FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-182-6-5-8-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-182-6-5-8-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72237
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA2487934.440799036504835No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGA50707.018563893849413No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGA32054.4367844733308415No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGA30384.205601007793789No Hit
TGATTGACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAAGTCTCC23463.247643174550438No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGA19422.688372994448828No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAA13891.9228373271315256No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAA13681.8937663524232735No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGA13481.8660797098439856No Hit
TGATTGACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAA12551.7373368218502983No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAA12401.7165718399158325No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAA12291.7013441864972243No Hit
TGATTGACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAA12101.675041876046901No Hit
TGATTGACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCT11201.550451984440107No Hit
TGATTGACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCA11181.547683320182178No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCT9741.3483394936113071No Hit
TGATTGACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAA8791.216827941359691No Hit
TGATTGACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAAGTC7621.0548610822708584No Hit
TGATTGACACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAA7591.0507080858839652No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAA7401.024405775433642No Hit
TGATTGACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAG6870.9510361725985298No Hit
TGATTGACACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCT6660.9219651978902779No Hit
TGATTGACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTA6230.8624389163448095No Hit
TGATTGACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTAAGTCT5630.7793789886069465No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGA5170.715699710674585No Hit
TGATTGACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTC4670.646483104226366No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCCAAA4400.6091061367443277No Hit
TGATTGACACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTA4360.6035688082284701No Hit
TGATTGACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAAGTC3930.5440425266830018No Hit
TGATTGACACTGGGATTAGATACCCCGGTACCTGTCGATGAGTCTTCGGT3730.5163558841037141No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGA3670.5080498913299278No Hit
TGATTGACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAA3490.483131913008569No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGA3420.4734415881058184No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTAGCCCTAAACCTCAAC2940.40699364591552806No Hit
TGATTGACACTGGGATTAGATACCCCGTCCTAGAGGAGCCTGTTCTAAGT2790.3862286639810623No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAA2520.34885169649902403No Hit
TGATTGACACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGA2380.32947104669352273No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGA2140.29624707559837754No Hit
TGATTGACACTGGGATTAGATACCCCAGAGAGATAGAGGAGCCTGTTCTA1970.2727134294059831No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAGA1620.22426180489222972No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAA1560.21595581211844347No Hit
TGATTGACACTGGGATTAGATACCCCAGAGGAGCCTGTTCTAAGTCTCCT1450.2007281586998353No Hit
TGATTGACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC1400.19380649805501338No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGTCGTAAACCCTAA1360.18826916953915582No Hit
TGATTGACACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAA1150.15919819483090383No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCATAAACATTGA1140.15781386270193945No Hit
TGATTGACACTGGGATTAGATACCCCTATTGAGGTTTGTGAAGCCAGGGA910.12597422373575867No Hit
TGATTGACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCC890.12320555947782992No Hit
TGATTGACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAAC850.1176682309619724No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAA800.11074657031715049No Hit
TGATTGACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAAGTCTC760.10520924180129297No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGGG350.0295.000037
ACGGGAT350.0295.000039
CACGGGA350.0295.000038
AACTGGG157.2123494E-6295.07
GATTGAC71650.0295.02
GACATGG157.2123494E-6295.06
TTGACAT206.145092E-8294.999974
ATTGACC108.4486353E-4294.999973
TGATTGA72100.0294.18171
CACTGGG70650.0294.16498
GACACTG71150.0293.756166
ACACTGG71150.0293.548867
TTGACAC71650.0293.353124
ACTGGGA70950.0293.336889
TGACACT71550.0292.320075
ATTGACA72350.0291.73813
CACTGGA450.0262.222238
ACTGGAT500.0236.09
TGACACG450.0229.444445
GACACGG450.0229.444446
Produced by FastQC (version 0.11.5)