FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-183-6-2-6-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-183-6-2-6-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75721
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA3675948.54531767937561No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA40165.303680617001889No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA32524.2947134876718485No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA26553.506292838182275No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA22472.9674726958175404No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA17922.366582586072556No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA15402.0337819099061027No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT14591.9268102639954572No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA13181.7406003618547032No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA8621.1383896145058834No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA7400.9772718268379974No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA6980.9218050474769219No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA6940.9165224970615813No Hit
GCCACAACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATA6830.9019954834193948No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA6780.8953922954002193No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAA6280.8293604152084626No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAACTTCG6250.8253985023969573No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAACTTCGCCT6130.8095508511509357No Hit
GCCACAACTGGGATTAGATACCCCACTGTGCCTAGCCGTAAACCTTGATA5940.7844587366780682No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA5760.7606872598090358No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT5320.70257920524029No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACCTCAACA5160.6814490035789279No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAA5160.6814490035789279No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCGTAAACCTTGATA4960.6550362515022253No Hit
GCCACAACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAACTTC4430.5850424584989633No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA4110.5427820551762391No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATG3470.45826124853079064No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTAAC3350.44241359728476903No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTACCCCTAAACTCTAATA3190.42128339562340694No Hit
GCCACAACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATA3160.41732148281190157No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATA3090.40807701958505566No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTCCTAAACCTCAACA2900.3829849051121882No Hit
GCCACAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG2790.3684578914700017No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCTGTCTCTTATAACCTAGA2620.3460070522048045No Hit
GCCACAACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAGCTCTAATA2540.33544195137412347No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTTATA2510.33148003856261804No Hit
GCCACAACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTA2000.26412752076702634No Hit
GCCACAACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAACTTC1950.25752433274785064No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA1740.2297909430673129No Hit
GCCACAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1520.20073691578293998No Hit
GCCACAACTGGGATTAGATACCCCACAATGCTTAGCCCTAAACCTCAACA1420.1875305397445887No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATC1310.17300352610240224No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC1200.1584765124602158No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACA1190.15715587485638066No Hit
GCCACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1140.150552686837205No Hit
GCCACAACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTAACTTCG1110.14659077402569962No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAACA990.13074312277967803No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATA950.1254605723643375No Hit
GCCACAACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG870.11489547153365645No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTGGA550.0295.06
CAACTGG74600.0295.05
ACACTGG255.2568794E-10295.04
CGGGATT206.147093E-8295.08
ACGGGAT206.147093E-8295.07
TGGGTTA108.449442E-4295.09
AACGGGA206.147093E-8295.06
CAACGGG206.147093E-8295.05
CTGGGTT157.21373E-6294.999978
TGGGATA303.6379788E-12294.999979
ACTGGGT157.21373E-6294.999977
AACTGGG74050.0294.80086
CTGGGAT74050.0294.004068
ACTGGGA74050.0294.004067
TGGGATT73850.0293.60199
GCCACAA75650.0293.44021
CACAACT77800.0283.814273
CCACAAC78150.0283.486882
ACAACTG79400.0277.537784
CTGGATT550.0268.181828
Produced by FastQC (version 0.11.5)