FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-185-6-1-2-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-185-6-1-2-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88607
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA3733442.134368616475No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA43794.9420474680329995No Hit
CAACACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACCTAGCTGT33413.770582459625086No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA25912.924148204995091No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA24362.749218459038225No Hit
CAACACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA18072.039342264155202No Hit
CAACACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT17511.9761418398094959No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA17011.9197128895008293No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA15291.7255973004390173No Hit
CAACACACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATA14601.6477253490130577No Hit
CAACACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA12331.3915379146117124No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA12251.3825092825623257No Hit
CAACACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA12091.3644520184635527No Hit
CAACACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA9641.0879501619510874No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA9261.0450641597165011No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA8790.9920209464263545No Hit
CAACACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAC7040.7945196203460223No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGATG5810.655704402586703No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA5590.6308756644508899No Hit
CAACACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT4960.5597751870619703No Hit
CAACACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTACCTAGCT4700.5304321329014637No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA4600.5191463428397305No Hit
CAACACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACCTAGCTG4410.4977033417224373No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGACA4260.48077465662983737No Hit
CAACACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTACC4230.4773889196113174No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT4050.45707449750019746No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGATA3750.42321712731499767No Hit
CAACACGCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA3230.36453101899398466No Hit
CAACACACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTACCTA2960.3340593858273048No Hit
CAACACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG2760.31148780570383827No Hit
CAACACACTGGGATTAGATACCCCACGAGACAGAGGAGCCTGTTCTACCT2750.31035922669766497No Hit
CAACACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACCTAG2730.3081020686853183No Hit
CAACACACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTACCTAGC2400.2708589614815985No Hit
CAACACACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATA2340.26408748744455857No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAGATA2170.24490164433961198No Hit
CAACACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTACCTAGC2140.24151590732109202No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATA2010.22684438024083878No Hit
CAACACACTGGGATTAGACACCCCACTATGCTTAGCCCTAAACCTCAACA1950.22007290620379877No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTTGGTA1930.21781574819145214No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTACCCCTAAACTCTAATA1890.21330143216675884No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACA1810.20427280011737223No Hit
CAACACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACCTAGC1760.19862990508650558No Hit
CAACACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTA1660.1873441150247723No Hit
CAACACACTGGGATTAGATACCCCCCCTAGAGGAGCCTGTTCTACCTAGC1650.186215536018599No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCGTAAACCCAGACG1640.18508695701242567No Hit
CAACACACTGGGATTAGATACCCCCCCGTCCTAGAGGAGCCTGTTCTACC1630.18395837800625234No Hit
CAACACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAC1630.18395837800625234No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA1600.18057264098773235No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAGTA1480.1670296929136524No Hit
CAACACACTGGGATTAGATACCCCACGAGACGTCTAGAGGAGCCTGTTCT1460.16477253490130578No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATA1390.15687248185809247No Hit
CAACACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1370.1546153238457458No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA1260.14220095477783923No Hit
CAACACACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATA1220.1376866387531459No Hit
CAACACACTGGGATTAGATACCCCACCATGCTTAGCCCTAAACCTCAACA1180.13317232272845259No Hit
CAACACACTGGGATTAGATACCCCGCACTAGAGGAGCCTGTTCTACCTAG1160.13091516471610595No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACG1140.1286580067037593No Hit
CAACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1130.12752942769758596No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAACA1090.12301511167289265No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACA1070.12075795366054601No Hit
CAACACACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTACCTAGCT1070.12075795366054601No Hit
CAACACCCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA1070.12075795366054601No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGATG1050.11850079564819935No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA1050.11850079564819935No Hit
CAACACACTGGGATTAGATACCCCTGACGCTAAGAGGAGCCTGTTCTACC1050.11850079564819935No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAACA1000.11285790061733272No Hit
CAACACACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGC960.1083435845926394No Hit
CAACACACTGGGATTAGATACCCCTCCGAGCCCACGAGACTAGAGGAGCC940.10608642658029276No Hit
CAACACACTGGGATTAGATACCCCCGAGAGGAGCCTGTTCTACCTAGCTG930.10495784757411943No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAGACCTCAACA910.10270068956177278No Hit
CAACACACTGGGATTAGATACCCCGTCTCTCTAGAGGAGCCTGTTCTACC890.10044353154942613No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTGG157.2178773E-6295.05
GCTGGGA550.0295.07
CACGCTG550.0295.04
AGTGGGA157.2178773E-6295.07
ACCCTGG255.275069E-10295.05
CAGTGGG157.2178773E-6295.06
AACTGGG303.6379788E-12295.05
CTGGGTT206.1529136E-8295.08
CACCCTG255.275069E-10295.04
CTGGATT600.0295.08
ACTGGGT206.1529136E-8295.07
ACTGGAT650.0295.07
CCCTGGG255.275069E-10295.06
ACACTGG86500.0294.82955
CACTGGG85750.0294.8286
ACTGGGA85800.0293.96857
CTGGGAT86650.0293.808448
CACACTG87100.0292.798524
TGGGATT86400.0292.780679
ACACACT90950.0280.890623
Produced by FastQC (version 0.11.5)