FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-189-6-1-1-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-189-6-1-1-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95416
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG3960141.503521421983734No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG49335.169992454095749No Hit
GGTACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAATAGGATTCT43704.579944663368827No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC26912.820281713758699No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG26622.7898884883038484No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG20312.1285738240965872No Hit
GGTACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT20032.099228640898801No Hit
GGTACACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATAT16561.7355579776976608No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG15541.6286576674771527No Hit
GGTACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG15371.610840949107068No Hit
GGTACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG14831.5542466672256225No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG11941.251362454934183No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG11301.1842877504820994No Hit
GGTACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT11081.1612308208266957No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG10781.1297895531147815No Hit
GGTACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG10541.1046365389452502No Hit
GGTACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAAT8350.875115284648277No Hit
GGTACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAATAGGATT7020.735725664458791No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG6850.7179089460887064No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGATGC6350.6655068332355161No Hit
GGTACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAATA6160.6455940303513038No Hit
GGTACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAATAGGATTC5330.558606523015008No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG4940.5177328749895196No Hit
GGTACACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTAATAGG4910.5145887482183282No Hit
GGTACACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTAATAGGAT3840.4024482267125011No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTTGGTAA3750.39301584639892684No Hit
GGTACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAATAGGA3540.3710069590005869No Hit
GGTACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTAG3450.36157457868701265No Hit
GGTACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAATAGGAT3330.348998071602247No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAACAG3130.3280372264609709No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATAG3130.3280372264609709No Hit
GGTACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT3050.31965288840446043No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTACCCCTAAACTCTAATAG2980.3123165926050138No Hit
GGTACACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATAA2960.31022050809088625No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATCGATTG2530.2651546910371426No Hit
GGTACACTGGGATTAGATACCCCACGAGACAGAGGAGCCTGTTCTAATAG2500.2620105642659512No Hit
GGTACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT2490.2609625220088874No Hit
GGTACACTGGGATTAGATACCCCCCCGTCCTAGAGGAGCCTGTTCTAATA2350.2462899304099941No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATTA2320.24314580363880273No Hit
GGTACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAATAGGAT2120.22218495849752662No Hit
GGTACACTGGGATTAGATACCCCTCTATACTAAATTACTTTATCCTATTA2040.2138006204410162No Hit
GGTACACTGGGATTAGATACCCCTGACGCTAAGAGGAGCCTGTTCTAATA1940.20332019787037814No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACAG1920.20122411335625054No Hit
GGTACACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATAA1870.1959839020709315No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAGATAG1860.1949358598138677No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGACAG1860.1949358598138677No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATAG1820.19074369078561246No Hit
GGTACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT1810.18969564852854867No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGATAC1800.18864760627148486No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAAT1680.1760710991867192No Hit
GGTACACTGGGATTAGATACCCCCCCTAGAGGAGCCTGTTCTAATAGGAT1630.17083088790140016No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAGTAG1560.16349459210195355No Hit
GGTACACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAATAG1550.16244654984488974No Hit
GGTACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAGCTCTAATAG1480.15511025404544312No Hit
GGTACACTGGGATTAGATACCCCACGAGAGGAGCCTGTTCTAATAGGATT1480.15511025404544312No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAACAG1450.1519661272742517No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACAG1330.13938962018948603No Hit
GGTACACTGGGATTAGATACCCCTCGTCGGCAGCGTCAGATGTGTATAGA1320.13834157793242224No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAACAG1290.13519745116123083No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACGG1240.1299572398759118No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG1230.128909197618848No Hit
GGTACACTGGGATTAGATACCCCGCACTAGAGGAGCCTGTTCTAATAGGA1220.1278611553617842No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATAG1210.12681311310472038No Hit
GGTACACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT1180.12366898633352898No Hit
GGTACACTGGGATTAGATACCCCGTCTCGTCTAGAGGAGCCTGTTCTAAT1170.12262094407646516No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCGTAAACCCAGACGT1140.11947681730527374No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTAACAG1100.11528464827701852No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGGTAG1080.11318856376289091No Hit
GGTACACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTAATAGGATT1070.11214052150582711No Hit
GGTACACTGGGATTAGATACCCCAATACGCTTAGCCCTAAACTCCAATAG1050.11004443699169951No Hit
GGTACACTGGGATTAGATACCCCGTCTCTCTAGAGGAGCCTGTTCTAATA1010.10585226796344428No Hit
GGTACACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAATAGGAT970.10166009893518907No Hit
GGTACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT960.10061205667812527No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGA206.157279E-8294.982735
GGTACTG108.4543705E-4294.982731
GTACCTG255.275069E-10294.982732
ACTTGGA108.4543705E-4294.982736
GCTGGGA108.4543705E-4294.982736
TGGAATT108.4543705E-4294.982738
CGCTGGG108.4543705E-4294.982735
CTTGGAT108.4543705E-4294.982737
TACTGGG108.4543705E-4294.982733
CGGGATT255.275069E-10294.982737
CTGGGTT108.4543705E-4294.982737
GGGATTG206.157279E-8294.982739
CACTTGG108.4543705E-4294.982735
ACTGGAA108.4543705E-4294.982736
CACTGGT157.2213024E-6294.98275
ACTGGGT157.2213024E-6294.98276
GGTACAC94600.0294.826781
CACTGGG92950.0294.824045
ACACTGG93900.0294.668554
ACTGGGA92850.0294.506166
Produced by FastQC (version 0.11.5)