FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-166-6-2-6-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-166-6-2-6-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46539
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA2033943.703130707578595No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA33147.120909344850555No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT21594.639119877951825No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA16023.442274221620576No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA14073.02327080513118No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTACCACTCCTG13552.911536560734008No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA11792.5333591181589634No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA11742.5226154408130816No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA6291.351554610111949No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA6221.3365134618277144No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA6021.2935387524441866No Hit
GCCACAACTGGGATTAGATACCCCACTATGTTTAGCCATAAACTTAAAGT5941.2763488686907754No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA5091.0937063538107825No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA4300.9239562517458475No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA4030.865940394078085No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA3730.8014783300027934No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAGATA3720.799329594533617No Hit
GCCACAACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTACCACTC2940.6317282279378585No Hit
GCCACAACTGGGATTAGATACCCCCACGAGACCTAGAGGAGCCTGTTCTA2890.6209845505919767No Hit
GCCACAACTGGGATTAGATACCCCCCTAGAGGAGCCTGTTCTACCACTCC2810.6037946668385654No Hit
GCCACAACTGGGATTAGATACCCCGGTAGTCCCTAGAGGAGCCTGTTCTA2730.5866047830851544No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAAGTA2620.562968692924214No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCATAAACTTAAATA2340.5028040997872751No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA2150.46197812587292375No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATA1870.40181353273598486No Hit
GCCACAACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTACCACTCCT1780.38247491351339735No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAC1540.330905262253164No Hit
GCCACAACTGGGATTAGATACCCCCAACCTAGAGGAGCCTGTTCTACCAC1530.32875652678398765No Hit
GCCACAACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC1510.32445905584563484No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTTATG1450.3115666430305765No Hit
GCCACAACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTACCACT1390.29867423021551814No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTACCACTC1380.29652549474634177No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA1360.292228023807989No Hit
GCCACAACTGGGATTAGATACCCCTGACCGACGACTAGAGGAGCCTGTTC1350.29007928833881264No Hit
GCCACAACTGGGATTAGATACCCCTCGTCGATGCTAGAGGAGCCTGTTCT1300.27933561099293064No Hit
GCCACAACTGGGATTAGATACCCCCCCCTCTAGAGGAGCCTGTTCTACCA1250.2685919336470487No Hit
GCCACAACTGGGATTAGATACCCCCCTCTAGAGGAGCCTGTTCTACCACT1210.2599969917703432No Hit
GCCACAACTGGGATTAGATACCCCCCCTAGAGGAGCCTGTTCTACCACTC1210.2599969917703432No Hit
GCCACAACTGGGATTAGATACCCCAGTAGTCCCTAGAGGAGCCTGTTCTA1190.2556995208319904No Hit
GCCACAACTGGGATTAGATACCCCTGTAGAGGAGCCTGTTCTACCACTCC1140.24495584348610844No Hit
GCCACAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1120.24065837254775563No Hit
GCCACAACTGGGATTAGATACCCCTGACGCCTAGAGGAGCCTGTTCTACC1090.2342121661402265No Hit
GCCACAACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTACCACTCC930.19983239863340424No Hit
GCCACAACTGGGATTAGATACCCCCACCTAGAGGAGCCTGTTCTACCACT930.19983239863340424No Hit
GCCACAACTGGGATTAGATACCCCGTCTCGCTAGAGGAGCCTGTTCTACC880.18908872128752227No Hit
GCCACAACTGGGATTAGATACCCCGAGAGAGAGAGAGAGAGGAGCCTGTT850.1826425148799931No Hit
GCCACAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG800.17189883753411117No Hit
GCCACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG750.16115516018822923No Hit
GCCACAACTGGGATTAGATACCCCGAGACCTAGAGGAGCCTGTTCTACCA750.16115516018822923No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTGGCCCTAAACCCTGATA730.15685768924987645No Hit
GCCACAACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTACCACT700.1504114828423473No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTGTCTCTTATACACATCTC700.1504114828423473No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACTCTCCGATATCTC680.1461140119039945No Hit
GCCACAACTGGGATTAGATACCCCCAGACCTAGAGGAGCCTGTTCTACCA670.1439652764348181No Hit
GCCACAACTGGGATTAGATACCCCACTATAGTTACATTAACAAAACCATT610.13107286361975978No Hit
GCCACAACTGGGATTAGATACCCCCCTCCTAGAGGAGCCTGTTCTACCAC600.12892412815058338No Hit
GCCACAACTGGGATTAGATACCCGGGTAGTCCTAGAGGAGCCTGTTCTAC560.12032918627387781No Hit
GCCACAACTGGGATTAGATACCCCAGTATGCCTGGCCCTAAATCTTGATA540.11603171533552505No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAGCCCTAAACCTCAACA510.10958550892799589No Hit
GCCACAACTGGGATTAGATACCCCGAGAGAGAGAGAGAGGAGCCTGTTCT490.10528803798964309No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGGG108.439618E-4294.99255
AATGGGA108.439618E-4294.99256
ACTGGGT108.439618E-4294.99257
CCTAGAA108.439618E-4294.9925295
ACAATGG108.439618E-4294.99254
AACTGGG45350.0294.992466
ACACTGG157.1965933E-6294.992464
CTGGATT303.6379788E-12294.992468
TGGATTA303.6379788E-12294.992469
TGGGATA157.1965933E-6294.992469
CAACTGG45800.0294.67045
ACTGGGA45500.0294.027
CTGGGAT45450.0294.018928
GCCACAA46450.0293.087251
TGGGATT45500.0293.04759
CCACAAC47500.0285.676942
CACAACT47750.0283.872343
ACAACTG48350.0280.044564
AACTGGA450.0262.21556
ACTGGAT400.0258.118447
Produced by FastQC (version 0.11.5)