FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-179-6-3-8-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-179-6-3-8-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68852
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA3031344.02631731830593No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGA40095.82263405565561No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGA24273.5249520711090456No Hit
TGATTGACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCA22583.2794980537965492No Hit
TGATTGACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTATAAGCAC21843.172021146807645No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAA18982.756637425201882No Hit
TGATTGACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAA15612.2671817812118746No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAA10701.5540579794341485No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAA9681.4059141346656596No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAA9081.3187706965665487No Hit
TGATTGACACTGGGATTAGATACCCCTCGTCGGCAGCGTCAGATGTGTAG7541.09510253877883No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAA7411.0762214605240226No Hit
TGATTGACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTATAAGC7301.0602451635391854No Hit
TGATTGACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTATAAG7291.0587927729042002No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGA7221.048626038459304No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGA7001.01667344448963No Hit
TGATTGACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAA6690.9716493348050892No Hit
TGATTGACACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTATA5650.8206007087666299No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTA4970.7218381455876373No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAA4190.6085516760587928No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATA3860.5606227851042817No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAA3850.5591703944692964No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCTAAACCCAGAT3630.5272178004996224No Hit
TGATTGACACTGGGATTAGATACCCCTGACTAGAGGAGCCTGTTCTATAA3440.4996223784349038No Hit
TGATTGACACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTATAA3110.45169348748039273No Hit
TGATTGACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCC2900.42119328414570384No Hit
TGATTGACACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTATAAG2850.4139313309707779No Hit
TGATTGACACTGGGATTAGATACCCCGTTTCGTGGGCTAGAGGAGCCTGT2600.37762156509614825No Hit
TGATTGACACTGGGATTAGATACCCCCTATGCTTAGCCCTAAACTCTAAT2570.3732643931911927No Hit
TGATTGACACTGGGATTAGATACCCCTCGTCGCAGCGTCAGATGTGTAGA2550.37035961192122235No Hit
TGATTGACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCC2460.35728809620635565No Hit
TGATTGACACTGGGATTAGATACCCCCGTCTCGTCTAGAGGAGCCTGTTC2240.32533550223668156No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAACCCTAAACTCGAA2200.3195259396967408No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCT2140.3108115958868297No Hit
TGATTGACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCT2020.2933829082670075No Hit
TGATTGACACTGGGATTAGATACCCCGTCTAGTGGGATTAGAGGAGCCTG1900.2759542206471853No Hit
TGATTGACACTGGGATTAGATACCCCTTCTAGAGGAGCCTGTTCTATAAG1890.2745018300122001No Hit
TGATTGACACTGGGATTAGATACCCCTGTTAGAGGAGCCTGTTCTATAAG1770.2570731423923779No Hit
TGATTGACACTGGGATTAGATACCCCATGTGCCTCTAGAGGAGCCTGTTC1730.25126357985243714No Hit
TGATTGACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAG1690.2454540173124964No Hit
TGATTGACACTGGGATTAGATACCCCTGAGGAGGAGCCTGTTCTATAAGC1680.24400162667751116No Hit
TGATTGACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC1680.24400162667751116No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGA1670.24254923604252598No Hit
TGATTGACACTGGGATTAGATACCCCGTTCGTGGCTAGAGGAGCCTGTTC1590.2309301109626445No Hit
TGATTGACACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATC1580.22947772032765934No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAGA1540.22366815778771856No Hit
TGATTGACACTGGGATTAGATACCCCGTCTCGTGGTCTAGAGGAGCCTGT1520.22076337651774822No Hit
TGATTGACACTGGGATTAGATACCCCCACGATAGAGGAGCCTGTTCTATA1520.22076337651774822No Hit
TGATTGACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTATAAGCA1460.2120490327078371No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAGCCTCAA1400.20333468889792597No Hit
TGATTGACACTGGGATTAGATACCCCTCACTAGAGGAGCCTGTTCTATAA1390.2018822982629408No Hit
TGATTGACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTATAA1380.20042990762795562No Hit
TGATTGACACTGGGATTAGATACCCCTCGGCCTAGAGGAGCCTGTTCTAT1360.19752512635798522No Hit
TGATTGACACTGGGATTAGATACCCCACAGATAGAGGAGCCTGTTCTATA1360.19752512635798522No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACAGAGGAGCCTGTTCTAT1290.18735839191308895No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACGAGGAGCCTGTTCTATA1260.18300122000813338No Hit
TGATTGACACTGGGATTAGATACCCCGAAATTAATACGACTCACTAGAGG1200.17428687619822228No Hit
TGATTGACACTGGGATTAGATACCCCCCCTAGAGGAGCCTGTTCTATAAG1180.1713820949282519No Hit
TGATTGACACTGGGATTAGATACCCCTGTCTCGAGGAGCCTGTTCTATAA1080.15685818857840006No Hit
TGATTGACACTGGGATTAGATACCCCGTGGGCTAGAGGAGCCTGTTCTAT1060.15395340730842969No Hit
TGATTGACACTGGGATTAGATACCCCCCAACTAGAGGAGCCTGTTCTATA1030.1495962354034741No Hit
TGATTGACACTGGGATTAGATACCCCCTGCTAGAGGAGCCTGTTCTATAA1030.1495962354034741No Hit
TGATTGACACTGGGATTAGATACCCCCTGTGGCTAGAGGAGCCTGTTCTA1010.14669145413350376No Hit
TGATTGACACTGGGATTAGATACCCCCTCCTCTAGAGGAGCCTGTTCTAT930.13507232905362226No Hit
TGATTGACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAAC860.12490559460872595No Hit
TGATTGACACTGGGATTAGATACCCCAGGCTCCTCTTCGTCGGCAGCGTC820.11909603206878522No Hit
TGATTGACACTGGGATTAGATACCCCGTATAGAGGAGCCTGTTCTATAAG820.11909603206878522No Hit
TGATTGACACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAA780.11328646952884448No Hit
TGATTGACACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTATAAGC770.11183407889385928No Hit
TGATTGACACTGGGATTAGATACCCCGTCTCGTGGTCTCTAGAGGAGCCT760.11038168825887411No Hit
TGATTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC750.10892929762388892No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTATAA740.10747690698890375No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTACCCCTAAACTCTAA740.10747690698890375No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGGG157.213379E-6294.974557
TTGCACT157.213379E-6294.974554
GATTGCA157.213379E-6294.974552
ACGGGAT157.213379E-6294.974559
TTGACAC68150.0294.541754
GACACTG67850.0294.539836
GATTGAC68300.0294.326752
ACACTGG68100.0294.324867
TGACACT68100.0293.891725
TGATTGA68550.0293.683651
CACTGGG67950.0293.672248
ACTGGGA68050.0293.0249
ATTGACA68600.0292.824653
CACTGGA400.0258.102788
GACACGG251.8702485E-7235.979686
TGCACTG202.273629E-5221.230945
TGATTGC202.273629E-5221.230941
ATTGCAC202.273629E-5221.230943
ACTGGAT457.6397555E-11196.649729
AACTGGG150.0028446398196.64977
Produced by FastQC (version 0.11.5)