FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-19-4-4-1-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-19-4-4-1-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12076
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG209117.315336204041074No Hit
GGTACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTATT143911.916197416363033No Hit
GGTACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTATTCCTCTGTC10498.686651209009606No Hit
GGTACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTATTCCTCT7496.202384895660814No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG3743.0970520039748264No Hit
GGTACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTATTCCTCTGT2772.2938058959920506No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTATT2662.2027161311692613No Hit
GGTACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTATTCCTCTG2542.1033454786353096No Hit
GGTACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTATTCCTC2301.9046041735674062No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAT2271.8797615104339187No Hit
GGTACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTATTCCTC2001.6561775422325273No Hit
GGTACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTATTCCTCT1811.4988406757204373No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC1701.4077509108976483No Hit
GGTACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT1471.2172904935409077No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTATT1421.1758860549850945No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG1421.1758860549850945No Hit
GGTACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT1301.0765154024511427No Hit
GGTACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTATTCCTC1211.0019874130506792No Hit
GGTACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTATTC930.7701225571381252No Hit
GGTACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTATTCCTC890.7369990062934747No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAT860.7121563431599868No Hit
GGTACACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTATTCCTCT730.6045048029148725No Hit
GGTACACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTATTCCTCT670.5548194766478967No Hit
GGTACACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTATT660.546538588936734No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG600.4968532626697582No Hit
GGTACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG570.4720105995362702No Hit
GGTACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTATTCC560.4637297118251077No Hit
GGTACACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTATTCCTCT560.4637297118251077No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG540.44716793640278235No Hit
GGTACACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTATT540.44716793640278235No Hit
GGTACACTGGGATTAGATACCCCACTGACACTAGAGGAGCCTGTTCTATT530.4388870486916197No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG520.4306061609804571No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTATT510.4223252732692945No Hit
GGTACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAT490.4057634978469692No Hit
GGTACACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTATTCCT470.38920172242464396No Hit
GGTACACTGGGATTAGATACCCCTTCCGAAGTGCAGCTGTTGGAGACTAG440.36435905929115603No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAT440.36435905929115603No Hit
GGTACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTATTCCTCT420.34779728386883074No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCTAGAGGAGCCTGTTCTATTC420.34779728386883074No Hit
GGTACACTGGGATTAGATACCCCGGTAGCTAGAGGAGCCTGTTCTATTCC400.33123550844650546No Hit
GGTACACTGGGATTAGATACCCCGACGACTAGAGGAGCCTGTTCTATTCC400.33123550844650546No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG360.29811195760185494No Hit
GGTACACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTCT360.29811195760185494No Hit
GGTACACTGGGATTAGATACCCCGGGTAGTCTAGAGGAGCCTGTTCTATT340.28155018217952965No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT330.273269294468367No Hit
GGTACACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTATTC320.26498840675720436No Hit
GGTACACTGGGATTAGATACCCCACTAGACCTAGAGGAGCCTGTTCTATT320.26498840675720436No Hit
GGTACACTGGGATTAGATACCCCAAGCCCACGAGACCTAGAGGAGCCTGT310.2567075190460417No Hit
GGTACACTGGGATTAGATACCCCGATCTAGAGGAGCCTGTTCTATTCCTC300.2484266313348791No Hit
GGTACACTGGGATTAGATACCCCACGAGATCTAGAGGAGCCTGTTCTATT300.2484266313348791No Hit
GGTACACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTATT290.24014574362371646No Hit
GGTACACTGGGATTAGATACCCCAATACGACTCACTAGAGGAGCCTGTTC280.23186485591255385No Hit
GGTACACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTATTCCTCTG270.22358396820139118No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG260.21530308049022856No Hit
GGTACACTGGGATTAGATACCCCTAAGAGGAGCCTGTTCTATTCCTCTGT260.21530308049022856No Hit
GGTACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG260.21530308049022856No Hit
GGTACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTATTCCTCTG250.20702219277906592No Hit
GGTACACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCTG250.20702219277906592No Hit
GGTACACTGGGATTAGATACCCCCCCTCGTCTACGGGAGTAGAGAGCCTA250.20702219277906592No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCTCG240.1987413050679033No Hit
GGTACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTATTC230.19046041735674063No Hit
GGTACACTGGGATTAGATACCCCGAACCTAGAGGAGCCTGTTCTATTCCT220.18217952964557801No Hit
GGTACACTGGGATTAGATACCCCCGCCTAGAGGAGCCTGTTCTATTCCTC220.18217952964557801No Hit
GGTACACTGGGATTAGATACCCCGTCAGACTAGAGGAGCCTGTTCTATTC220.18217952964557801No Hit
GGTACACTGGGATTAGATACCCATAGAGGAGCCTGTTCTATTCCTCTGTC210.17389864193441537No Hit
GGTACACTGGGATTAGATACCCCAATGTCAGACTAGAGGAGCCTGTTCTA210.17389864193441537No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG210.17389864193441537No Hit
GGTACACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTATTCCT210.17389864193441537No Hit
GGTACACTGGGATTAGATACCCCTGAGTGACTAGAGGAGCCTGTTCTATT210.17389864193441537No Hit
GGTACACTGGGATTAGATACCCCGAGATGCCTAAGCTAGAGGAGCCTGTT210.17389864193441537No Hit
GGTACACTGGGATTAGATACCCCTGACGCTGGACTAGAGGAGCCTGTTCT200.16561775422325273No Hit
GGTACACTGGGATTAGATACCCCCAACTAGAGGAGCCTGTTCTATTCCTC190.15733686651209008No Hit
GGTACACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTATTCCTCT190.15733686651209008No Hit
GGTACACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTATTCCT180.14905597880092747No Hit
GGTACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG180.14905597880092747No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG170.14077509108976483No Hit
GGTACACTGGGATTAGATACCCCAGCCTAGAGGAGCCTGTTCTATTCCTC170.14077509108976483No Hit
GGTACACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCTA160.13249420337860218No Hit
GGTACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT150.12421331566743955No Hit
GGTACACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATATC150.12421331566743955No Hit
GGTACACTGGGATTAGATACCCCTGACTAGAGGAGCCTGTTCTATTCCTC140.11593242795627692No Hit
GGTACACTGGGATTAGATACCCCCCGTCTAGAGGAGCCTGTTCTATTCCT140.11593242795627692No Hit
GGTACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT140.11593242795627692No Hit
GGTACACTGGGATTAGATACCCCATGTCAGACTAGAGGAGCCTGTTCTAT140.11593242795627692No Hit
GGTACACTGGGATTAGATACCCCATACGTGCAGGGAGGGTACCTTGAGGT140.11593242795627692No Hit
GGTACACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGCC140.11593242795627692No Hit
GGTACACTGGGATTAGATACCCCCGACAACTAGAGGAGCCTGTTCTATTC140.11593242795627692No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTCAACAG140.11593242795627692No Hit
GGTACACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTATTC130.10765154024511428No Hit
GGTACACTGGGATTAGATACCCCCCACCTAGAGGAGCCTGTTCTATTCCT130.10765154024511428No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGAT11850.0295.07
CTGGGAG108.3615584E-4295.07
ACACTGG11950.0295.04
TACACGG108.3615584E-4295.03
ACTGGGA11950.0295.06
GGTACAC12050.0295.01
CACTGGG12000.0293.77085
TGGGATT11850.0293.755288
GGGATTA11850.0292.510539
GTACACT12150.0290.1442
TACACTG12150.0290.1443
CTTCATA2150.059.000004115-119
GGACCTG2150.059.000004105-109
CCTTGAT501.8189894E-1259.00000440-44
CTGGCGG2150.059.000004110-114
ACCTTGA501.8189894E-1259.00000440-44
AAGGACC2150.059.000004100-104
GACCTGG2150.059.000004105-109
GCTTCAT2150.059.000004115-119
GTAAACC501.8189894E-1259.00000435-39
Produced by FastQC (version 0.11.5)