FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-181-6-2-6-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-181-6-2-6-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72070
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA3468048.11988344664909No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA785910.90467600943527No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT41525.761065630636881No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA32754.544193145552935No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA14702.039683640904676No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA13741.9064798112945747No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA13571.8828916331344525No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA13571.8828916331344525No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA12581.7455251838490355No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA12121.681698348827529No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA10551.4638545858193424No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA9631.3362009157763286No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA8901.2349105036769807No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAC8511.180796447897877No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACCTAGCTGT6750.9365894269460248No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAC4480.621617871513806No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA3790.5258776189815457No Hit
GCCACAACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATA3240.4495629249340919No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT2880.3996114888303039No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCACAACA2790.3871236298043569No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCTCTAAACTCCAATA2720.37741085056195367No Hit
GCCACAACTGGGATTAGATACCCCCACTATGCTTAGCCCTAAACCTCAAC2490.34549743305120023No Hit
GCCACAACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACCTAG2460.34133481337588456No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTACCCCTAAACTCTAATA2310.32052171499930626No Hit
GCCACAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG2190.3038712362980435No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG1680.23310670181767726No Hit
GCCACAACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAATA1620.22478146246704594No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACATTGATA1610.22339392257527402No Hit
GCCACAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1420.19703066463160815No Hit
GCCACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1310.18176772582211737No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC980.13597890939364507No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAGTA940.13042874982655753No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACA810.11239073123352297No Hit
GCCACAACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG790.10961565144997917No Hit
GCCACAACTGGGATTGATACCCCACTATGCTTAGCCCTAAACCTCAACAG790.10961565144997917No Hit
GCCACAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT770.1068405716664354No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGA108.4490137E-4294.995157
CAATGGG206.145456E-8294.995155
TAGGATT108.4490137E-4294.995159
AACCGGG108.4490137E-4294.995156
CTGGGTT108.4490137E-4294.995158
AACTGGT108.4490137E-4294.995156
AACTGGA550.0294.995156
CAACCGG108.4490137E-4294.995155
CAACTGG70250.0294.995155
CACTGGG400.0294.995155
CTAGGAT108.4490137E-4294.995158
AACTAGG108.4490137E-4294.995156
AATGGGA206.145456E-8294.995156
TGGGTTA108.4490137E-4294.995159
ACTAGGA108.4490137E-4294.995157
CCGGGAT108.4490137E-4294.995158
ACTGGGT108.4490137E-4294.995157
AACTGGG69650.0294.783366
GCCACAA71750.0293.967251
ACTGGGA69950.0293.097387
Produced by FastQC (version 0.11.5)