FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-178-6-1-2-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-178-6-1-2-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62244
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA2446539.304993252361676No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA34785.5876871666345345No Hit
CAACACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAAGTCTCCTG25824.1481909902962535No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA19743.1713900134952766No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA15282.454855086434034No Hit
CAACACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT15032.414690572585309No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA12171.9552085341559025No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA11801.8957650536597905No Hit
CAACACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA11591.862026862026862No Hit
CAACACACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATA10441.67727009832273No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA8591.3800526958421695No Hit
CAACACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA8411.351134245871088No Hit
CAACACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA7561.214574898785425No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA5650.9077180129811709No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA5220.838635049161365No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA5080.8161429214060792No Hit
CAACACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA4640.7454533770323244No Hit
CAACACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAAGTCTCCT4620.7422402159244265No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA4240.6811901548743654No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGATA4080.6554848660111818No Hit
CAACACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT3610.57997557997558No Hit
CAACACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAA3540.5687295160979372No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGATG3490.5606966133281922No Hit
CAACACACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTAAGTC3250.5221386800334169No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATA3210.5157123578176209No Hit
CAACACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAAGTCTCC3100.49803997172418224No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTTGGTA2860.4594820384294068No Hit
CAACACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAAG2630.42253068568858043No Hit
CAACACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAAGTCT2530.40646488014909066No Hit
CAACACACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTAAGTCTC2390.383972752393805No Hit
CAACACGCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA1980.31810294968189706No Hit
CAACACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA1960.3148897885739991No Hit
CAACACACTGGGATTAGATACCCCACGAGACAGAGGAGCCTGTTCTAAGT1920.3084634663582032No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAACA1910.30685688580425424No Hit
CAACACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTA1850.2972174024805604No Hit
CAACACACTGGGATTAGATACCCCACTATGCTCAGCCGTAAACCCAGACG1840.2956108219266114No Hit
CAACACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1760.2827581774950196No Hit
CAACACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAAGTCTC1650.2650857914015809No Hit
CAACACACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATA1640.26347921084763193No Hit
CAACACACTGGGATTAGATACCCCACGAGACGTCTAGAGGAGCCTGTTCT1540.24741340530814215No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTACCCCTAAACTCTAATA1490.2393805025383973No Hit
CAACACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAACA1470.23616734143049933No Hit
CAACACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA1440.23134759976865238No Hit
CAACACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAAGTCTC1370.2201015358910096No Hit
CAACACACTGGGATTAGATACCCCCCCTAGAGGAGCCTGTTCTAAGTCTC1340.21528179422916266No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT1320.21206863312126467No Hit
CAACACACTGGGATTAGACACCCCACTATGCTTAGCCCTAAACCTCAACA1290.20724889145941777No Hit
CAACACACTGGGATTAGATACCCCGCACTAGAGGAGCCTGTTCTAAGTCT1200.192789666473877No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATA1080.1735106998264893No Hit
CAACACACTGGGATTAGATACCCCGTCTCGTCTAGAGGAGCCTGTTCTAA1020.16387121650279546No Hit
CAACACACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTAAGTCTCC970.15583831373305057No Hit
CAACACACTGGGATTAGATACCCCGTCTCTCTAGAGGAGCCTGTTCTAAG930.14941199151725468No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACA920.1478054109633057No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAGTA860.13816592763961186No Hit
CAACACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG850.13655934708566286No Hit
CAACACACTGGGATTAGATACCCCACGAGAGGAGCCTGTTCTAAGTCTCC800.128526444315918No Hit
CAACACACTGGGATTAGATACCCCCTATGCTTAGCCCTAAACTCTAATAG750.12049354154617313No Hit
CAACACACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAATA740.11888696099222415No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTAGCCCCAAACCTCAACA720.11567379988432619No Hit
CAACACCCTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA720.11567379988432619No Hit
CAACACACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGC700.11246063877642824No Hit
CAACACACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAACA700.11246063877642824No Hit
CAACACACTGGGATTAGATACCCCAGAAAGAGAGGTGCGTGCGCGCGTGT690.11085405822247928No Hit
CAACACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAAGTCTC680.10924747766853031No Hit
CAACACACTGGGATTAGATACCCCCGAGCTAGAGGAGCCTGTTCTAAGTC670.10764089711458133No Hit
CAACACACTGGGATTAGATACCCCGAGTACTCCGGCTTGAGGAATGCGTC650.10442773600668337No Hit
CAACACACTGGGATTAGATACCCCTCTATACTAAATTACTTTATCCTATT640.1028211554527344No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTGG157.207549E-6295.05
ACACGGG303.6379788E-12295.05
TAGGATT108.4458233E-4295.09
CTGGGTT157.207549E-6295.08
GCTGGGA550.0295.07
CACTGGG59850.0295.06
CTAGGAT108.4458233E-4295.08
CACCCTG206.1383616E-8295.04
GTGGGAT157.207549E-6295.08
CGGGATT255.2568794E-10295.08
CCCTGGG206.1383616E-8295.06
ACCCTGG206.1383616E-8295.05
ACGGGAT303.6379788E-12295.07
TGGGTTA108.4458233E-4295.09
ACTGGAT500.0295.07
CAGTGGG157.207549E-6295.06
CACGGGA303.6379788E-12295.06
ACACTGG60450.0294.755985
ACTGGGA59950.0293.76987
TGGGATT60600.0293.539589
Produced by FastQC (version 0.11.5)