FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-18-5-4-1-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-18-5-4-1-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11784
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG357330.320773930753564No Hit
GGTACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTATAAGCACTGT8787.450780719619823No Hit
GGTACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTATA7976.763408010862186No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG6365.39714867617108No Hit
GGTACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTATAAGCAC3492.961642905634759No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC2602.2063815342837745No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTATA2572.1809232858112697No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG2271.9263408010862186No Hit
GGTACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTATAAGCAC2241.9008825526137134No Hit
GGTACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT2171.8414799728445348No Hit
GGTACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTATAAGCACTG1751.4850644942294635No Hit
GGTACACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTATAAGCACT1721.4596062457569587No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT1561.3238289205702647No Hit
GGTACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTATAAGCA1461.238968092328581No Hit
GGTACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTATAAGCA1351.145621181262729No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCTAGAGGAGCCTGTTCTATA1281.0862186014935504No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG1211.026816021724372No Hit
GGTACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT820.6958587915818059No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG750.6364562118126273No Hit
GGTACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG730.6194840461642905No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTATA710.6025118805159538No Hit
GGTACACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTATAA660.560081466395112No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG620.5261371350984385No Hit
GGTACACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTATAA520.44127630685675495No Hit
GGTACACTGGGATTAGATACCCCATACGACTCACTAGAGGAGCCTGTTCT500.4243041412084182No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG500.4243041412084182No Hit
GGTACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAT500.4243041412084182No Hit
GGTACACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTAT450.3818737270875764No Hit
GGTACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG450.3818737270875764No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTAT440.37338764426340804No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG430.3649015614392397No Hit
GGTACACTGGGATTAGATACCCCGTGTAGTCTAGAGGAGCCTGTTCTATA400.3394433129667346No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG400.3394433129667346No Hit
GGTACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG350.2970128988458927No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG340.28852681602172436No Hit
GGTACACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTATAAGCA340.28852681602172436No Hit
GGTACACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTATAAGC340.28852681602172436No Hit
GGTACACTGGGATTAGATACCCCACGCTAGAGGAGCCTGTTCTATAAGCA330.280040733197556No Hit
GGTACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTATAAGCACT330.280040733197556No Hit
GGTACACTGGGATTAGATACCCCGAGCTAGAGGAGCCTGTTCTATAAGCA320.2715546503733876No Hit
GGTACACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTATAAG300.2545824847250509No Hit
GGTACACTGGGATTAGATACCCCACAGACCTAGAGGAGCCTGTTCTATAA280.23761031907671418No Hit
GGTACACTGGGATTAGATACCCCCGACTAGAGGAGCCTGTTCTATAAGCA270.22912423625254583No Hit
GGTACACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTATAAGC270.22912423625254583No Hit
GGTACACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGCC270.22912423625254583No Hit
GGTACACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTATAAGCAC260.22063815342837748No Hit
GGTACACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGCCTG260.22063815342837748No Hit
GGTACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT240.20366598778004072No Hit
GGTACACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTATAA240.20366598778004072No Hit
GGTACACTGGGATTAGATACCCCGAGTAGTCTAGAGGAGCCTGTTCTATA240.20366598778004072No Hit
GGTACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTATAA230.19517990495587237No Hit
GGTACACTGGGATTAGATACCCCCCACCTAGAGGAGCCTGTTCTATAAGC220.18669382213170402No Hit
GGTACACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTATAAGCAC220.18669382213170402No Hit
GGTACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT220.18669382213170402No Hit
GGTACACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTATAAGCAC220.18669382213170402No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG210.17820773930753564No Hit
GGTACACTGGGATTAGATACCCCTGACGCTAGAGGAGCCTGTTCTATAAG210.17820773930753564No Hit
GGTACACTGGGATTAGATACCCCACGTCTAGAGGAGCCTGTTCTATAAGC200.1697216564833673No Hit
GGTACACTGGGATTAGATACCCCGGGTAGTCCTAGAGGAGCCTGTTCTAT190.1612355736591989No Hit
GGTACACTGGGATTAGATACCCCCGTGGACTAGAGGAGCCTGTTCTATAA180.15274949083503053No Hit
GGTACACTGGGATTAGATACCCCCCGACTAGAGGAGCCTGTTCTATAAGC180.15274949083503053No Hit
GGTACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT170.14426340801086218No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAT170.14426340801086218No Hit
GGTACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTAG170.14426340801086218No Hit
GGTACACTGGGATTAGATACCCCACTGACACTAGAGGAGCCTGTTCTATA170.14426340801086218No Hit
GGTACACTGGGATTAGATACCCCACGAGATCTAGAGGAGCCTGTTCTATA160.1357773251866938No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTCAACAG160.1357773251866938No Hit
GGTACACTGGGATTAGATACCCCCACGAACCTAGAGGAGCCTGTTCTATA160.1357773251866938No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG140.11880515953835709No Hit
GGTACACTGGGATTAGATACCCCTGACCGACGACTAGAGGAGCCTGTTCT140.11880515953835709No Hit
GGTACACTGGGATTAGATACCCCATTCTCGTTCTAGAGGAGCCTGTTCTA140.11880515953835709No Hit
GGTACACTGGGATTAGATACCCCTATAAGAGGAGCCTGTTCTATAAGCAC130.11031907671418874No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATAG130.11031907671418874No Hit
GGTACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT130.11031907671418874No Hit
GGTACACTGGGATTAGATACCCCGGGTAGCTAGAGGAGCCTGTTCTATAA130.11031907671418874No Hit
GGTACACTGGGATTAGATACCCCTGTATAAGAGGAGCCTGTTCTATAAGC120.10183299389002036No Hit
GGTACACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTATAAGCAC120.10183299389002036No Hit
GGTACACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT120.10183299389002036No Hit
GGTACACTGGGATTAGATACCCCCGAGCTAGAGGAGCCTGTTCTATAAGC120.10183299389002036No Hit
GGTACACTGGGATTAGATACCCCGCCTAGAGGAGCCTGTTCTATAAGCAC120.10183299389002036No Hit
GGTACACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTATAAGCACT120.10183299389002036No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAACAG120.10183299389002036No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGGG108.358896E-4295.04
CTGGGAT11400.0295.07
ACACTGG11600.0295.04
TACACGG108.358896E-4295.03
ACGGGAT108.358896E-4295.06
TGGGATT11350.0295.08
ACTGGGA11450.0295.06
CACGGGA108.358896E-4295.05
CACTGGG11500.0294.999975
GGTACAC11800.0293.751
GGGATTA11350.0293.700449
GTACACT11800.0291.252
TACACTG11800.0291.253
CACTGGA150.0028140582196.666675
GTACACG200.0066536483147.52
ACACGAA650.00242387368.07693295
CAGCCCT150.00435987759.00000430-34
AACGCTT257.742468E-659.00000490-94
AATCTTG257.742468E-659.00000440-44
AACCGCT600.059.00000460-64
Produced by FastQC (version 0.11.5)