FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-177-6-5-7-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-177-6-5-7-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61330
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC2288437.31289744007826No Hit
CTGGATGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAAGTCTCCT38006.195988912440894No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT27424.470895157345508No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT27404.467634110549486No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT23683.861079406489483No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT18092.9496168270014675No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTA17862.91211478884722No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA13032.124571987608022No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT9851.6060655470406No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT7471.2180009783140389No Hit
CTGGATGACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTA7271.1853905103538236No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC6831.1136474808413501No Hit
CTGGATGACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTAAGTCT6671.087559106473178No Hit
CTGGATGACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTAAGT6431.0484265449209196No Hit
CTGGATGACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCT6231.0158160769607045No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC6020.9815750856024784No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATAT5690.9277678134681232No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT5690.9277678134681232No Hit
CTGGATGACTGGGATTAGATACCCCTTCTAGAGGAGCCTGTTCTAAGTCT5450.888635251915865No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT5400.8804826349258112No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGAT3810.6212294146421001No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAGAT3720.6065547040600033No Hit
CTGGATGACTGGGATTAGATACCCCGCCAGACCTAGAGGAGCCTGTTCTA3610.588618946681885No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAGAT3190.520136963965433No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT2970.4842654492091962No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTCGCCCTAAACCTCAAC2930.4777433556171531No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAAC2910.4744823088211315No Hit
CTGGATGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC2840.46306864503505624No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGAC2810.458177074841024No Hit
CTGGATGACTGGGATTAGATACCCCCTATGCTTAGCCCTAAACTCTAATA2740.4467634110549486No Hit
CTGGATGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT2490.40600032610467957No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAAC2480.40436980270666883No Hit
CTGGATGACTGGGATTAGATACCCCGGACCTAGAGGAGCCTGTTCTAAGT2410.39295613892059356No Hit
CTGGATGACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTA2330.3799119517365074No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTAGAGGAGCCTG2260.3684982879504321No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGAT2210.3603456709603783No Hit
CTGGATGACTGGGATTAGATACCCCACACCTAGAGGAGCCTGTTCTAAGT2110.34404043698027065No Hit
CTGGATGACTGGGATTAGATACCCCACTATTCTTAGCCCTAAACCTCAAC2020.3293657263981738No Hit
CTGGATGACTGGGATTAGATACCCCATTATGCTTAGCCCTAAACCTCAAC2000.3261046796021523No Hit
CTGGATGACTGGGATTAGATACCCCGACGAGGAGCCTGTTCTAAGTCTCC1820.2967552584379586No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAAT1800.29349421164193706No Hit
CTGGATGACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCT1550.25273112669166803No Hit
CTGGATGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA1480.2413174629055927No Hit
CTGGATGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT1460.23805641610957118No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT1440.23479536931354963No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGAT1390.22664275232349584No Hit
CTGGATGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCA1030.16794390999510844No Hit
CTGGATGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACA990.16142181640306538No Hit
CTGGATGACTGGGATTAGATACCCCCGCCTAGAGGAGCCTGTTCTAAGTC940.15326919941301156No Hit
CTGGATGACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAAGTC900.14674710582096853No Hit
CTGGATGACTGGGATTAGATACCCCGTCGGCGCGTCAGAGGAGCCTGTTC900.14674710582096853No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTGGCCGTAAACTTTGAT830.1353334420348932No Hit
CTGGATGACTGGGATTAGATACCCCAGCTAATGCGAGGAGCCTGTTCTAA800.13044187184086092No Hit
CTGGATGACTGGGATTAGATACCCCAGGTATTAAAGGATTTGGAATAGTG780.1271808250448394No Hit
CTGGATGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG750.12228925485080712No Hit
CTGGATGACTGGGATTAGATACCCCCCGTTGCCGTTCGCGCAGCCGCGCC700.11413663786075331No Hit
CTGGATGACTGGGATTAGATACCCCATCCGGGAAACTGCAGTGGAAGCTA700.11413663786075331No Hit
CTGGATGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAAGTCT650.10598402087069948No Hit
CTGGATGACTGGGATTAGATACCCCCTCGTGGCTGAGATGTGTATAAGAG620.10109245067666721No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTGG206.1387254E-8294.98865
GGATACT108.446505E-4294.98863
TGGATGA60700.0294.98862
TGGATAC108.446505E-4294.98862
GACTGGT108.446505E-4294.98867
CTGGATG60800.0294.98861
TGACTGG60200.0294.98866
GACTAGG157.2081566E-6294.98867
GGATGAC60650.0294.745363
GATGACT60600.0294.258424
GACTGGG59900.0294.003667
ATGACTG60500.0293.769655
ACTGGGA60100.0293.270668
CTGGGAT60200.0293.028539
GACTGGA500.0265.489727
ACTGGAT500.0265.489728
CTGGATT450.0262.212079
GATACTG150.0028434775196.659064
TGGAGAC304.635458E-7196.659062
GGAGACT304.635458E-7196.659063
Produced by FastQC (version 0.11.5)