FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-172-6-7-4-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-172-6-7-4-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51131
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA2978158.244509201854065No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGA23124.521718722497115No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAA14012.740020731063347No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAA12252.395806849073947No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAA11582.2647708826348008No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGA11302.210009583227396No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAA9001.7601846238094305No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGA8301.6232813752909196No Hit
TCGGTCAAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAA8301.6232813752909196No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAA6101.1930140228041697No Hit
TCGGTCAAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCA5531.0815356632962392No Hit
TCGGTCAAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAA4430.8664019870528642No Hit
TCGGTCAAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAA4340.84880014081477No Hit
TCGGTCAAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAA3900.7627466703174199No Hit
TCGGTCAAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCC3710.7255872171481098No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAA3390.663002874968219No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGA2660.5202323443703428No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAGA2630.5143650622909781No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTCGA2490.4869844125872758No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGA2280.44591343803172245No Hit
TCGGTCAAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAA2200.43026735248674974No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAA2160.4224443097142634No Hit
TCGGTCAAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGACGTGG1730.338346599910035No Hit
TCGGTCAAACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAG1720.33639083921691343No Hit
TCGGTCAAACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAGCTCTAA1570.30705442882008954No Hit
TCGGTCAAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC1490.2914083432751169No Hit
TCGGTCAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC1470.2874968218888737No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCATAGCCCTAAACCCTGA1410.2757622577301441No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGTCTAGCCGTAAACCTTGA1390.27185073634390094No Hit
TCGGTCAAACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAA1360.2659834542645362No Hit
TCGGTCAAACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAA1330.2601161721851714No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATA1320.2581604114920498No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAGA1300.25424889010580665No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGA1290.25229312941268506No Hit
TCGGTCAAACTGGGATTAGATACCCAACTATGCTTAGCCCTAAACTATAA1130.22100095832273964No Hit
TCGGTCAAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAAC960.18775302653967263No Hit
TCGGTCAAACTGGGATTAGATACCCCTGTAGTCCACGCCCTAAACGATGA830.16232813752909195No Hit
TCGGTCAAACTGGGATTAGATACCCCGGTAGTCCACGCCCTAAACGCTGT830.16232813752909195No Hit
TCGGTAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC730.14277053059787603No Hit
TCGGTCAAACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGACG710.13885900921163286No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGA690.1349474878253897No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAA630.12321292366666015No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTTAGACCTAAACCTCAA630.12321292366666015No Hit
TCGGTCAAACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCC600.11734564158729537No Hit
TCGGTCAAACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC580.11343412020105219No Hit
TCGGTCAAATGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC550.10756683812168744No Hit
TCGGTCAAACTGGGATTAGATACCCCAGTAGTCTACGCCCTAAACGATGA540.10561107742856585No Hit
TCGGTCAAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAA520.10169955604232267No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTCA50850.0294.984341
TCGGTAA255.2386895E-10294.984341
GGTCAAC157.2015555E-6294.984343
ACGGGAT303.6379788E-12294.984349
CGGTCAA50850.0294.984342
ACTGGGT108.442758E-4294.984349
ACTGGAT206.129267E-8294.984349
AACGGGA303.6379788E-12294.984348
CAAACGG350.0294.984346
GTCAGCC108.442758E-4294.98434295
CGGTAAA206.129267E-8294.984342
GGTCAAA50750.0294.693733
AAACTGG50350.0293.812627
TCAAACT50350.0293.812625
CAAACTG50350.0293.812626
GTCAAAC50950.0292.3794
AACTGGG50350.0292.347968
ACTGGGA50200.0292.340069
TCAAACG400.0258.11135
AAACGGG351.2732926E-11252.843727
Produced by FastQC (version 0.11.5)