FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-168-6-2-6-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-168-6-2-6-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48120
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA2723956.60640066500415No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA19153.9796342477140487No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT18363.815461346633416No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA12332.562344139650873No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGCTGAGAAC11202.3275145469659186No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA10832.2506234413965087No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA9551.984621778886118No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA8401.7456359102244388No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA6831.4193682460515378No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA5651.1741479634247713No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA5601.1637572734829593No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATA5261.0931005818786368No Hit
GCCACAACTGGGATTAGATACCCCTGTAGTCCCTAGAGGAGCCTGTTCTA4380.9102244389027431No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA2900.6026600166251039No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTGAACTTCAACA2870.5964256026600165No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTAACA2840.5901911886949294No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA2640.5486284289276808No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTCGATA2570.5340814630091438No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAACA2530.525768911055694No Hit
GCCACAACTGGGATTAGATACCCCACCGTGCTTAGCCCTAAACCTCAACA2450.5091438071487947No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT2110.4384871155444722No Hit
GCCACAACTGGGATTAGATACCCCCGAGCTAGAGGAGCCTGTTCTAGCTG2080.43225270157938483No Hit
GCCACAACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTAGCTGA2050.4260182876142976No Hit
GCCACAACTGGGATTAGATACCCCCGACCTAGAGGAGCCTGTTCTAGCTG1800.3740648379052369No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCGTAAACCCAGACG1760.3657522859517872No Hit
GCCACAACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC1680.3491271820448878No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGATA1530.3179551122194514No Hit
GCCACAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1490.3096425602660017No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAG1430.2971737323358271No Hit
GCCACAACTGGGATTAGATACCCCGCTGTTGGAGACTAGAGGAGCCTGTT1430.2971737323358271No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC1370.28470490440565255No Hit
GCCACAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT1240.257689110556941No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA1100.228595178719867No Hit
GCCACAACTGGGATTAGATACCCCCCACCTAGAGGAGCCTGTTCTAGCTG1080.22443890274314215No Hit
GCCACAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1060.22028262676641727No Hit
GCCACAACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTAGCTGA1020.2119700748129676No Hit
GCCACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1010.20989193682460516No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCGTAAACCTTGATA1000.20781379883624274No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA990.2057356608478803No Hit
GCCACAACTGGGATTAGATACCCCTCTCCTAGAGGAGCCTGTTCTAGCTG910.18911055694098086No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG900.18703241895261846No Hit
GCCACAACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTAGCTGAG850.17664172901080633No Hit
GCCACAACTGGGATTAGATACCCCCGAGCCCTAGAGGAGCCTGTTCTAGC850.17664172901080633No Hit
GCCACAACTGGGATTAGATACCCCGGGTAGTCTAGAGGAGCCTGTTCTAG670.13923524522028263No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACA660.1371571072319202No Hit
GCCACAACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG620.1288445552784705No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTATAATA620.1288445552784705No Hit
GCCACAACTGGGATTAGATACCCAACTATGCTTAGCCCTAAACTATAATA610.12676641729010807No Hit
GCCACAACTGGGATTAGATACCCCACTATGATTAGCCCTAAACTTCAACA570.11845386533665835No Hit
GCCACAACTGGGATTGATACCCCACTATGCTTAGCCCTAAACCTCAACAG550.1142975893599335No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACA550.1142975893599335No Hit
GCCACAATGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG530.11014131338320864No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAACA520.10806317539484621No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAT108.439877E-4295.07
CAATGGG108.439877E-4295.05
AACTGGG47150.0295.06
ACACTGG157.197401E-6295.04
CTGGATT157.197401E-6295.08
CGGGATT206.12381E-8295.08
TGGATTA157.197401E-6295.09
AATGGGA108.439877E-4295.06
ACGGGAT206.12381E-8295.07
TGGGATA108.439877E-4295.09
ACTGGAT157.197401E-6295.07
AACGGGA206.12381E-8295.06
ACAATGG108.439877E-4295.04
CAACTGG47400.0294.68885
GCCACAA48000.0294.078121
CTGGGAT47300.0293.752628
ACTGGGA47300.0293.752627
TGGGATT47300.0292.81719
CCACAAC49050.0286.580022
CACAACT49250.0283.91883
Produced by FastQC (version 0.11.5)