FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-184-6-2-6-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-184-6-2-6-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87214
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA4838555.47847822597289No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA34934.0050909257687985No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA32893.7711835255807555No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA24082.761024606141216No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT20852.3906712225101474No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA19662.2542252390671225No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA19222.2037746233402893No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA17321.9859196917926023No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTATAAGCA12971.4871465590386865No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA12301.4103240305455547No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA9201.0548765106519595No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTATAAGCACTG6740.7728117045428486No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA6700.7682252849313184No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA6520.7475863966794322No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA6310.7235076937188983No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGGTA5990.6868163368266563No Hit
GCCACAACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTATAAGC5970.6845231270208911No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA4800.550370353383631No Hit
GCCACAACTGGGATTAGATACCCCACTATACTTAGCCCTAAACCTCAACA4780.548077143577866No Hit
GCCACAACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTT4240.4861604788222075No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTCCTAAACCTCAACA3710.4253904189694315No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAT3530.4047515307175453No Hit
GCCACAACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTATAAGCA3520.4036049258146628No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGACA3400.389845666980072No Hit
GCCACAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG3230.3703533836310684No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAT3150.3611805444080079No Hit
GCCACAACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATA3060.35086110028206485No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT3010.345128075767652No Hit
GCCACAACTGGGATTAGATACCCCACTACGCCTGGCCCTAAATCTTGATA2980.3416882610590043No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCTGTAAACTTTGATA2980.3416882610590043No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA2780.318756163001353No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA2690.3084367188754099No Hit
GCCACAACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTATAAGC2620.30041048455523195No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA2370.27174536198316784No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAGCCCTAAACCTCAACA2320.266012337468755No Hit
GCCACAACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTATAAGCA2230.255692893342812No Hit
GCCACAACTGGGATTAGATACCCCCCTAAGCTAGAGGAGCCTGTTCTATA1950.2235879560621001No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAT1880.21556172174192217No Hit
GCCACAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1850.21212190703327446No Hit
GCCACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1840.2109753021303919No Hit
GCCACAACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTA1840.2109753021303919No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTAT1520.17428394523814983No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTGGCCCTAAACCTCAACA1480.16969752562661958No Hit
GCCACAACTGGGATTAGATACCCCAGACCTAGAGGAGCCTGTTCTATAAG1410.16167129130644164No Hit
GCCACAACTGGGATTAGATACCCCTCGGCTAGAGGAGCCTGTTCTATAAG1340.15364505698626366No Hit
GCCACAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT1290.14791203247185086No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACA1240.14217900795743804No Hit
GCCACAACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1160.1330061687343775No Hit
GCCACAACTGGGATTGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1090.12497993441419955No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAACA1070.12268672460843442No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACA1040.11924690989978673No Hit
GCCACAATGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1030.11810030499690416No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC1020.1169537000940216No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA940.10778086087096109No Hit
GCCACAACTGGGATTAATACCCCACTATGCTTAGCCCTAAACCTCAACAG940.10778086087096109No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTGGG85350.0294.977666
CTGGGTT206.1547325E-8294.977638
CAACTGG85850.0294.977635
CACTGGG450.0294.977635
CTTGGAT108.453562E-4294.977638
CGGGATT206.1547325E-8294.977638
ACGGGAT206.1547325E-8294.977637
TGGGTTA206.1547325E-8294.977639
TTGGATT108.453562E-4294.977639
ACTGGGT206.1547325E-8294.977637
AACGGGA206.1547325E-8294.977636
CAACGGG206.1547325E-8294.977635
GCCACAA86850.0294.128541
ACTGGGA85650.0293.427837
CTGGGAT85650.0293.255658
TGGGATT85350.0293.24969
CACAACT88750.0286.336033
CCACAAC89050.0286.03392
ACAACTG90950.0278.761174
ACTGGAT550.0268.161477
Produced by FastQC (version 0.11.5)