FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-180-6-8-1-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-180-6-8-1-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71877
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG3309346.041153637464No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG35744.9723833771582004No Hit
GGTACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAAGTCTCCTGT29894.1584929810648745No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG17902.490365485482143No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG17192.391585625443466No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC14221.978379732042239No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG13961.942206825549202No Hit
GGTACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT13031.8128191215548786No Hit
GGTACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG12871.7905588714053173No Hit
GGTACACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATAT11931.6597799017766461No Hit
GGTACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG10221.4218734783032123No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG10171.4149171501314746No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG9161.2743993210623705No Hit
GGTACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG8691.2090098362480348No Hit
GGTACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT6890.9585820220654729No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG6040.8403244431459298No Hit
GGTACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAAG5080.7067629422485635No Hit
GGTACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAAGTCTCCT4680.6511123168746609No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGATGC4460.6205044729190143No Hit
GGTACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAAGTCTCCTG4070.5662451131794594No Hit
GGTACACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTAAGTCT3710.516159550342947No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG3400.47303031567817244No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG3110.432683612282093No Hit
GGTACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT2900.40346703396079414No Hit
GGTACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAAGT2680.37285919000514767No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATTA2590.3603377992960196No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATAG2290.31859983026559263No Hit
GGTACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT2200.3060784395564645No Hit
GGTACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAAGTCTCC1980.27547059560081805No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGATAC1960.27268806433212295No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAACAG1960.27268806433212295No Hit
GGTACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAAGTCTC1790.24903654854821433No Hit
GGTACACTGGGATTAGATACCCCTCGTCGGCAGCGTCAGATGTGTATAGA1650.2295588296673484No Hit
GGTACACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTAAGTCTCC1630.22677629839865324No Hit
GGTACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAAGTCTCC1620.22538503276430566No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATAG1520.21147237642083003No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATCGATTG1500.20868984515213493No Hit
GGTACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT1470.2045160482490922No Hit
GGTACACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAATAG1390.19338592317431166No Hit
GGTACACTGGGATTAGATACCCCACGAGACAGAGGAGCCTGTTCTAAGTC1290.179473266830836No Hit
GGTACACTGGGATTAGATACCCCCCCTAGAGGAGCCTGTTCTAAGTCTCC1290.179473266830836No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCGTAAACCCAGACGT1250.17390820429344575No Hit
GGTACACTGGGATTAGATACCCCGTCTCGTCTAGAGGAGCCTGTTCTAAG1230.17112567302475062No Hit
GGTACACTGGGATTAGATACCCCCCCGTCCTAGAGGAGCCTGTTCTAAGT1220.16973440739040305No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG1210.1683431417560555No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAGATAG1200.16695187612170792No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACAG1190.16556061048736034No Hit
GGTACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTAG1050.14608289160649443No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACAG980.13634403216606147No Hit
GGTACACTGGGATTAGATACCCCTGACGCTAAGAGGAGCCTGTTCTAAGT970.1349527665317139No Hit
GGTACACTGGGATTAGATACCCCACCATGCTTAGCCCTAAACCTCAACAG910.1266051727256285No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCCAAACCTCAACAG900.12521390709128094No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAACAG900.12521390709128094No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATAG860.11964884455389066No Hit
GGTACACTGGGATTAGATACCCCGCACTAGAGGAGCCTGTTCTAAGTCTC860.11964884455389066No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGACAG850.11825757891954311No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTACCCCTAAACTCTAATAG840.11686631328519555No Hit
GGTACACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT830.11547504765084798No Hit
GGTACACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATAA830.11547504765084798No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAGTAG800.1113012507478053No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAGATAA790.10990998511345772No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAACAG780.10851871947911015No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAAG780.10851871947911015No Hit
GGTACACTGGGATTAGATACCCCGTCTCTCTAGAGGAGCCTGTTCTAAGT780.10851871947911015No Hit
GGTACATGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT750.10434492257606746No Hit
GGTACACTGGGATTAGATACCCCTCTATACTAAATTACTTTATCCTATTA750.10434492257606746No Hit
GGTACACTGGGATTAGATACCCCCTCGTCGGCAGCGTCAGATGTGTAGAG740.10295365694171989No Hit
GGTACACTGGGATTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT740.10295365694171989No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAA730.10156239130737232No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACGG720.10017112567302475No Hit
GGTACACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATAA720.10017112567302475No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTGGG70100.0294.98545
ACCGGGA108.4498024E-4294.985386
ATGGGAT157.2136318E-6294.985386
ACACGGG255.2568794E-10294.985384
TGGTATT108.4498024E-4294.985388
ACATGGG157.2136318E-6294.985384
CTGGGTT108.4498024E-4294.985387
GTACTGG108.4498024E-4294.985382
CACCGGG108.4498024E-4294.985385
GTACATT108.4498024E-4294.985382
CACTGGT157.2136318E-6294.985385
CTGGATT400.0294.985387
TGGATTA400.0294.985388
TACTGGG108.4498024E-4294.985383
ACGGGAT255.2568794E-10294.985386
GGGATAG255.2568794E-10294.985389
CATGGGA157.2136318E-6294.985385
CCGGGAT108.4498024E-4294.985387
TGGGATA255.2568794E-10294.985388
ACTGGGT108.4498024E-4294.985386
Produced by FastQC (version 0.11.5)