FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-170-6-1-1-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-170-6-1-1-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49308
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG2204644.71079743652145No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG24865.041778210432384No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG14903.021822016711284No Hit
GGTACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAACTTCGCCTG14012.841323923095644No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC13292.6953029934290584No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG11902.4134014764338447No Hit
GGTACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG10282.084854384684027No Hit
GGTACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT10052.038208809929423No Hit
GGTACACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATAT9141.8536545793785997No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG7331.4865741867445446No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG7101.4399286119899406No Hit
GGTACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT6641.3466374624807333No Hit
GGTACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG5971.2107568751521052No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG5801.17627971120305No Hit
GGTACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG5091.032286850004056No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG4370.8862659203374706No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG3670.7443011276060679No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGATGC3670.7443011276060679No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG2950.5982801979394824No Hit
GGTACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAAC2870.5820556501987507No Hit
GGTACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAACTTCGCC2680.5435223493145128No Hit
GGTACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAACT2530.5131013223006409No Hit
GGTACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT2400.48673643222195184No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATTA1980.40155755658311026No Hit
GGTACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAACTTCGCCT1880.3812768719071956No Hit
GGTACACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTAACTTCGC1710.34679970795814064No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATAG1640.33260322868500036No Hit
GGTACACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTAACTTC1640.33260322868500036No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTACCCCTAAACTCTAATAG1590.32246288634704307No Hit
GGTACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTAG1550.3143506124766772No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGATAC1470.2981260647359455No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATAG1390.28190151699521376No Hit
GGTACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT1340.2717611746572564No Hit
GGTACACTGGGATTAGATACCCCACGAGACAGAGGAGCCTGTTCTAACTT1320.2677050377220735No Hit
GGTACACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATAA1230.2494524215137503No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAACAG1180.239312079175793No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACAG1120.2271436683702442No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAGTAG1080.21903139449987835No Hit
GGTACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAACTTCG1070.21700332603228684No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAGATAG1070.21700332603228684No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATAG1030.208891052161921No Hit
GGTACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT970.19672264135637219No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGACAG950.19266650442118927No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCGTAAACCCAGACGT940.1906384359535978No Hit
GGTACACTGGGATTAGATACCCCGCACTAGAGGAGCCTGTTCTAACTTCG940.1906384359535978No Hit
GGTACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAACTTCGC910.1845542305508234No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTTGGTAA900.1825261620832319No Hit
GGTACACTGGGATTAGATACCCCATTATTCTCTTTTTTAATTTTTTTTTA870.17644195668045753No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATCGATTG830.16832968281009167No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAAC790.1602174089397258No Hit
GGTACACTGGGATTAGATACCCCTCTATACTAAATTACTTTATCCTATTA750.15210513506935994No Hit
GGTACACTGGGATTAGATACCCCAATACGCTTAGCCCTAAACTCCAATAG750.15210513506935994No Hit
GGTACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAACTTCGC730.14804899813417702No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACGG700.1419647927314026No Hit
GGTACACTGGGATTAGATACCCCCTATGCTTAGCCCTAAACTCTAATAGT680.13790865579621966No Hit
GGTACACTGGGATTAGATACCCCCCCTAGAGGAGCCTGTTCTAACTTCGC680.13790865579621966No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG680.13790865579621966No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAACAG620.12574024499067088No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGATGA620.12574024499067088No Hit
GGTACACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATAA610.12371217652307942No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACAG600.12168410805548796No Hit
GGTACACTGGGATTAGATACCCCTGACGCTAAGAGGAGCCTGTTCTAACT600.12168410805548796No Hit
GGTACACTGGGATTAGATACCCCGTCTCTCTAGAGGAGCCTGTTCTAACT600.12168410805548796No Hit
GGTACACTGGGATTAGATACCCCACGAGACGTCTAGAGGAGCCTGTTCTA600.12168410805548796No Hit
GGTACACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT560.1135718341851221No Hit
GGTACACTGGGATTAGATACCCCACGAGAGGAGCCTGTTCTAACTTCGCC510.10343149184716477No Hit
GGTACACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCCT510.10343149184716477No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAA510.10343149184716477No Hit
GGTACACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAACTTCGC500.1014034233795733No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGGG157.1997747E-6294.986824
TAGGATT157.1997747E-6294.986828
CACTGGG48350.0294.986825
CTGGATT303.6379788E-12294.986827
TGGATTA303.6379788E-12294.986828
GGGATAG350.0294.986829
ACTGGAT303.6379788E-12294.986826
GGTACAC49150.0294.986821
GTACACT48950.0294.685522
ACTGGGA48400.0294.68216
CTGGGAT48450.0294.377967
TACACTG48850.0294.081023
ACACTGG48800.0294.08014
TGGGATT48100.0294.06698
GGGATTA48150.0293.455239
TGGGATA400.0258.113468
GTACACG251.8645005E-7235.989472
AGGATTA202.2693486E-5221.240139
CACTGGA403.274181E-11221.240135
TACACGG202.2693486E-5221.240133
Produced by FastQC (version 0.11.5)