FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-175-6-7-5-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-175-6-7-5-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56534
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG3763366.56702161531113No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG18653.29889977712527No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC14062.486998974068702No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG10411.8413697951675099No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG9701.7157816535182366No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG9371.6574097003573072No Hit
AAGCGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG8941.5813492765415504No Hit
AAGCGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG6981.2346552517069374No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG5731.0135493685215977No Hit
AAGCGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG5721.0117805214561149No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG5570.9852478154738741No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATAT5490.9710970389500124No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG5460.9657904977535643No Hit
AAGCGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT4680.8278204266459122No Hit
AAGCGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT4520.7995188735981886No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATAG3930.6951568967347084No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTCG2570.4545936958290586No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATTA2430.42982983691230053No Hit
AAGCGACTGGGATTAGATACCCCGAGAGTTTTCGGAAGATCGTGGCCCTC2430.42982983691230053No Hit
AAGCGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT1900.3360809424417165No Hit
AAGCGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG1710.30247284819754483No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG1290.22818127144727066No Hit
AAGCGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT1290.22818127144727066No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTAATGC1150.20341741253051263No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTAACAG1080.19103548307213358No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAACAG1010.17865355361375454No Hit
AAGCGACTGGGATTAGATACCCCACTATGCCTACCCGTAAACCTTGATAG1000.17688470654827185No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACAG1000.17688470654827185No Hit
AAGCGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT980.1733470124173064No Hit
AAGCGACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATAA890.15742738882796192No Hit
AAGCGACTGGGATTAGATACCCCACCATGCTTAGCCCTAAACCTCAACAG820.1450454593695829No Hit
AAGCGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACCTAGCTGTC800.14150776523861747No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACAG760.1344323769766866No Hit
AAGCGACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCTCG750.1326635299112039No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTTACCCCTAAACTCTAATAG710.125588141649273No Hit
AAGCGACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT620.10966851805992853No Hit
AAGCGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG600.1061308239289631No Hit
AAGCGATGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT580.10259312979799767No Hit
AAGCGACTGGGATTAATACCCCACTATGCTTAGCCCTAAACCTCAACAGT580.10259312979799767No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTGAT157.2052626E-6294.987647
TGGATTA157.2052626E-6294.987648
ACTGGGA55300.0294.987646
TGTGATT157.2052626E-6294.987648
GGGTTAG108.444839E-4294.98769
TGGTATT108.444839E-4294.98768
GCGACTG55850.0294.98763
GACGGGA206.134542E-8294.98765
CTGGTAT108.444839E-4294.98767
ACGGGAT206.134542E-8294.98766
GACTGGT108.444839E-4294.98765
GACTGGG55450.0294.98765
AAGCGAC56300.0294.98761
GGGATAG108.444839E-4294.98769
TGGGTTA108.444839E-4294.98768
CGACGGG206.134542E-8294.98764
TGGGATA108.444839E-4294.98768
ACTGGTA108.444839E-4294.98766
ACTGGGT108.444839E-4294.98766
AGCGACT55950.0294.98762
Produced by FastQC (version 0.11.5)