FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-169-6-5-7-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-169-6-5-7-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48475
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAAC1622733.47498710675606No Hit
CTGGATGACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACCACTCCT28345.846312532233109No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGAT24285.008767405879319No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGAT23564.8602372356884995No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTA15213.137699845281073No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCAT14973.0881897885507996No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGAT14603.011861784424961No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAAT8291.7101598762248582No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGAT7591.5657555440948945No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGAT7471.5410005157297577No Hit
CTGGATGACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAAT6821.4069107787519342No Hit
CTGGATGACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAAT5641.1634863331614236No Hit
CTGGATGACTGGGATTAGATACCCCTCTATGCTTAGCCCTACACCCTAAC5541.1428571428571428No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAAC4891.0087674058793192No Hit
CTGGATGACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAC4851.000515729757607No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAT4690.9675090252707581No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAAT4590.9468798349664775No Hit
CTGGATGACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTACCACTC4230.8726147498710676No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAACCCTTGAT4060.8375451263537905No Hit
CTGGATGACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAAC4000.8251676121712224No Hit
CTGGATGACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGT3930.8107271789582258No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAAC3860.7962867457452295No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGAT3840.7921609076843734No Hit
CTGGATGACTGGGATTAGATACCCCCCCTCTAGAGGAGCCTGTTCTACCA3670.7570912841670965No Hit
CTGGATGACTGGGATTAGATACCCCCCCCTAGAGGAGCCTGTTCTACCAC3550.7323362558019597No Hit
CTGGATGACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACCACT3460.7137699845281072No Hit
CTGGATGACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCA2520.51985559566787No Hit
CTGGATGACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACCACT2450.5054151624548736No Hit
CTGGATGACTGGGATTAGATACCCCCTGGAGCTAGAGGAGCCTGTTCTAC2450.5054151624548736No Hit
CTGGATGACTGGGATTAGATACCCCCTCTCTCCCTCTCCTAGAGGAGCCT2380.49097472924187724No Hit
CTGGATGACTGGGATTAGATACCCCTCCCTAGAGGAGCCTGTTCTACCAC2300.47447137699845277No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAAC2300.47447137699845277No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGAT2240.4620938628158845No Hit
CTGGATGACTGGGATTAGATACCCTGGGAATGGAGGAGCCTGTTCTACCA2230.46003094378545645No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTATAAT2200.45384218669417226No Hit
CTGGATGACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTACCACT2190.4517792676637442No Hit
CTGGATGACTGGGATTAGATACCCCAACTAGAGGAGCCTGTTCTACCACT2140.4414646725116039No Hit
CTGGATGACTGGGATTAGATACCCCCCCCTCTAGAGGAGCCTGTTCTACC2100.4332129963898917No Hit
CTGGATGACTGGGATTAGATACCCCTGAGAGCTAGAGGAGCCTGTTCTAC2040.42083548220732336No Hit
CTGGATGACTGGGATTAGATACCCCCCGGGGCTAGAGGAGCCTGTTCTAC2000.4125838060856112No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATAGAT1940.4002062919030428No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTGGCCGTAAACTTTGAT1850.3816400206291903No Hit
CTGGATGACTGGGATTAGATACCCCGACTAGAGGAGCCTGTTCTACCACT1740.35894791129448167No Hit
CTGGATGACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAG1670.3445074780814853No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAAT1500.30943785456420836No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAAT1460.3011861784424961No Hit
CTGGATGACTGGGATTAGATACCCCCCGGATGTGTAGAGGAGCCTGTTCT1350.2784940691077875No Hit
CTGGATGACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCT1260.259927797833935No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTCAGCCCTAATCTTCAAC1230.25373904074265086No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAAC1160.23929860752965448No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTAAT1130.2331098504383703No Hit
CTGGATGACTGGGATTAGATACCCTAGAGGAGCCTGTTCTACCACTCCTG1090.22485817431665808No Hit
CTGGATGACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA1000.2062919030428056No Hit
CTGGATGACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAAC930.19185146982980916No Hit
CTGGATGACTGGGATTAGATACCCCCAGGAGCCTGTTCTACCACTCCTGT880.1815368746776689No Hit
CTGGATGACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTAC740.1526560082516761No Hit
CTGGATGACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAGT700.1444043321299639No Hit
CTGGATGACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACA670.13821557503867973No Hit
CTGGATGACTGGGATTAGATACCCCTACTACAGGCGACCGTTCACATGGG660.13615265600825166No Hit
CTGGATGACTGGGATTAGATACCCTCGTTGGCAGCGTCAGATGTGTATAA660.13615265600825166No Hit
CTGGATGACTGGGATTAGATACCCCCACCCTAGAGGAGCCTGTTCTACCA580.11964930376482724No Hit
CTGGATACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA540.11139762764311502No Hit
CTGGATGACTGGGATTAGATACCCCACCAGGCACTCGCAAGGCGACCCAG490.10108303249097472No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGCT108.441304E-4294.985573
GGATGAT157.199131E-6294.985573
GGATGAC47800.0294.985573
GCTGGGA108.441304E-4294.985577
GACGGGA157.199131E-6294.985577
CTGGATG48100.0294.985571
CGGGATT157.199131E-6294.985579
TACTGGG157.199131E-6294.985576
ACGGGAT157.199131E-6294.985578
TGGATGC108.441304E-4294.985572
CTGGATT255.2386895E-10294.985539
TGGATGA48000.0294.678282
TGACTGG47450.0294.67476
GACTGGG47200.0294.36067
GATGACT47850.0293.75264
ATGACTG47700.0293.748725
ACTGGGA47400.0293.118568
CTGGGAT47550.0292.504069
ATGACGG202.2691467E-5221.239175
GGATACT202.2691467E-5221.239173
Produced by FastQC (version 0.11.5)