FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-187-6-2-6-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-187-6-2-6-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90877
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA4644551.10754096195957No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGGAATCGCT48065.288466828790563No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA44294.873620387996963No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA43364.7712842633449615No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA21392.3537308669960497No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA17981.9784984099387084No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA15761.734212176898445No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA14651.6120690603783134No Hit
GCCACAACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGGAATCGC13731.5108333241634297No Hit
GCCACAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT12311.3545781660926306No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA11231.235736214883854No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGGAATC9291.0222608580829031No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA8650.9518359981073319No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA8440.9287278409278475No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATA7530.8285924931500819No Hit
GCCACAACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC6920.7614687984858655No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA5990.6591326738338634No Hit
GCCACAACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTAGG5800.6382252935286157No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGACCTAAACCTCAACA4400.4841709123320532No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA4380.48197013545781664No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA4300.4731670279608702No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA3720.40934449860800864No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAG3600.396139837362589No Hit
GCCACAACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATA3490.38403556455428767No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAGACCTCAACA3460.38073439924293273No Hit
GCCACAACTGGGATTAGATACCCCATTCCTAGAGGAGCCTGTTCTAGGAA3440.37853362236869614No Hit
GCCACAACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGGAAT3340.36752973799751315No Hit
GCCACAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG2920.3213134236385444No Hit
GCCACAACTGGGATTAGATACCCCGACGATAGAGGAGCCTGTTCTAGGAA2780.30590798551888815No Hit
GCCACAACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA2540.2794986630280489No Hit
GCCACAACTGGGATTAGATACCCCTCGTCGGCAGCGTCAGATGTGTATAA2430.2673943902197476No Hit
GCCACAACTGGGATTAGATACCCCCGCAACCTAGAGGAGCCTGTTCTAGG2380.26189244803415607No Hit
GCCACAACTGGGATTAGATACCCAACTATGCTTAGCCCTAAACTATAATA2240.24648700991449982No Hit
GCCACAACTGGGATTAGATACCCCGAAACCTAGAGGAGCCTGTTCTAGGA2120.23328234866908018No Hit
GCCACAACTGGGATTAGATACCCCCTCTCCCTAGAGGAGCCTGTTCTAGG1990.21897729898654228No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATA1910.21017419148959582No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC1910.21017419148959582No Hit
GCCACAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1870.20577263774112262No Hit
GCCACAACTGGGATTAGATACCCCGTCTTCCAGAGGAGCCTGTTCTAGGA1800.1980699186812945No Hit
GCCACAACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAGG1760.1936683649328213No Hit
GCCACAACTGGGATTAGATACCCCCTATGCTTAGCCCTAAACTCTAATAG1670.18376486899875658No Hit
GCCACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1600.17606214993892844No Hit
GCCACAACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAATA1450.15955632338215392No Hit
GCCACAACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTAGGAAT1390.15295399275944407No Hit
GCCACAACTGGGATTAGATACCTCACTATGCTTAGCCCTAAACCTCAACA1070.1177415627716584No Hit
GCCACAACTGGGATTAGATACCCCTTCCTAGAGGAGCCTGTTCTAGGAAT1060.11664117433454009No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTATAATA1060.11664117433454009No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTGTCTCTTATACACATCTC1040.11444039746030348No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACA960.10563728996335706No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA950.10453690152623876No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAG940.10343651308912045No Hit
GCCACAACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGGAATC920.10123573621488384No Hit
GCCACAACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG920.10123573621488384No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAT108.452224E-4295.07
CAATGGG108.452224E-4295.05
CTGGGTT108.452224E-4295.08
AACTGGG89550.0295.06
CACTGGG206.153823E-8295.05
AATGGGA108.452224E-4295.06
ACGGGAT206.153823E-8295.07
TGGGTTA108.452224E-4295.09
TGGGATA400.0295.09
ACTGGGT108.452224E-4295.07
AACGGGA206.153823E-8295.06
CAACTGG89950.0294.50815
ACTGGGA89650.0294.34197
CTGGGAT89600.0294.341528
TGGGATT89350.0293.844429
GCCACAA90950.0293.702581
CCACAAC93150.0286.132572
CACAACT93850.0283.055423
ACAACTG93850.0282.74114
CGGGATT251.8727042E-7236.08
Produced by FastQC (version 0.11.5)