FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-167-6-8-1-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-167-6-8-1-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47875
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG2145244.80835509138381No Hit
GGTACACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTAGATTGAGACT21714.534725848563969No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATAG21284.444908616187989No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATAG14773.085117493472585No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACAG11732.450130548302872No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATAC9441.9718015665796345No Hit
GGTACACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATTT9161.9133159268929505No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATAG8141.700261096605744No Hit
GGTACACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATAT7741.616710182767624No Hit
GGTACACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACAG6391.3347258485639688No Hit
GGTACACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAAT5711.1926892950391645No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATAG5541.1571801566579636No Hit
GGTACACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATAG5391.1258485639686684No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATAG5191.0840731070496084No Hit
GGTACACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATAG4550.9503916449086162No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACAG4500.9399477806788512No Hit
GGTACACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTAGATTGAGAC3710.7749347258485639No Hit
GGTACACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTAGATTGAGA3510.7331592689295039No Hit
GGTACACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAGA2950.6161879895561359No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATAG2910.6078328981723238No Hit
GGTACACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTAGAT2810.5869451697127938No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGATGC2710.5660574412532637No Hit
GGTACACTGGGATTAGATACCCCGTCTCTAGAGGAGCCTGTTCTAGATTG2320.4845953002610966No Hit
GGTACACTGGGATTAGATACCCCCCCGTCCTAGAGGAGCCTGTTCTAGAT1950.4073107049608355No Hit
GGTACACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTAGATTGA1870.39060052219321145No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATAG1850.38642297650130547No Hit
GGTACACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTAGATTGAG1850.38642297650130547No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTCAACAG1840.3843342036553525No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTACCCCTAAACTCTAATAG1840.3843342036553525No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCCAACAG1750.36553524804177545No Hit
GGTACACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGTT1510.3154046997389034No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATAG1510.3154046997389034No Hit
GGTACACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCAAATAG1460.3049608355091384No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATAG1460.3049608355091384No Hit
GGTACACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTAGATTGAG1370.28616187989556136No Hit
GGTACACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAACTCTAGTAG1270.26527415143603134No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATTA1240.25900783289817236No Hit
GGTACACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT1230.2569190600522193No Hit
GGTACACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTAGATTGAG1220.2548302872062663No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAACCTTGGTAA1070.2234986945169713No Hit
GGTACACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATAA1010.21096605744125324No Hit
GGTACACTGGGATTAGATACCCCACGAGACAGAGGAGCCTGTTCTAGATT1000.20887728459530025No Hit
GGTACACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATAA1000.20887728459530025No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCTCAACAG980.20469973890339427No Hit
GGTACACTGGGATTAGATACCCCATTATTCTCTTTTTTAATTTTTTTTTA960.20052219321148823No Hit
GGTACACTGGGATTAGATACCCCTGTAGTCCTAGAGGAGCCTGTTCTAGA950.19843342036553524No Hit
GGTACACTGGGATTAGATACCCCCCCTAGAGGAGCCTGTTCTAGATTGAG910.19007832898172322No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCGACAG900.18798955613577023No Hit
GGTACACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT900.18798955613577023No Hit
GGTACACTGGGATTAGATACCCCTCGTCGGCAGCGTCAGATGTGTATAGA880.18381201044386425No Hit
GGTACACTGGGATTAGATACCCCACGAGAGGAGCCTGTTCTAGATTGAGA810.1691906005221932No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAGTAG800.1671018276762402No Hit
GGTACACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGATAC780.1629242819843342No Hit
GGTACACTGGGATTAGATACCCCGCACTAGAGGAGCCTGTTCTAGATTGA780.1629242819843342No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTGAACCTCAACAG740.15456919060052218No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCTTAAACATAGACAG700.14621409921671016No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAGATAG650.13577023498694518No Hit
GGTACACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTAG640.13368146214099216No Hit
GGTACACGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT600.12532637075718017No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACAG600.12532637075718017No Hit
GGTACACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTGT570.11906005221932114No Hit
GGTACACTGGGATTAGATACCCCTCCGAGCCCACGAGACCTAGAGGAGCC540.11279373368146214No Hit
GGTACACTGGGATTAGATACCCCTCCGAGCCCACGAGACTAGAGGAGCCT530.11070496083550915No Hit
GGTACATGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGT530.11070496083550915No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACGG510.10652741514360314No Hit
GGTACACTGGGATTAGATACCCCACCATGCTTAGCCCTAAACCTCAACAG510.10652741514360314No Hit
GGTACACTGGGATTAGATACCCCTTCTAGAGGAGCCTGTTCTAGATTGAG500.10443864229765012No Hit
GGTACACTGGGATTAGATACCCCCCTATGCTTAGCCCTAAACTCTAATAG490.10234986945169713No Hit
GGTACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACATCGATTG490.10234986945169713No Hit
GGTACACTGGGATTAGATACCCCACTATGCTCAGCCGTAAACCCAGACGT480.10026109660574412No Hit
GGTACACTGGGATTAGATACCCCTCTATACTAAATTACTTTATCCTATTA480.10026109660574412No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTGGG108.442783E-4294.964485
ATGGGAT206.127266E-8294.964486
ACACGGG255.2386895E-10294.964484
GGGTTAG108.442783E-4294.964489
CTGGGTT108.442783E-4294.964487
TCTGGGA108.442783E-4294.964486
ACTCTGG108.442783E-4294.964484
CACTGGG46800.0294.964485
TACACGG255.2386895E-10294.964483
CGGGATT255.2386895E-10294.964487
CTGGAAT108.442783E-4294.964487
ACGGGAT255.2386895E-10294.964486
TGGAATT108.442783E-4294.964488
ACTGGGT108.442783E-4294.964486
GGTACTC108.442783E-4294.964481
GGTACAT206.127266E-8294.964481
GGTACAC47450.0294.964481
CACGGGA255.2386895E-10294.964485
CTGGGAT46900.0294.335547
ACTGGGA46800.0294.33426
Produced by FastQC (version 0.11.5)