FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-17-6-4-8-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-17-6-4-8-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11554
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAA529045.785009520512375No Hit
TGATTGACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCA10238.854076510299464No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGA3653.1590791068028388No Hit
TGATTGACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAATAGGAT3543.0638739830361783No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAA3362.908083780508915No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGA3362.908083780508915No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGA3222.78691362298771No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAA3152.7263285442271075No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTTA2782.406093127921066No Hit
TGATTGACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCT2682.31954301540592No Hit
TGATTGACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAA2602.250302925393803No Hit
TGATTGACACTGGGATTAGATACCCCTCTATACTAAATTACTTTATCCTA2322.0079626103513935No Hit
TGATTGACACTGGGATTAGATACCCCTGGCGAGAGACTGTCTCTAGAGGA1921.6617621602908084No Hit
TGATTGACACTGGGATTAGATACCCCTCGTCGGCAGCGTCAGATGTGTAT1761.5232819802665742No Hit
TGATTGACACTGGGATTAGATACCCCTCCGAGCCCACGAGACTAGAGGAG1461.2636316427211354No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAA1281.107841440193872No Hit
TGATTGACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCC1211.0472563614332697No Hit
TGATTGACACTGGGATTAGATACCCCAGTAGTCCTAGAGGAGCCTGTTCT1181.021291327678726No Hit
TGATTGACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAA980.8481911026484333No Hit
TGATTGACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAA920.7962610351393457No Hit
TGATTGACACTGGGATTAGATACCCCTGCTAGAGGAGCCTGTTCTAATAG910.7876060238878311No Hit
TGATTGACACTGGGATTAGATACCCCATTAATACGACTCACTAGAGGAGC810.7010559113726847No Hit
TGATTGACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC300.2596503375454388No Hit
TGATTGACACTGGGATTAGATACCCCCCCTAGAGGAGCCTGTTCTAATAG250.21637528128786568No Hit
TGATTGACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAA240.20772027003635105No Hit
TGATTGACACTGGGATTAGATACCCCTGACGCTGCTAGAGGAGCCTGTTC210.18175523628180718No Hit
TGATTGACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAATAG190.1644452137787779No Hit
TGATTGAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC130.11251514626969014No Hit
TGATTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC130.11251514626969014No Hit
TGATTGACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAAC120.10386013501817552No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACACT11350.0295.05
CACTGGA108.3570444E-4295.08
GACACTG11250.0295.06
GATTGAC11500.0295.02
TTGACAC11350.0295.04
ACTGGAT108.3570444E-4295.09
ACACTGG11300.0293.694677
CACTGGG11200.0293.683048
ACTGGGA11200.0293.683049
TGATTGA11600.0292.45691
ATTGACA11550.0292.44593
GCCCTAA7400.059.00000435-39
TTCATAT6650.059.000004120-124
TATGCTT7100.059.00000425-29
AATCACA750.004271697359.000004295
GGATTAG11450.059.00000410-14
GAGCCAT452.5465852E-1159.00000485-89
CTTAGCC6950.059.00000430-34
CTGGCGG6700.059.000004110-114
CCACTAG900.059.00000425-29
Produced by FastQC (version 0.11.5)