FastQCFastQC Report
Sat 9 Mar 2019
000000000-BMH6J_l01_n01_lib-165-6-4-6-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BMH6J_l01_n01_lib-165-6-4-6-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46249
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACA2065144.65177625462172No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACCTTGATA25925.604445501524357No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGTTACATTAACAAAACCATT13372.890873316179809No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTGATA12852.778438452723302No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTGGCCCTAAATCTTGATA11922.5773530238491644No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCCCGCCGTAAACTTAGATA10582.287617029557396No Hit
GCCACAACTGGGATTAGATACCCCACGAGACCTAGAGGAGCCTGTTCTAT8401.816255486605116No Hit
GCCACAACTGGGATTAGATACCCCACTATGCACAGCCGTAAACTTTGATA8301.7946333974788644No Hit
GCCACAACTGGGATTAGATACCCCGTCTAGAGGAGCCTGTTCTATAAGCA8051.7405781746632358No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTAGATA7791.6843607429349823No Hit
GCCACAACTGGGATTAGATACCCCTAGAGGAGCCTGTTCTATAAGCACTG6201.3405695258275854No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTTCAACA5821.2584055871478304No Hit
GCCACAACTGGGATTAGATACCCCACTAGGCTTAGCCCTAAACTCCAATA5511.191377110856451No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACTTTTATG5221.128673052390322No Hit
GCCACAACTGGGATTAGATACCCCACTACACTTAGCCCTAAAGTCGAATA4580.9902916819823131No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCTAATA4190.9059655343899328No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCCTAAATCTCGATG3940.8519103115743044No Hit
GCCACAACTGGGATTAGATACCCCCCAATACGCTTAGCCCTAAACTCCAA3770.8151527600596769No Hit
GCCACAACTGGGATTAGATACCCCTCTATGCTTAGCCCTAAACCCTAACA3710.8021795065839261No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACACAAATA3110.6724469718264179No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGTTCTAAACCCAAATA3100.6702847629137927No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACCTCAACA2970.6421760470496659No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACTTCAACA2900.62704058466129No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACTCCAATA2770.5989318687971632No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTATATT2590.5600121083699107No Hit
GCCACAACTGGGATTAGATACCCCACGAGACTAGAGGAGCCTGTTCTATA2510.5427144370689095No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTACACAAATAGTTAAATT2480.5362278103310342No Hit
GCCACAACTGGGATTAGATACCCCACTGTGCTTAGCCCTAAGCTCTAATA2150.46487491621440463No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCTAATA2090.45190166273865384No Hit
GCCACAACTGGGATTAGATACCCCATACGTGCAGGGAGGGTACCTTGAGG2090.45190166273865384No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAGCCCTAAACCTCAACA1940.41946852904927673No Hit
GCCACAACTGGGATTAGATACCCCACTCTGCTTAGCCATACGCTCAAATA1930.4173063201366516No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACCCTAAACTCGAATA1850.4000086488356505No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCATAAACATTGACA1840.3978464399230254No Hit
GCCACAACTGGGATTAGATACCCCCTCTAGAGGAGCCTGTTCTATAAGCA1820.39352202209777504No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCTGATA1710.3697377240588986No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAACCTTAAACAACGATG1540.3329801725442712No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAGCA1460.31568250124327013No Hit
GCCACAACTGGGATTAGATACCCCGAGCCCACGAGACCTAGAGGAGCCTG1380.298384829942269No Hit
GCCACAACTGGGATTAGATACCCCCTAGAGGAGCCTGTTCTATAAGCACT1220.26378948734026686No Hit
GCCACAACTGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG1160.250816233864516No Hit
GCCACAACTGGGATTAGATACCCCGGTATTGATTTAGATTACCCAGATGC1070.23135635365088975No Hit
GCCACAACTGGGATTAGATACCCCGCTATGCTTAACTCTAAACTCGAATA1050.2270319358256395No Hit
GCCACAACTGGGATTAGATACCCCTCTAGAGGAGCCTGTTCTATAAGCAC990.21405868234988865No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCCTAAACATTGGTA990.21405868234988865No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTCAGCCGTAAACCCAGACG970.20973426452463836No Hit
GCCACAACTGGGATTAGATACCCCACTAGAGGAGCCTGTTCTATAAGCAC950.20540984669938808No Hit
GCCACAACTGGGATTAGATACCCCATCTAGAGGAGCCTGTTCTATAAGCA930.20108542887413783No Hit
GCCACAACTGGGATTAGATACCCCACCTAGAGGAGCCTGTTCTATAAGCA910.19676101104888752No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCGTAAACATTGATT890.19243659322363726No Hit
GCCACAACTGGGATTAGATACCCCGACCTAGAGGAGCCTGTTCTATAAGC860.18594996648576184No Hit
GCCACAACTGGGATAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG830.17946333974788645No Hit
GCCACAACTGGGATTAGATACCCCACTATGCCTAGCCGTAAACATCGATA760.1643278773595105No Hit
GCCACACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAG740.1600034595342602No Hit
GCCACAACTGGGATTAGATACCCCGCTAGAGGAGCCTGTTCTATAAGCAC740.1600034595342602No Hit
GCCACAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCCAGATA720.15567904170900992No Hit
GCCACAACTGGGATTAGATACCCCGTCTCGTCGTCGTCGTCGTCTAGAGG720.15567904170900992No Hit
GCCACAACTGGGATTAGATACCCCCGCCTAGAGGAGCCTGTTCTATAAGC710.15351683279638478No Hit
GCCACAACTGGGATTAGATACCCCGAGCCACTAGAGGAGCCTGTTCTATA710.15351683279638478No Hit
GCCACAACTGGGATTAGATACCCCAACCTAGAGGAGCCTGTTCTATAAGC650.14054357932063397No Hit
GCCACAACTGGGATTAGATACCCCAATACGCTTAGCCCTAAACTCCAATA630.13621916149538368No Hit
GCCACAACTGGGATTAGATACCCCTACTAGAGGAGCCTGTTCTATAAGCA610.13189474367013343No Hit
GCCACAACTGGGATTAGATACCCCGGTACCTGTCGATGAGTCTTCGGTTC610.13189474367013343No Hit
GCCACAACTGGGATTAGATACCCCCCTAACACTAGAGGAGCCTGTTCTAT580.125408116932258No Hit
GCCACAACTGGGATTAGATACCCCACGACTAGAGGAGCCTGTTCTATAAG580.125408116932258No Hit
GCCACAACTGGGATTAGATACCCCACTATGCATAACTGTAAACACAGATA570.12324590801963285No Hit
GCCACAACTGGGATTAGATACCCCAAGGAGATCGAGGGCCTAGAGGAGCC520.11243486345650718No Hit
GCCACAACTGGGATTAGATACCCCACGAGCCTAGAGGAGCCTGTTCTATA510.11027265454388202No Hit
GCCACAACTGGGATTAGATACCCCACAAGACCTAGAGGAGCCTGTTCTAT490.10594823671863177No Hit
GCCACAACTGGGATTAGATACCCCTAGTTGACTAGAGGAGCCTGTTCTAT480.10378602780600661No Hit
GCCACAACTGGGATTGATACCCCACTATGCTTAGCCCTAAACCTCAACAG470.10162381889338148No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGATT108.438797E-4295.09
CTGGGTT108.438797E-4295.08
CACTGGG400.0295.05
CTGGATT108.438797E-4295.08
AACTAGG108.438797E-4295.06
TGGATTA108.438797E-4295.09
TGGGTTA108.438797E-4295.09
ACTGGGT108.438797E-4295.07
GCCACAA45850.0294.67831
CAACTGG45400.0294.67515
AACTGGG45300.0294.67446
CTGGGAT45600.0292.08888
ACTGGGA45600.0292.08887
TGGGATT45700.0291.772439
CCACAAC46850.0287.443972
CACAACT47250.0284.386263
ACAACTG47550.0281.97164
ACACTGG500.0236.04
GCCACAC550.0214.545471
CTAGGAT150.0028408892196.666668
Produced by FastQC (version 0.11.5)