FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-188-6-4-6-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-188-6-4-6-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93042
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA4206945.21506416457084No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA54945.904860170675609No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA29453.165237204703252No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA28593.0728058296253304No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACCACTCCTG26492.847101309086219No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA20852.2409234539240344No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAC18451.9829754304507643No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA18431.9808258635884868No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA17311.8604501193009608No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT13211.4197889125341245No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATA10761.1564669719051612No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACCACTC9911.065110380258378No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA8580.9221641839169407No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA8200.8813224135336729No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTTATG7760.8340319425635734No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACA6520.7007587971023839No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA5550.5965048042819372No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGATG5490.5900561036951054No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA5410.5814578362459963No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT5170.5556630338986694No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA4490.48257776058124285No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATCGATT4320.4643064422518862No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGACA3980.427763805593173No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTATAATA3960.42561423873089577No Hit
GCCACAACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTA3840.4127168375572322No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA3770.4051933535392618No Hit
GCCACAACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACCACTC3710.39874465295243006No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTTGGTA3610.38799681864104385No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATG3530.37939855119193483No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAGATA3490.37509941746738035No Hit
GCCACAACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTACC3330.3579028825691623No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCATAAACTTTGATA3280.3525289654134692No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA3080.3310332967906967No Hit
GCCACAACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACCACTCCT2890.3106124115990628No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAC2790.29986457728767657No Hit
GCCACAACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACCACT2740.2944906601319834No Hit
GCCACAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG2730.2934158767008448No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACA2620.2815932589583199No Hit
GCCACAACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTAC2440.26224715719782465No Hit
GCCACAACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACCACT2290.24612540573074526No Hit
GCCACAACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTACCAC2220.23860192171277486No Hit
GCCACAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT2160.2321532211259431No Hit
GCCACAACTGGGATTAGATACCCCTAGTTGACTAGAGGAGCCTGTTCTAC1990.2138819027965865No Hit
GCCACAACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTACCACTCC1990.2138819027965865No Hit
GCCACAACTGGGATTAGATACCCCAAGCCCACGAGACCTAGAGGAGCCTG1930.20743320220975472No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAC1920.2063584187786161No Hit
GCCACAACTGGGATTAGATACCCCGTCTCGTCGTCGTCGTCGTCTAGAGG1870.200984501622923No Hit
GCCACAACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC1840.1977601513295071No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATA1840.1977601513295071No Hit
GCCACAACTGGGATTAGATACCCCACGAGACGTCTAGAGGAGCCTGTTCT1830.1966853678983685No Hit
GCCACAACTGGGATTAGATACCCCACTATGTATGGTCGTTAACATTGATG1780.19131145074267533No Hit
GCCACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1770.1902366673115367No Hit
GCCACAACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTACCACTC1740.18701231701812085No Hit
GCCACAACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTACCACTCC1730.18593753358698223No Hit
GCCACAACTGGGATTAGATACCCCATACGTAGGTAACGCACTCCAAGTGG1700.18271318329356634No Hit
GCCACAACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTACCACTC1700.18271318329356634No Hit
GCCACAACTGGGATTAGATACCCCTGACCCTAGAGGAGCCTGTTCTACCA1650.1773392661378732No Hit
GCCACAACTGGGATTAGATACCCCATGTGCCTCTAGAGGAGCCTGTTCTA1650.1773392661378732No Hit
GCCACAACTGGGATTAGATACCCCACTATTCTTAGCCCTAAACCTCAACA1640.1762644827067346No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG1630.17518969927559597No Hit
GCCACAACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATA1550.16659143182648697No Hit
GCCACAACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTACCACT1530.16444186496420973No Hit
GCCACAACTGGGATTAGATACCCCATACGTGCAGGGAGGGTACCTTGAGG1520.16336708153307108No Hit
GCCACAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1460.15691838094623933No Hit
GCCACAACTGGGATTAGATACCCCATTATTCTCTTTTTTAATTTTTTTTT1450.15584359751510068No Hit
GCCACAACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTACCA1450.15584359751510068No Hit
GCCACAACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAATA1430.15369403065282344No Hit
GCCACAACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAC1330.1429461963414372No Hit
GCCACAACTGGGATTAGATACCCCTCCGAGCCCACGAGACTAGAGGAGCC1240.13327314546118957No Hit
GCCACAACTGGGATTAGATACCCCCCACACACACACACACACACACACAC1240.13327314546118957No Hit
GCCACAACTGGGATTAGATACCCCATCTCCGAGCCCACGAACCTAGAGGA1230.13219836203005095No Hit
GCCACAACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTACCAC1210.1300487951677737No Hit
GCCACAACTGGGATTAGATACCCCGTCTCTCTAGAGGAGCCTGTTCTACC1110.11930096085638743No Hit
GCCACAACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1060.11392704370069431No Hit
GCCACAACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTACCACT1060.11392704370069431No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTACCACTCC1050.1128522602695557No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGTCCCTAGAGGAGCCTGTTCT1020.1096279099761398No Hit
GCCACAACTGGGATTAGATACCCCAGAAGGACATATGCCAATTGAAGTCT1020.1096279099761398No Hit
GCCACAACTGGGATTAGATACCCCTCGGAGATGTGTATAAGAGAGGAGCC940.10102964252703081No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGTTA157.220393E-6294.986029
ACGGGAT157.220393E-6294.986027
CGGATTA108.453745E-4294.986028
ACGGATT108.453745E-4294.986027
CGGGATT157.220393E-6294.986028
CAATGGG206.156006E-8294.986025
CTGGGTT157.220393E-6294.986028
CTGGATT650.0294.986028
ACTGGGT157.220393E-6294.986027
ACTGGAT650.0294.986027
ACTGGAA108.453745E-4294.986027
AACTGGG90550.0294.823126
CAACTGG91450.0294.663485
ACTGGGA90650.0293.847087
CTGGGAT90500.0293.845188
TGGGATT90450.0293.518439
GCCACAA93300.0292.614751
CCACAAC96050.0283.008452
CACAACT96350.0281.208773
ACAACTG96300.0280.435824
Produced by FastQC (version 0.11.5)