FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-186-6-7-5-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-186-6-7-5-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87954
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG4421050.264911203583694No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG71788.161084203106169No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG34823.9588876003365394No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC29313.3324237669690975No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG23202.637742456283967No Hit
AAGCGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG19852.2568615412602044No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG15381.7486413352434227No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT12601.4325670236714647No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG12491.4200604861632218No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG10731.2199558860313346No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG9831.1176296700548014No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG9491.0789730995747777No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG8971.0198512858994475No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATAG7660.870909793755827No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG7140.8117879800804966No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG6940.7890488209746003No Hit
AAGCGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT5520.6276007913227368No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAGTAG4570.5195897855697297No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG4460.5070832480614867No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATAG4110.46728971962616817No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATTA3910.4445505605202719No Hit
AAGCGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGACGTGGCTG3510.39907224230847943No Hit
AAGCGACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAGCTCTAATAG2460.2796916570025241No Hit
AAGCGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT2370.2694590354048707No Hit
AAGCGACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTAG2310.26263728767310185No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAGCCTCAACAG2280.25922641380721745No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATAG2190.2489937922095641No Hit
AAGCGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT1890.2148850535507197No Hit
AAGCGACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACCTCAACAG1890.2148850535507197No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTGAACCTTGATAG1760.2001046001318871No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGATAC1700.19328285240011825No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCGTAAACTTTGATAG1610.18305023080246494No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCTCG1560.17736544102599086No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTACCCCTAAACTCTAATAG1550.17622848307069605No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCACAACAG1500.17054369329422198No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAGATCTTGATAC1500.17054369329422198No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCTATAAACTCAAATAA1460.16599586147304274No Hit
AAGCGACTGGGATTAGATACCCCACTACACCTAGCCCTAAAGTCGAATAG1450.16485890351774793No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTCCTAAACCTCAACAG1370.1557632398753894No Hit
AAGCGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT1350.15348932396479978No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTAGATAT1340.15235236600950497No Hit
AAGCGACTGGGATTAGATACCCCACTACGCTCAGCCCTAAACTTCAACAG1300.14780453418832573No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAGTCTTGATAC1270.14439366032244128No Hit
AAGCGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAGA1270.14439366032244128No Hit
AAGCGACTGGGATTAGATACCCCAGTAGTCTACGCCCTAAACGATGATCG1260.14325670236714647No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCCAGCCGTAAACCTTGATAG1180.13416103872478796No Hit
AAGCGACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATAA1150.1307501648589035No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACAG1140.12961320690360872No Hit
AAGCGACTGGGATTAGATACCCCACAATGCTTAGCCCTAAACTTCAACAG1100.12506537508242946No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAGATAA1060.12051754326125019No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAATCTCAACAG1060.12051754326125019No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTCGATAC980.1114218796188917No Hit
AAGCGACTGGGATTAGATACCCCTCCGAGCCCACGAGACTCTCCGATATC970.11028492166359688No Hit
AAGCGACTGGGATTAGATACCCCATTATGCACAGCCGTAAACTTTGATAA920.10460013188712282No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACTG910.10346317393182801No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATGC890.10118925802123838No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACAG890.10118925802123838No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATAT890.10118925802123838No Hit
AAGCGACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT880.10005230006594355No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGGGA255.275069E-10294.95855
CGCTGGG305.456968E-12294.95854
GCGGGGA108.4553275E-4294.95853
ACTGTGA206.157097E-8294.95856
ATGGGAT255.275069E-10294.95856
GGGTTAG157.2217517E-6294.95859
GCGACTG86700.0294.95853
CTGGGTT157.2217517E-6294.95857
GCGACGG157.2217517E-6294.95853
GCGATGG350.0294.95853
CGGGGAT108.4553275E-4294.95854
GACGGGA108.4553275E-4294.95855
AGCGGGG108.4553275E-4294.95852
GCGCTGG305.456968E-12294.95853
CTGTGAT206.157097E-8294.95857
ACGGGAT108.4553275E-4294.95856
GACTGTG206.157097E-8294.95855
GTGATTA206.157097E-8294.95859
AAGCGGG108.4553275E-4294.95851
TGGGTTA157.2217517E-6294.95858
Produced by FastQC (version 0.11.5)