACTAGAGGAGCC1410.11980627071119042No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAGCCCTAAACCTCAACA1310.11130937207919109No Hit
CAACACACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAATA1220.10366216331039171No Hit
CAACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1210.10281247344719179No Hit
CAACACACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1210.10281247344719179No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATA1190.10111309372079191No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGGG550.0295.000035
ACGGGAT550.0295.000037
GCTGGGA1000.0295.07
CGCTGGG1000.0295.06
ACCCTGG500.0295.05
CAGTGGG157.223887E-6295.06
CCCTGGG500.0295.06
CACTGGG113250.0294.609286
ACACTGG114200.0294.225045
ACTGGGA113300.0293.828347
CTGGGAT114900.0293.587928
TGGGATT115100.0292.821479
CACACTG114900.0292.68934
ACACACT121100.0278.06983
CGGGATT600.0270.41678
CACCCTG550.0268.181824
CTGGATT850.0260.294138
CACGCTG1150.0256.521734
ACGCTGG1150.0256.521735
ACATGGG351.2732926E-11252.857135
Produced by FastQC (version 0.11.5)