FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-176-6-4-6-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-176-6-4-6-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61193
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA2805345.843478829277856No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA25274.12955730230582No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA17202.810779010671155No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT16922.765022143055578No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATA16852.753582926151684No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAC14242.3270635530207704No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACAGATCTGT13862.264964946971059No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA12412.028009739676107No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA11811.929959309071299No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA10821.7681760985733663No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA9811.6031245403886067No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT9651.5769777588939913No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA7471.2207278610298564No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA6651.0867256058699526No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA6090.9952118706387986No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTATAATA5300.866112137009135No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAC4760.777866749464808No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACA4690.7664275325609139No Hit
GCCACAACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTA4080.6667429281126926No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAAGTCGAATA4070.6651087542692792No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACAGATC4060.6634745804258657No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAGCCCTAAACCTCAACA3990.6520353635219714No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA3930.6422303204614908No Hit
GCCACAACTGGGATTAGATACCCCCGAGCAGATTAATGAGGATCCCTATG3790.6193518866537022No Hit
GCCACAACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTACAGATCT3330.544179889856683No Hit
GCCACAACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATA3200.5229356298923079No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATA3140.5131305868318271No Hit
GCCACAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT3140.5131305868318271No Hit
GCCACAACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTACA2680.4379585900348079No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACG2660.434690242347981No Hit
GCCACAACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACAGATC2630.42978772081774064No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCCAAATA2550.41671433007043285No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTAACA2460.4020067654797117No Hit
GCCACAACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACAGATCTG2390.3905675485758175No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA2290.3742258101416829No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAACA2270.37095746245485595No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTACAGATCT2100.34317650711682707No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCATAAACATTGACA2010.3284689425261059No Hit
GCCACAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1950.3186638994656251No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG1460.23858938113836553No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA1450.23695520729495206No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCTAC1440.23532103345153854No Hit
GCCACAACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC1380.2255159903910578No Hit
GCCACAACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTACAGATC1370.22388181654764433No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTTATG1320.21571094733057705No Hit
GCCACAACTGGGATTAGATACCCCGTCTCGTCTAGAGGAGCCTGTTCTAC1320.21571094733057705No Hit
GCCACAACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTACAGA1290.21080842580033665No Hit
GCCACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1160.18956416583596164No Hit
GCCACAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1150.18792999199254815No Hit
GCCACAACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACAGAT1130.18466164430572124No Hit
GCCACAACTGGGATTAGATACCCCTGACTAGAGGAGCCTGTTCTACAGAT1120.18302747046230777No Hit
GCCACAACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTACAGATCT1100.17975912277548087No Hit
GCCACAACTGGGATTAGATACCCCGTGTAGTCCCTAGAGGAGCCTGTTCT1000.16341738434134623No Hit
GCCACAACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTACAGATC980.1601490366545193No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA950.15524651512427892No Hit
GCCACAACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACAGAT910.14870981975062508No Hit
GCCACAACTGGGATTAGATACCCCGTCTCTCTAGAGGAGCCTGTTCTACA840.13727060284673084No Hit
GCCACAACTGGGATTAGATACCCCAGAGACCTAGAGGAGCCTGTTCTACA840.13727060284673084No Hit
GCCACAACTGGGATTAGATACCCCTGAGCCCACGAGACCTAGAGGAGCCT780.12746555978625007No Hit
GCCACAACTGGGATTAGATACCCCCCACTAGAGGAGCCTGTTCTACAGAT760.12419721209942314No Hit
GCCACAACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG670.10948964750870198No Hit
GCCACAACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTACAGATC670.10948964750870198No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCTAGAGGAGCCTGTTCTACA660.10785547366528853No Hit
GCCACAACTGGGATTAGATACCCCAAGAGACCTAGAGGAGCCTGTTCTAC660.10785547366528853No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGG157.2080747E-6294.988535
CCACTGG157.2080747E-6294.988535
CTGGATT303.6379788E-12294.988538
TGGGATA157.2080747E-6294.988539
ACTGGAT303.6379788E-12294.988537
AACGGGA157.2080747E-6294.988536
CAACGGG157.2080747E-6294.988535
CAACTGG59900.0294.742345
AACTGGG59650.0294.494026
GCCACAA60800.0293.77561
CTGGGAT60000.0293.26788
ACTGGGA60000.0293.26787
TGGGATT60000.0292.53039
CACAACT62150.0284.783783
CCACAAC62500.0284.604952
ACAACTG62250.0284.08944
AACTGGA351.2732926E-11252.847326
ATGGGAT202.2719592E-5221.241417
ACCACTG202.2719592E-5221.241414
ACACTGG403.45608E-11221.241414
Produced by FastQC (version 0.11.5)