FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-173-6-1-1-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-173-6-1-1-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55653
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG2313641.57188291736295No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG34856.262016423193718No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG19973.588306111081164No Hit
GGTACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACCTAGCTGTC17063.0654232476236682No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC15542.792302301762708No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG11612.086140908845884No Hit
GGTACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG11322.034032307332938No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG11152.0034858857563833No Hit
GGTACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT10941.9657520708676979No Hit
GGTACACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATAT9921.7824735414083697No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG8631.5506801070921605No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG7481.3440425493684078No Hit
GGTACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG7211.2955276445115267No Hit
GGTACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT6961.250606436310711No Hit
GGTACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG5651.0152193053384364No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGATGC5611.008031912026306No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG5090.9145957989686091No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG5080.9127989506405764No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG3880.6971771512766608No Hit
GGTACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTACC3300.5929599482507681No Hit
GGTACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTACCTAGCTG3210.5767883132984745No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATTA2980.5354608017537239No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTACCCCTAAACTCTAATAG2190.39350978383914614No Hit
GGTACACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTACCTAG2170.3899160871830809No Hit
GGTACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT2140.384525542198983No Hit
GGTACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACCTAGCTGT1840.33062009235800405No Hit
GGTACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT1650.29647997412538407No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATAG1640.29468312579735145No Hit
GGTACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACCTAGC1590.2856988841571883No Hit
GGTACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTACCT1580.28390203582915563No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGATAC1510.27132409753292724No Hit
GGTACACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATAA1510.27132409753292724No Hit
GGTACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTACCTAGCT1500.2695272492048946No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAGTAG1470.2641367042207967No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATAG1470.2641367042207967No Hit
GGTACACTGGGATTAGATACCCCTCTATACTAAATTACTTTATCCTATTA1320.23718397930030727No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATAG1280.22999658598817674No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACAG1200.21562179936391568No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTTGGTAA1180.21202810270785044No Hit
GGTACACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAATAG1170.21023125437981782No Hit
GGTACACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATAA1120.20124701273965465No Hit
GGTACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTAG1090.19585646775555676No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGACAG1030.18507537778736097No Hit
GGTACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT1000.17968483280326308No Hit
GGTACACTGGGATTAGATACCCCCCCTAGAGGAGCCTGTTCTACCTAGCT1000.17968483280326308No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCGTAAACCCAGACGT990.17788798447523044No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTACC990.17788798447523044No Hit
GGTACACTGGGATTAGATACCCCACGAGACAGAGGAGCCTGTTCTACCTA940.1689037428350673No Hit
GGTACACTGGGATTAGATACCCCGTCTCGTCTAGAGGAGCCTGTTCTACC920.16531004617900202No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAACAG920.16531004617900202No Hit
GGTACACTGGGATTAGATACCCCATTATTCTCTTTTTTAATTTTTTTTTA900.1617163495229368No Hit
GGTACACTGGGATTAGATACCCCCCCGTCCTAGAGGAGCCTGTTCTACCT890.15991950119490414No Hit
GGTACACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTACCTAGCTG890.15991950119490414No Hit
GGTACACTGGGATTAGATACCCCTGGCGAGAGACTGTCTCTAGAGGAGCC860.15452895621080623No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAACAG840.150935259554741No Hit
GGTACACTGGGATTAGATACCCCCTATGCTTAGCCCTAAACTCTAATAGT830.14913841122670835No Hit
GGTACACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTACCTAGCT830.14913841122670835No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGATGA810.1455447145706431No Hit
GGTACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACCTAGCT790.14195101791457782No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAC780.1401541695865452No Hit
GGTACATGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT730.13116992794638205No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAACAG710.12757623129031678No Hit
GGTACACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT630.11320144466605575No Hit
GGTACACTGGGATTAGATACCCCTGACGCTAAGAGGAGCCTGTTCTACCT600.10781089968195784No Hit
GGTACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT590.10601405135392522No Hit
GGTACACTGGGATTAGATACCCCTCGTCGGCAGCGTCAGATGTGTATAGA590.10601405135392522No Hit
GGTACACTGGGATTAGATACCCCGTCTCTCTAGAGGAGCCTGTTCTACCT580.10421720302589259No Hit
GGTACACTGGGATTGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT580.10421720302589259No Hit
GGTACACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTAC570.10242035469785996No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAGATAG570.10242035469785996No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGTT108.444514E-4294.987437
CACTGGG54500.0294.987435
CACTGGA550.0294.987435
TACACGG206.133814E-8294.987433
CTGGATT550.0294.987437
CGGGATT108.444514E-4294.987437
ACGGGAT108.444514E-4294.987436
GGGATTG108.444514E-4294.987439
GGGATAG108.444514E-4294.987439
TGGGTTA108.444514E-4294.987438
TGGGATA108.444514E-4294.987438
ACTGGGT108.444514E-4294.987436
ACTGGGG108.444514E-4294.987436
ACTGGAT550.0294.987436
ACACGGG157.2047023E-6294.98744
CATGGGA157.2047023E-6294.98745
ACACTGG55100.0294.719764
ACTGGGA54350.0294.716036
CTGGGAT54400.0294.445167
TACACTG55300.0294.18733
Produced by FastQC (version 0.11.5)