FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-171-6-2-6-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-171-6-2-6-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50518
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA2944758.29011441466408No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA21794.313314066273407No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA10922.1616057642820383No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA9741.9280256542222576No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA9341.8488459558969081No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATA9031.7874816896947623No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA8091.6014093986301912No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT7681.5202502078467082No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA7591.5024347757235044No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA7501.4846193436003008No Hit
GCCACAACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAATA6971.379706243319213No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA6101.207490399461578No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA5941.1758185201314382No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAG4590.9085870382833842No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA4020.7957559681697612No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT3680.7284532245932144No Hit
GCCACAACTGGGATTAGATACCCCCACTAGAGGAGCCTGTTCTAGACGTG3590.7106377924700107No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAACCCTAACA3350.6631299734748011No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA3290.6512530187259987No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAACA3160.6255196167702601No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCCAAACCTCAACA3070.6077041846470564No Hit
GCCACAACTGGGATTAGATACCCCTACGACTCACTAGAGGAGCCTGTTCT2690.5324834712379746No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAACA2640.5225860089473059No Hit
GCCACAACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGACGT2500.49487311453343363No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA2310.45726275782889264No Hit
GCCACAACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGACGTG2150.4255908784987529No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGATA2090.41371392374995053No Hit
GCCACAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG2020.39985747654301435No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA1950.3860010293360782No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC1730.34245219525713605No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGACGTGGCT1560.3088008234688626No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA1330.26327249693178667No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAG1320.26129300447365295No Hit
GCCACAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT1260.24941604972485054No Hit
GCCACAACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGACGT1190.2355596025179144No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGACGTG1190.2355596025179144No Hit
GCCACAACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATA1180.2335801100597807No Hit
GCCACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1160.2296211251435132No Hit
GCCACAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1100.2177441703947108No Hit
GCCACAACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGACGT960.19003127598083852No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACA710.14054396452749515No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACACGACTTGATCTC690.13658497961122767No Hit
GCCACAACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG620.12272853240429153No Hit
GCCACAACTGGGATTGATACCCCACTATGCTTAGCCCTAAACCTCAACAG610.12074903994615781No Hit
GCCACAACTGGGATTAATACCCCACTATGCTTAGCCCTAAACCTCAACAG560.11085157765548914No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACA540.10689259273922166No Hit
GCCACAATGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG510.10095411536482048No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTGGG49650.0294.993076
AACTGGA450.0294.993076
CAACTGG50100.0294.993075
CACTGGG157.200204E-6294.993075
CTGGATT303.6379788E-12294.993078
CGGGATT108.4417174E-4294.993078
ACGGGAT108.4417174E-4294.993077
TGGGATA303.6379788E-12294.993079
AACGGGA108.4417174E-4294.993076
CAACGGG108.4417174E-4294.993075
CTGGGAT49750.0294.103648
ACTGGGA49750.0294.103647
TGGGATT49500.0294.099159
GCCACAA50550.0293.825931
CACAACT52000.0284.498143
CCACAAC52150.0284.245482
ACAACTG52400.0282.044924
ACTGGAT450.0262.216067
TGGATTA351.2732926E-11252.85129
GGAACAA150.0028418698196.66205295
Produced by FastQC (version 0.11.5)