FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-174-6-4-8-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-174-6-4-8-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56032
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA2681947.86372073101085No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGA31145.557538549400343No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGA18433.2891918903483726No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGA14152.525342661336379No Hit
TGATTGACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTATTCCTCT12852.293332381496288No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAA12662.2594231867504284No Hit
TGATTGACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCA10701.909623072529983No Hit
TGATTGACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAA9801.7490005711022272No Hit
TGATTGACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAA9391.6758280982295832No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAA8941.5955168475157055No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGA8671.5473300970873787No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAA7861.4027698458023987No Hit
TGATTGACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAA7181.2814106225014277No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAA5711.0190605368360937No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAA5711.0190605368360937No Hit
TGATTGACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCC4760.8495145631067961No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGA4440.7924043403769275No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAA3950.7049543118218161No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGA3920.6996002284408909No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCT3210.5728869217589949No Hit
TGATTGACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAA2620.4675899486007996No Hit
TGATTGACACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTATT2350.41940319817247285No Hit
TGATTGACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTATTCCT2090.3730011422044546No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATA2020.3605082809822958No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTA1830.32659908623643635No Hit
TGATTGACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC1810.32302969731581954No Hit
TGATTGACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAG1810.32302969731581954No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGA1710.30518275271273554No Hit
TGATTGACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTATTCCTC1700.30339805825242716No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAA1660.2962592804111936No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTACCCCTAAACTCTAA1590.28376641918903484No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAA1530.27305825242718446No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAA1490.2659194745859509No Hit
TGATTGACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTATTC1350.24093375214163334No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAGCCGTAAACCCAGA1200.2141633352370074No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAA1170.20880925185608223No Hit
TGATTGACACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTATTCC1060.18917761279268988No Hit
TGATTGACACTGGGATTAGATACCCCCCCGTCCTAGAGGAGCCTGTTCTA1020.1820388349514563No Hit
TGATTGACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAGCTCTAA1020.1820388349514563No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAA1000.17846944603083953No Hit
TGATTGACACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAA950.16954597372929756No Hit
TGATTGACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAAC920.16419189034837237No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATCGA900.16062250142775555No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAA880.15705311250713877No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGA870.1552684180468304No Hit
TGATTGACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCT870.1552684180468304No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAG850.15169902912621358No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAA810.14456025128498No Hit
TGATTGACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTATTCC770.13742147344374644No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGA750.13385208452312963No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAGCCCTAAACCTCAA750.13385208452312963No Hit
TGATTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC730.13028269560251285No Hit
TGATTGACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTATTCC720.12849800114220444No Hit
TGATTGACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTC690.12314391776127927No Hit
TGATTGACACTGGGATTAGATACCCCACCATGCTTAGCCCTAAACCTCAA680.12135922330097086No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAGA670.11957452884066247No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAA670.11957452884066247No Hit
TGATTGACACTGGGATTAGATACCCCGCACTAGAGGAGCCTGTTCTATTC660.11778983438035409No Hit
TGATTGACACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAA650.11600513992004569No Hit
TGATTGACACTGGGATTAGATACCCCTCTATACTAAATTACTTTATCCTA630.1124357509994289No Hit
TGATTGACACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC610.10886636207881212No Hit
TGATTGACACTGGGATTAGATACCCCCCCTAGAGGAGCCTGTTCTATTCC590.10529697315819532No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAT157.2037165E-6295.09
GATACTG255.2386895E-10295.06
ACATGGG157.2037165E-6295.07
TTACTGG108.4435777E-4295.04
TGATTGC255.2386895E-10295.01
TGACATG157.2037165E-6295.05
TGATTAC350.0295.01
GATTGAC54750.0295.02
CATGGGA157.2037165E-6295.08
GCACTGG255.2386895E-10295.06
GATTACT108.4435777E-4295.02
GATTACA255.2386895E-10295.02
ATACTGG255.2386895E-10295.07
ACTGGGT157.2037165E-6295.09
ACTGGAA108.4435777E-4295.09
TGATTGA55150.0294.732541
TGACACT54600.0294.729865
TTGACAC54700.0294.460724
GACACTG54650.0294.19036
ACACTGG55050.0293.392367
Produced by FastQC (version 0.11.5)