FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-190-6-1-1-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-190-6-1-1-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112519
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG4913543.668180485073634No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG56725.040926421315511No Hit
GGTACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGACGTGGCTG46554.137079071090216No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG32992.931949270789822No Hit
GGTACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT28962.5737875381046758No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC28082.4955785245158597No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG20211.7961410961704245No Hit
GGTACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG19951.7730338876100926No Hit
GGTACACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATAT19631.7445942463050683No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG18731.6646077551346883No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG16121.432646930740586No Hit
GGTACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG15581.384655036038358No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG11811.0496005119135434No Hit
GGTACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG11240.9989424008389693No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG11230.9980536620481875No Hit
GGTACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT9750.8665203210124514No Hit
GGTACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAGA9360.8318595081719532No Hit
GGTACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAGAC8190.7278770696504591No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG8180.726988330859677No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGATGC8020.712768510207165No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG7730.686995085274487No Hit
GGTACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT7120.6327820190367849No Hit
GGTACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGACGTGGC6930.6158959820119269No Hit
GGTACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGACGTGGCT5660.5030261555826127No Hit
GGTACACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTAGACGT5070.45059056692647464No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGATAC4610.4097085825505026No Hit
GGTACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTAG4270.37949146366391456No Hit
GGTACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGACGTGG3870.34394191203263447No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATTA3750.33327704654325047No Hit
GGTACACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTAGACGTGG3290.2923950621672784No Hit
GGTACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT3260.2897288457949324No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCGTAAACCCAGACGT3230.2870626294225864No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACAG3050.2710653311885104No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATAG2730.24262568988348637No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAGTAG2730.24262568988348637No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATAG2520.22396217527706433No Hit
GGTACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGACGTG2480.2204072201139363No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATAG2330.2070761382522063No Hit
GGTACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT2240.1990774891351683No Hit
GGTACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAGCTCTAATAG2160.19196757880891227No Hit
GGTACACTGGGATTAGATACCCCTCTATACTAAATTACTTTATCCTATTA2130.18930136243656628No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAGATAG2080.18485766848265625No Hit
GGTACACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATAA1990.17685901936561824No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAACAG1890.16797163145779823No Hit
GGTACACTGGGATTAGATACCCCGCACTAGAGGAGCCTGTTCTAGACGTG1880.16708289266701623No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGACAG1780.15819550475919622No Hit
GGTACACTGGGATTAGATACCCCACGAGACAGAGGAGCCTGTTCTAGACG1760.15641802717763223No Hit
GGTACACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTAGACGTGGC1710.15197433322372222No Hit
GGTACACTGGGATTAGATACCCCCCCGTCCTAGAGGAGCCTGTTCTAGAC1700.15108559443294023No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAACAG1680.1493081168513762No Hit
GGTACACTGGGATTAGATACCCCACCATGCTTAGCCCTAAACCTCAACAG1640.1457531616882482No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAGA1630.1448644228974662No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTACCCCTAAACTCTAATAG1630.1448644228974662No Hit
GGTACACTGGGATTAGATACCCCCTCGTCGGCAGCGTCAGATGTGTAGAG1620.1439756841066842No Hit
GGTACACTGGGATTAGATACCCCGTCTCGTCTAGAGGAGCCTGTTCTAGA1600.1421982065251202No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAACAG1580.1404207289435562No Hit
GGTACACTGGGATTAGATACCCCCCCTAGAGGAGCCTGTTCTAGACGTGG1470.1306446022449542No Hit
GGTACACTGGGATTAGATACCCCTATAAGAGACAGTCTCAATCTAGAGGA1460.12975586345417217No Hit
GGTACACTGGGATTAGATACCCCCTATGCTTAGCCCTAAACTCTAATAGT1430.12708964708182618No Hit
GGTACACTGGGATTAGATACCCCGTCTCTCTAGAGGAGCCTGTTCTAGAC1290.11464730401087816No Hit
GGTACACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT1270.11286982642931416No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTTGGTAA1250.11109234884775016No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACAG1240.11020361005696815No Hit
GGTACATGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT1230.10931487126618615No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCCAAACCTCAACAG1230.10931487126618615No Hit
GGTACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGACGTGG1220.10842613247540415No Hit
GGTACACTGGGATTAGATACCCCAATACGCTTAGCCCTAAACTCCAATAG1200.10664865489384014No Hit
GGTACACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAATAG1150.10220496093993016No Hit
GGTACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT1150.10220496093993016No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACCT108.455165E-4294.996921
TTGGATT206.160553E-8294.996928
GGGAGAT108.455165E-4294.996929
CTTGGAT206.160553E-8294.996927
CTGGGAG108.455165E-4294.996927
TGGGAGA108.455165E-4294.996928
ATGGGAT550.0294.99696
TGGGTTA255.275069E-10294.99698
CACTGGG110100.0294.99695
TACATGG550.0294.99693
ACATGGG550.0294.99694
GGGTTAG255.275069E-10294.99699
CTGGGTT255.275069E-10294.99697
TGGGATA500.0294.99698
CTGGATT950.0294.99697
ACTGGGA109850.0294.99696
ACACTGG111200.0294.864264
GGTACAC111900.0294.733281
CTGGGAT109850.0294.728337
GTACACT112050.0293.680542
Produced by FastQC (version 0.11.5)